Transcriptomic and metabolomic data of goat ovarian and uterine tissues during sexual maturation

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Abstract

The ovaries and uterus are crucial reproductive organs in mammals, and their coordinated development ensures the normal development of sexual maturity and reproductive capacity. This study aimed to comprehensively capture the different physiological stages of the goat’s sexual maturation by selecting four specific time points. We collected samples of ovarian and uterine tissues from five female Jining Gray goats at each time point: after birth (D1), 2-month-old (M2), 4-month-old (M4), and 6-month-old (M6). By combining transcriptomic sequencing of 40 samples (including rRNA-depleted RNA-seq libraries with 3607.8 million reads and miRNA-seq libraries with 444.0 million reads) and metabolomics analysis, we investigated the transcriptomic mechanisms involved in reproductive regulation in the ovary and uterus during sexual maturation, as well as the changes in metabolites and their functional potential. Additionally, we analyzed blood hormone indices and uterine tissue sections to examine temporal changes. These datasets will provide a valuable reference for the reproductive regulation of the ovary and uterus, as well as the regulation of metabolites during sexual maturation in goats.

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Most cited references 27

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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

Piotr Chomczynski, Nicoletta Sacchi (1987)

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Purification of RNA using TRIzol (TRI reagent).

Donald Rio, Manuel Ares, Gregory Hannon … (2010)

TRIzol solubilization and extraction is a relatively recently developed general method for deproteinizing RNA. This method is particularly advantageous in situations where cells or tissues are enriched for endogenous RNases or when separation of cytoplasmic RNA from nuclear RNA is impractical. TRIzol (or TRI Reagent) is a monophasic solution of phenol and guanidinium isothiocyanate that simultaneously solubilizes biological material and denatures protein. After solubilization, the addition of chloroform causes phase separation (much like extraction with phenol:chloroform:isoamyl alcohol), where protein is extracted to the organic phase, DNA resolves at the interface, and RNA remains in the aqueous phase. Therefore, RNA, DNA, and protein can be purified from a single sample (hence, the name TRIzol). TRIzol extraction is also an effective method for isolating small RNAs, such as microRNAs, piwi-associated RNAs, or endogeneous, small interfering RNAs. However, TRIzol is expensive and RNA pellets can be difficult to resuspend. Thus, the use of TRIzol is not recommend when regular phenol extraction is practical.

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The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on.

Piotr Chomczynski, Nicoletta Sacchi (2006)

Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is separated from DNA after extraction with an acidic solution containing guanidinium thiocyanate, sodium acetate, phenol and chloroform, followed by centrifugation. Under acidic conditions, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol and can be used for several applications. The original protocol, enabling the isolation of RNA from cells and tissues in less than 4 hours, greatly advanced the analysis of gene expression in plant and animal models as well as in pathological samples, as demonstrated by the overwhelming number of citations the paper gained over 20 years.

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Author and article information

Contributors

Tianle Chao:

ORCID: http://orcid.org/0000-0001-9721-0634

chaotianle@sdau.edu.cn

Journal

Journal ID (nlm-ta): Sci Data

Journal ID (iso-abbrev): Sci Data

Title: Scientific Data

Publisher: Nature Publishing Group UK (London )

ISSN (Electronic): 2052-4463

Publication date (Electronic): 13 July 2024

Publication date PMC-release: 13 July 2024

Publication date Collection: 2024

Volume: 11

Electronic Location Identifier: 777

Affiliations

[1 ]Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, ( https://ror.org/02ke8fw32) Tai’an, Shandong China

[2 ]GRID grid.440622.6, ISNI 0000 0000 9482 4676, Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, , Shandong Agricultural University, ; Tai’an, Shandong China

Author information

Tianle Chao http://orcid.org/0000-0001-9721-0634

Article

Publisher ID: 3565

DOI: 10.1038/s41597-024-03565-w

PMC ID: 11246480

PubMed ID: 39003290

SO-VID: 76ae6eed-aad5-491e-8ce7-8d7eb4fad023

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

History

Date received : 18 September 2023

Date accepted : 24 June 2024

Funding

Funded by: The Project of Improved Agricultural Varieties in Shandong Province (2021LZGC010) The Youth Project of Shandong Provincial Natural Science Foundation (ZR2023QC044) The Shandong Provincial Sheep and Goat Industry Technology System (SDAIT-10-01) The Funds of The Shandong “Double Tops” Program (SYL2017YSTD12) The Project of Improved Agricultural Varieties in Shandong Province (2019LZGC012)

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Keywords: transcriptomics,metabolomics

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Keywords: transcriptomics, metabolomics

Transcriptomic and metabolomic data of goat ovarian and uterine tissues during sexual maturation

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Most cited references 27

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

Purification of RNA using TRIzol (TRI reagent).

The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on.

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