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      Immunohistological demonstration of feline infectious peritonitis virus antigen in paraffin-embedded tissues using feline ascites or murine monoclonal antibodies

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          Abstract

          Feline infectious peritonitis (FIP) virus antigen was demonstrated after methanol, ethanol or formalin fixation in paraffin-embedded tissues by means of monoclonal and polyclonal antibodies. The monoclonal antibody was induced by immunization with transmissible gastroenteritis virus. Polyclonal antibodies were obtained by purification on protein A-Sepharose of ascites fluid from a cat with FIP. Almost all cats diagnosed as suffering from FIP by postmortem and histological examination exhibited FIP virus (FIPV) antigen in macrophages in granulomas whereas FIPV antigen was only once demonstrable in another location.

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          Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.

          P.L. Ey, S.J. Prowse, C.R. Jenkin (1978)
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            Peroxidase-labeled antibody. A new method of conjugation.

            P Nakane, A. Kawaoi (1974)
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              Feline coronavirus antibodies in cats.

              Diane D. Addie, O. Jarrett (1992)
              Article 0 comments Cited 26 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Vet Immunol Immunopathol
                Journal ID (iso-abbrev): Vet. Immunol. Immunopathol
                Title: Veterinary Immunology and Immunopathology
                Publisher: Published by Elsevier B.V.
                ISSN (Print): 0165-2427
                ISSN (Electronic): 1873-2534
                Publication date PMC-release: 8 December 1999
                Publication date (Print): November 1995
                Publication date (Electronic): 8 December 1999
                Volume: 49
                Issue: 1
                Pages: 177-182
                Affiliations
                [a ]Inst. Vet.-Path. Univ., Frankfurter Str. 96, D-35392 Giessen, Germany
                [b ]Natl. Vet. Inst., P.O. Box 8156, N-0033 Oslo, Norway
                [c ]Vet.-Med. Labor Univ., Winterthurerstr. 260, CH-8057 Zurich, Switzerland
                [d ]Inst. Vet.-Path. Univ., Margarete-Blank-Str. 4, D-04103 Leipzig, Germany
                Author notes
                [∗ ]Corresponding author.
                Article
                Publisher ID: 0165-2427(95)05459-J
                DOI: 10.1016/0165-2427(95)05459-J
                PMC ID: 7119862
                PubMed ID: 8588340
                SO-VID: 95da6352-0df8-495e-a42c-14504840e756
                Copyright © Copyright © 1995 Published by Elsevier B.V.
                License:

                Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

                History
                Date accepted : 20 March 1995
                Categories
                Subject: Article

                ScienceOpen disciplines: Veterinary medicine
                Keywords: cat disease,feline infectious peritonitis (fip),feline coronavirus,monoclonal antibodies
                Data availability:
                ScienceOpen disciplines: Veterinary medicine
                Keywords: cat disease, feline infectious peritonitis (fip), feline coronavirus, monoclonal antibodies

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