9
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Hypercoagulation detected by routine and global laboratory hemostasis assays in patients with infective endocarditis

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background

          Coagulation system is heavily involved into the process of infective endocarditis (IE) vegetation formation and can facilitate further embolization. In this study we aimed to assess the coagulation and platelet state in IE implementing a wide range of standard and global laboratory assays. We also aim to determine whether prothrombotic genetic polymorphisms play any role in embolization and mortality in IE patients.

          Methods

          37 patients with IE were enrolled into the study. Coagulation was assessed using standard coagulation assays (activated partial thromboplastin time (APTT), prothrombin, fibrinogen, D-dimer concentrations) and integral assays (thromboelastography (TEG) and thrombodynamics (TD)). Platelet functional activity was estimated by flow cytometry. Single nuclear polymorphisms of coagulation system genes were studied.

          Results

          Fibrinogen concentration and fibrinogen-dependent parameters of TEG and TD were increased in patients indicating systemic inflammation. In majority of patients clot growth rate in thrombodynamics was significantly shifted towards hypercoagulation in consistency with D-dimers elevation. However, in some patients prothrombin, thromboelastography and thrombodynamics were shifted towards hypocoagulation. Resting platelets were characterized by glycoprotein IIb-IIIa activation and degranulation. In patients with fatal IE, we observed a significant decrease in fibrinogen and thrombodynamics. In patients with embolism, we observed a significant decrease in the TEG R parameter. No association of embolism or mortality with genetic polymorphisms was found in our cohort.

          Conclusions

          Our findings suggest that coagulation in patients with infective endocarditis is characterized by general hypercoagulability and platelet pre-activation. Some patients, however, have hypocoagulant coagulation profile, which presumably can indicate progressing of hypercoagulation into consumption coagulopathy.

          Related collections

          Most cited references42

          • Record: found
          • Abstract: found
          • Article: not found

          A new method of classifying prognostic comorbidity in longitudinal studies: Development and validation

          The objective of this study was to develop a prospectively applicable method for classifying comorbid conditions which might alter the risk of mortality for use in longitudinal studies. A weighted index that takes into account the number and the seriousness of comorbid disease was developed in a cohort of 559 medical patients. The 1-yr mortality rates for the different scores were: "0", 12% (181); "1-2", 26% (225); "3-4", 52% (71); and "greater than or equal to 5", 85% (82). The index was tested for its ability to predict risk of death from comorbid disease in the second cohort of 685 patients during a 10-yr follow-up. The percent of patients who died of comorbid disease for the different scores were: "0", 8% (588); "1", 25% (54); "2", 48% (25); "greater than or equal to 3", 59% (18). With each increased level of the comorbidity index, there were stepwise increases in the cumulative mortality attributable to comorbid disease (log rank chi 2 = 165; p less than 0.0001). In this longer follow-up, age was also a predictor of mortality (p less than 0.001). The new index performed similarly to a previous system devised by Kaplan and Feinstein. The method of classifying comorbidity provides a simple, readily applicable and valid method of estimating risk of death from comorbid disease for use in longitudinal studies. Further work in larger populations is still required to refine the approach because the number of patients with any given condition in this study was relatively small.
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            2015 ESC Guidelines for the management of infective endocarditis: The Task Force for the Management of Infective Endocarditis of the European Society of Cardiology (ESC). Endorsed by: European Association for Cardio-Thoracic Surgery (EACTS), the European Association of Nuclear Medicine (EANM).

              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Infective Endocarditis in Adults: Diagnosis, Antimicrobial Therapy, and Management of Complications: A Scientific Statement for Healthcare Professionals From the American Heart Association.

              Infective endocarditis is a potentially lethal disease that has undergone major changes in both host and pathogen. The epidemiology of infective endocarditis has become more complex with today's myriad healthcare-associated factors that predispose to infection. Moreover, changes in pathogen prevalence, in particular a more common staphylococcal origin, have affected outcomes, which have not improved despite medical and surgical advances.
                Bookmark

                Author and article information

                Contributors
                Role: Data curationRole: Formal analysisRole: Funding acquisitionRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: InvestigationRole: ResourcesRole: Writing – original draft
                Role: ConceptualizationRole: InvestigationRole: Project administrationRole: ResourcesRole: Writing – original draft
                Role: InvestigationRole: Resources
                Role: Investigation
                Role: Investigation
                Role: ResourcesRole: Supervision
                Role: Data curationRole: Investigation
                Role: MethodologyRole: Writing – review & editing
                Role: ConceptualizationRole: Writing – review & editing
                Role: SupervisionRole: Writing – review & editing
                Role: ConceptualizationRole: Project administrationRole: SupervisionRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS One
                plos
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                15 December 2021
                2021
                : 16
                : 12
                : e0261429
                Affiliations
                [1 ] Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russian Federation
                [2 ] Center for Theoretical Problems of Physicochemical Pharmacology, Moscow, Russian Federation
                [3 ] City Clinical Hospital named after V.V. Vinogradov, Moscow, Russia Federation
                [4 ] Peoples’ Friendship University of Russia (RUDN), Moscow, Russia Federation
                [5 ] Lomonosov Moscow State University, Moscow, Russian Federation
                [6 ] Moscow Institute of Physics and Technology, Dolgoprudny, Russian Federation
                Universite de Liege (B34), BELGIUM
                Author notes

                Competing Interests: We disclose a conflict of interest for Prof. Fazoil Ataullakhanov, who is a co-founder of HemaCore LLC, which holds several patents and patent applications that are related to the diagnostic use of spatial clot growth and which has developed the assay under the trade name of Thrombodynamics ® (Ataullakhanov FI, international patent applications PCT/CH2007/000543 filing date 02.11.2007 and РСТ/RU2012/000570 filing date 16.07.2012). This does not alter our adherence to PLOS ONE policies on sharing data and materials.

                Author information
                https://orcid.org/0000-0003-0167-6726
                https://orcid.org/0000-0003-1541-9480
                https://orcid.org/0000-0001-6905-2878
                https://orcid.org/0000-0002-5873-1768
                Article
                PONE-D-21-18318
                10.1371/journal.pone.0261429
                8673624
                34910783
                5e0209bd-e381-4d23-8ea9-4bb8ae142237
                © 2021 Koltsova et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 3 June 2021
                : 1 December 2021
                Page count
                Figures: 5, Tables: 2, Pages: 19
                Funding
                Funded by: funder-id http://dx.doi.org/10.13039/501100006769, Russian Science Foundation;
                Award ID: 20-75-00057
                Award Recipient :
                The study was supported by a grant from the Russian Science Foundation https://www.rscf.ru/ (project No. 20-75-00057) to EMK. The funder did not play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
                Categories
                Research Article
                Biology and Life Sciences
                Anatomy
                Body Fluids
                Blood
                Platelets
                Medicine and Health Sciences
                Anatomy
                Body Fluids
                Blood
                Platelets
                Biology and Life Sciences
                Physiology
                Body Fluids
                Blood
                Platelets
                Biology and Life Sciences
                Cell Biology
                Cellular Types
                Animal Cells
                Blood Cells
                Platelets
                Medicine and Health Sciences
                Hematology
                Blood Coagulation
                Platelet Activation
                Medicine and Health Sciences
                Pharmaceutics
                Drug Therapy
                Cardiovascular Therapy
                Anticoagulant Therapy
                Medicine and Health Sciences
                Cardiology
                Endocarditis
                Medicine and Health Sciences
                Hematology
                Hemostasis
                Medicine and Health Sciences
                Pharmaceutics
                Drug Therapy
                Antiplatelet Therapy
                Biology and Life Sciences
                Biochemistry
                Glycobiology
                Glycoproteins
                Fibrinogen
                Biology and Life Sciences
                Biochemistry
                Enzymology
                Enzymes
                Proteases
                Serine Proteases
                Biology and Life Sciences
                Biochemistry
                Proteins
                Enzymes
                Proteases
                Serine Proteases
                Custom metadata
                All files are available from the Harvard dataverse ( https://doi.org/10.7910/DVN/8U4AV5).

                Uncategorized
                Uncategorized

                Comments

                Comment on this article