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      Diagnostic utility of BINAX NOW RSV – an evaluation of the diagnostic performance of BINAX NOW RSV in comparison with cell culture and direct immunofluorescence

      research-article
      Author(s): 1 ,
      Publication date (Electronic):
      Journal: Annals of Clinical Microbiology and Antimicrobials
      Publisher: BioMed Central

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          Abstract

          Background

          The regular increase in the incidence of respiratory illness caused by respiratory syncytial virus (RSV) during winter months in the United Kingdom, and other countries with temperate climate is usually accompanied by increased bed pressures especially in paediatric units in these countries. As a result, there is usually an increase in the demand for infection control services during these months. This makes obvious the need for making a rapid diagnosis of the infection during these months. BINAX NOW RSV (Maine, USA), a rapid membrane based immunochromatographic assay was designed to achieve this objective.

          Methods

          This study evaluated the diagnostic performance of BINAX NOW RSV in comparison with the methods routinely used in our laboratory namely direct immunofluorescence (DIF) and cell culture.

          Results and conclusion

          Results indicate that Binax Now RSV could be relied on to make infection control decisions in paediatric units during periods of peak RSV activity.

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          Most cited references7

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          Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ assays for detection of respiratory syncytial virus.

          Peter J S Smith, B. von Haller, P Nassos (2004)
          The Binax NOW assay (Binax, Inc., Portland, Maine) and the BD Directigen EZ assay (Becton Dickinson and Company, Sparks, Md.), two new rapid immunoassays for detection of respiratory syncytial virus (RSV), as well as the BD Directigen RSV assay (DRSV) (Becton Dickinson and Company) and direct immunofluorescence staining (DFA) were compared with culture for detection of RSV in fresh specimens from both children and adults during the 2002-2003 respiratory virus season. The majority (95%) of specimens were nasal or nasopharyngeal washes or aspirates. A total of 47 (26%) were culture positive for RSV. The overall sensitivities of DFA (n = 149), NOW (n = 118), EZ (n = 88), and DRSV (n = 180) compared with culture (n = 180) were 93, 89, 59, and 77%, respectively. The specificities of DFA, NOW, EZ, and DRSV were 97, 100, 98, and 96%, respectively. However, when results were separated into those from children and those from adults, DFA was the only rapid test adequate for detection of RSV (sensitivity of 100% compared to 0, 0, and 25% for NOW, EZ, and DRSV, respectively) in adults. For children the sensitivities of DFA, NOW, EZ, and DRSV were 93, 94, 72, and 81%. The NOW assay was the most sensitive and specific and the easiest to perform of the kit tests for detecting RSV in children. None of these three rapid kit tests was sensitive for detecting RSV in specimens from adults. DFA remains the rapid method of choice for detecting RSV in the adult population.
          Article 0 comments Cited 14 times – based on 0 reviews     Review now
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            Laboratory diagnosis of respiratory virus infections in 24 hours by utilizing shell vial cultures.

            J Preuss, H. Engler (1997)
            Immunofluorescence staining of centrifugation-enhanced shell vial (SV) cultures for respiratory viruses (RV) after 24 h of incubation, rather than the more commonly prescribed times of 48 h and 5 days, allowed for the detection of 77% of the RV-positive specimens that would ordinarily not have been detected as positive until 48 h. Staining SVs at 24 h also permitted earlier detection of viruses that were missed by rapid antigen detection methods.
            Article 0 comments Cited 7 times – based on 0 reviews     Review now
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              Respiratory Syncytial Virus

              Timothy R Peters, James Crowe (2008)
              Article 0 comments Cited 2 times – based on 0 reviews
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                Author and article information

                Journal
                Journal ID (nlm-ta): Ann Clin Microbiol Antimicrob
                Title: Annals of Clinical Microbiology and Antimicrobials
                Publisher: BioMed Central (London )
                ISSN (Electronic): 1476-0711
                Publication date Collection: 2006
                Publication date (Electronic): 6 June 2006
                Volume: 5
                Page: 13
                Affiliations
                [1 ]Health Protection Agency, Birmingham Heartlands Hospital, Bordesley Green East, Birmingham, B9 5SS, UK
                Article
                Publisher ID: 1476-0711-5-13
                DOI: 10.1186/1476-0711-5-13
                PMC ID: 1513596
                PubMed ID: 16756663
                SO-VID: 6dbf5d5a-25c5-4872-9518-39128f496b8c
                Copyright © Copyright © 2006 Jonathan; licensee BioMed Central Ltd.
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 22 March 2006
                Date accepted : 6 June 2006
                Categories
                Subject: Research

                ScienceOpen disciplines: Infectious disease & Microbiology
                Data availability:
                ScienceOpen disciplines: Infectious disease & Microbiology

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