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Abstract

In this study, we tested the potential of Fourier-transform infrared absorption spectroscopy to screen, on the one hand, Saccharomyces cerevisiae and non-S. cerevisiae strains and, on the other hand, to discriminate between S. cerevisiae and Saccharomyces bayanus strains. Principal components analysis (PCA), used to compare 20 S. cerevisiae and 21 non-Saccharomyces strains, showed only 2 misclassifications. The PCA model was then used to classify spectra from 14 Samos strains. All 14 Samos strains clustered together with the S. cerevisiae group. This result was confirmed by a routinely used electrophoretic pattern obtained by pulsed-field gel electrophoresis. The method was then tested to compare S. cerevisiae and S. bayanus strains. Our results indicate that identification at the strain level is possible. This first result shows that yeast classification and S. bayanus identification can be feasible in a single measurement.

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PubMed ID:: 20921989

DOI:: 10.1139/w10-062

ScienceOpen disciplines: Chemistry

Keywords: Candida,classification,DNA, Fungal,analysis,isolation & purification,Food Industry,Genome, Fungal,Mycological Typing Techniques,Principal Component Analysis,Saccharomyces,genetics,ultrastructure,Saccharomyces cerevisiae,Sequence Analysis, DNA,Sequence Homology, Nucleic Acid,Species Specificity,Spectroscopy, Fourier Transform Infrared,Wine,microbiology

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ScienceOpen disciplines: Chemistry

Keywords: Candida, classification, DNA, Fungal, analysis, isolation & purification, Food Industry, Genome, Fungal, Mycological Typing Techniques, Principal Component Analysis, Saccharomyces, genetics, ultrastructure, Saccharomyces cerevisiae, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Species Specificity, Spectroscopy, Fourier Transform Infrared, Wine, microbiology

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FTIR spectroscopic discrimination of Saccharomyces cerevisiae and Saccharomyces bayanus strains.

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