Carboxymethylcellulose (CMC) formed nanogels with branched poly(ethyleneimine) (bPEI) for inhibition of cytotoxicity in human MSCs as a gene delivery vehicles
There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
Specific vehicles are necessary for safe and efficient gene transfection into cells.
Nano-type hydrogels (nanogel) comprising carboxymethylcellulose (CMC) complexed with
branched type cationic poly(ethleneimine) (bPEI) were used as gene delivery vehicles.
When complexes of CMC and bPEI were used in vitro, CMC showed nano-gel type properties,
as shown by the results of a viscosity test, and bPEI showed low cytotoxicity comparing
to bPEI alone. Together, these properties are shown to maintain high gene transfection
efficiency. In viability experiments using three types of adult stem cells, cell viability
varied depending on the branch form of PEI and whether or not it is in a complex with
CMC. The gene delivery efficacy showed that the CMC nanogel complexed with bPEI (CMC-bPEI)
showed more uptaking and gene transfection ability in hMSCs comparing to bPEI alone.
In osteogenesis, the CMC-bPEI complexed with OSX pDNA showed more easy internalization
than bPEI alone complexed with OSX pDNA in hMSCs. Specific genes and proteins related
in osteogenic differentiation were expressed in hMSCs when the CMC-bPEI complexed
with OSX pDNA was used.