Envelope Exchange for the Generation of Live-Attenuated Arenavirus Vaccines

Abstract

Arenaviruses such as Lassa fever virus cause significant mortality in endemic areas and represent potential bioterrorist weapons. The occurrence of arenaviral hemorrhagic fevers is largely confined to Third World countries with a limited medical infrastructure, and therefore live-attenuated vaccines have long been sought as a method of choice for prevention. Yet their rational design and engineering have been thwarted by technical limitations. In addition, viral genes had not been identified that are needed to cause disease but can be deleted or substituted to generate live-attenuated vaccine strains. Lymphocytic choriomeningitis virus, the prototype arenavirus, induces cell-mediated immunity against Lassa fever virus, but its safety for humans is unclear and untested. Using this virus model, we have developed the necessary methodology to efficiently modify arenavirus genomes and have exploited these techniques to identify an arenaviral Achilles' heel suitable for targeting in vaccine design. Reverse genetic exchange of the viral glycoprotein for foreign glycoproteins created attenuated vaccine strains that remained viable although unable to cause disease in infected mice. This phenotype remained stable even after extensive propagation in immunodeficient hosts. Nevertheless, the engineered viruses induced T cell–mediated immunity protecting against overwhelming systemic infection and severe liver disease upon wild-type virus challenge. Protection was established within 3 to 7 d after immunization and lasted for approximately 300 d. The identification of an arenaviral Achilles' heel demonstrates that the reverse genetic engineering of live-attenuated arenavirus vaccines is feasible. Moreover, our findings offer lymphocytic choriomeningitis virus or other arenaviruses expressing foreign glycoproteins as promising live-attenuated arenavirus vaccine candidates.

Synopsis

Arenaviruses such as Lassa fever virus (LFV) account for substantial mortality in endemic Third World countries and represent potential bioterrorist weapons. Live-attenuated vaccine strains would likely represent an optimal strategy for prevention, but their rational design and engineering have been thwarted by technical limitations. As an additional difficulty, arenaviruses have only four genes, all of which are needed for the infectious cycle. Unlike for other virus families, attempts at deleting a “virulence gene” would therefore interfere with the virus' viability and thereby with its immunogenicity. Using the prototype arenavirus as a model, the authors have developed the necessary tools to investigate an alternative strategy for tailoring of live-attenuated strains: Recombinants were engineered to express a foreign envelope gene instead of the natural one. Thereby, the virus was crippled but still viable. When testing such “envelope-exchange” viruses in a mouse model, they failed to cause disease. Nevertheless, they elicited rapid and long-lived immunity against overwhelming infection and lethal disease upon wild-type virus challenge. This delineates a novel general strategy for the reverse genetic engineering of live-attenuated arenavirus vaccines to be used in endemic areas or in case of a bioterrorist attack.