For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
83
views
6
references
 Top references
cited by
103
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
1 collections
    0
    shares
      77
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Genetic Variation among African Swine Fever Genotype II Viruses, Eastern and Central Europe

      brief-report

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          African swine fever virus (ASFV) was first reported in eastern Europe/Eurasia in 2007. Continued spread of ASFV has placed central European countries at risk, and in 2014, ASFV was detected in Lithuania and Poland. Sequencing showed the isolates are identical to a 2013 ASFV from Belarus but differ from ASFV isolated in Georgia in 2007.

          Related collections

          Most cited references6

          • Record: found
          • Abstract: found
          • Article: not found

          Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes.

          Edward Okoth, Jackline Kasiti, Evans A Taracha … (2009)
          Complete sequencing of p54-gene from 67 European, American, and West and East African Swine Fever virus (ASFV) isolates revealed that West African and European ASFV isolates classified within the predominant Genotype I according to partial sequencing of p72 were discriminated into four major sub-types on the basis of their p54 sequences. This highlighted the value of p54 gene sequencing as an additional, intermediate-resolution, molecular epidemiological tool for typing of ASFV viruses. We further evaluated p54-based genotyping, in combination with partial sequences of two other genes, for determining the genetic relationships and origin of viruses responsible for disease outbreaks in Kenya. Animals from Western and central Kenya were confirmed as being infected with ASFV using a p72 gene-based PCR assay, following outbreaks of severe hemorrhagic disease in domestic pigs in 2006 and 2007. Eleven hemadsorbing viruses were isolated in macrophage culture and genotyped using a combination of full-length p54-gene sequencing, partial p72-gene sequencing, and analysis of tetrameric amino acid repeat regions within the variable region of the B602L gene (CVR). The data revealed that these isolates were identical in their p72 and p54 sequence to viruses responsible for ASF outbreaks in Uganda in 2003. There was a minor difference in the number of tetrameric repeats within the B602L sequence of the Kenyan isolates that caused the second Kenyan outbreak in 2007. A practical implication of the genetic similarity of the Kenyan and Ugandan viral isolates is that ASF control requires a regional approach.
          Article 0 comments Cited 78 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Molecular epidemiology of African swine fever virus studied by analysis of four variable genome regions.

            J. Blanco, Matthew Dixon, G Hutchings … (2006)
            Variable regions of the African swine fever virus genome, which contain arrays of tandem repeats, were compared in the genomes of isolates obtained over a 40-year period. Comparison of the size of products generated by polymerase chain reaction (PCR) from four different genome regions, within the B602L and KP86R genes and intergenic regions J286L and BtSj, placed 43 closely related isolated from Europe, the Caribbean, West and Central Africa into 17 different virus sub-groups. Sequence analysis of the most variable fragment, within the B602L gene, from 81 different isolates distinguished 31 sub-groups of virus isolates which varied in sequence and number of a tandem repeat encoding 4 amino acids. Thus, each of these analysis methods enabled isolates, which were previously grouped together by sequencing of a more conserved genome region, to be separated into multiple sub-groups. This provided additional information about strains of viruses circulating in different countries. The methods could be used in future to study the epidemiology and evolution of virus isolates and to trace the sources of disease outbreaks.
            Article 0 comments Cited 54 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site.

              Marisa Arias, Raquel Martin-Iguacel, Elena Martin … (2011)
              The role of the ancestral sylvatic cycle of the African swine fever virus (ASFV) is not well understood in the endemic areas of eastern Africa. We therefore analysed the ASF infection status on samples collected from 51 free-ranging warthogs (Phacocherus africanus) and 1576 Ornithodorus porcinus ticks from 26 independent warthog burrows at a single ranch in Kenya. Abattoir samples from 83 domestic pigs without clinical symptoms, originating from specific locations with no recent reported ASF outbreaks were included in this study. All samples were derived from areas of central Kenya, where ASF outbreaks have been reported in the past. Infection with ASFV was confirmed in 22 % of O. porcinus pools, 3.22 % of adult warthog serum samples and 49 % of domestic pig serum samples by using p72-based PCR. All of the warthog sera were positive for anti-ASFV antibodies, investigated by using ELISA, but none of the domestic pig sera were positive. Twenty O. porcinus-, 12 domestic pig- and three warthog-derived viruses were genotyped at four polymorphic loci. The ASFV isolates from ticks and domestic pigs clustered within p72 genotype X. By contrast, ASF viruses genotyped directly from warthog sera, at same locality as the tick isolates, were within p72 genotype IX and genetically similar to viruses causing recent ASF outbreaks in Kenya and Uganda. This represents the first report of the co-existence of different ASFV genotypes in warthog burrow-associated ticks and adult wild warthogs. The data from this and earlier studies suggest transfer of viruses of at least two different p72 genotypes, from wild to domestic pigs in East Africa.
              Article 0 comments Cited 29 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Emerg Infect Dis
                Journal ID (iso-abbrev): Emerging Infect. Dis
                Journal ID (publisher-id): EID
                Title: Emerging Infectious Diseases
                Publisher: Centers for Disease Control and Prevention
                ISSN (Print): 1080-6040
                ISSN (Electronic): 1080-6059
                Publication date (Print): September 2014
                Volume: 20
                Issue: 9
                Pages: 1544-1547
                Affiliations
                [1]Centro de Investigación en Sanidad Animal (CISA-INIA), Madrid, Spain (C. Gallardo, J. Fernández-Pinero, V. Pelayo, R. Nieto, P. Fernández-Pacheco, C. Pérez, A. Soler, M. Arias);
                [2]National Institute of Veterinary Virology and Microbiology, Pokrov, Russia (I. Gazaev, D. Kolvasov);
                [3]National Veterinary Research Institute, Pulawy, Poland (I. Markowska-Daniel);
                [4]National Food and Veterinary Risk Assessment Institute, Vilnius, Lithuania (G. Pridotkas);
                [5]Belarusian State Veterinary Centre, Minsk, Belarus (S. Bokhan);
                [6]State Research Institute of Laboratory Diagnostic and Veterinary Sanitary Expertise, Kiev, Ukraine (O. Nevolko, Z. Drozhzhe)
                Author notes
                Address for correspondence: Carmina Gallardo, CISA-INIA. Ctra Algete el Casar s/n. 28130 Valdeolmos, Madrid, Spain; email: gallardo@ 123456inia.es
                Article
                Publisher ID: 14-0554
                DOI: 10.3201/eid2009.140554
                PMC ID: 4178389
                PubMed ID: 25148518
                SO-VID: 7e80a0c3-024c-4e01-95cf-840c39b30e9b
                History
                Categories
                Subject: Dispatch
                Subject: Dispatch

                ScienceOpen disciplines: Infectious disease & Microbiology
                Keywords: african swine fever,genotype ii,intergenic regions,genetic variability
                Data availability:
                ScienceOpen disciplines: Infectious disease & Microbiology
                Keywords: african swine fever, genotype ii, intergenic regions, genetic variability

                Comments

                Comment on this article

                scite_

                Similar content77

                See all similar

                Cited by100

                See all cited by

                Most referenced authors53

                See all reference authors