Human and Canine Echinococcosis Infection in Informal, Unlicensed Abattoirs in Lima, Peru

Abstract

Echinococcus granulosus infections are a major public health problem in livestock-raising regions around the world. The life cycle of this tapeworm is sustained between dogs (definitive host, canine echinococcosis), and herbivores (intermediary host, cystic hydatid disease). Humans may also develop cystic hydatid disease. Echinococcosis is endemic in rural areas of Peru; nevertheless, its presence or the extension of the problem in urban areas is basically unknown. Migration into Lima, an 8-million habitant's metropolis, creates peripheral areas where animals brought from endemic areas are slaughtered without veterinary supervision. We identified eight informal, unlicensed abattoirs in a peripheral district of Lima and performed a cross-sectional study in to assess the prevalence of canine echinococcosis, evaluated by coproELISA followed by PCR evaluation and arecoline purge. Eight of 22 dogs (36%) were positive to coproELISA, and four (18%) were confirmed to be infected with E. granulosus tapeworms either by PCR or direct observation (purge). Later evaluation of the human population living in these abattoirs using abdominal ultrasound, chest X-rays and serology, found 3 out of 32 (9.3%) subjects with echinococcal cysts in the liver (two viable, one calcified), one of whom had also lung involvement and a strongly positive antibody response. Autochthonous transmission of E. granulosus is present in Lima. Informal, unlicensed abattoirs may be sources of infection to neighbouring people in this urban environment.

Author Summary

Echinococcus granulosus infections are a major public health problem in livestock-raising regions around the world. This parasite is transmitted by dogs, and humans could be accidentally infected, developing cystic lesions in internal organs after several years of infection. The risk of infection has been widely described in Peruvian rural areas; nevertheless the extension of the problem in urban areas is basically unknown. Migration into Lima, an 8-million habitant's metropolis, creates peripheral areas where animals brought from endemic areas are slaughtered without veterinary supervision. In our study, we assess the number of infected dogs, which were living in eight informal, unlicensed abattoirs in a peripheral district of Lima, by evaluation of dog faeces using different techniques. We identified that 4 of 22 dogs were infected with E. granulosus worm. Later evaluation of the human population living in these abattoirs using abdominal ultrasound, chest X-rays and serology, found 3 of 32 subjects had echinococcal cysts in the liver, one of whom had also a cyst in lung and a positive serological test. This work demonstrates that autochthonous transmission of E. granulosus is present in Lima and that informal, unlicensed abattoirs may be sources of infection to neighbouring people in this urban environment.