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ADCC Develops Over Time during Persistent Infection with Live-Attenuated SIV and Is Associated with Complete Protection against SIV mac251 Challenge
research-article
Author(s):
Michael D. Alpert
1 ,
Jackson D. Harvey
1 ,
W. Anderson Lauer
1 ,
R. Keith Reeves
2 ,
Michael Piatak Jr.
3 ,
Angela Carville
4 ,
Keith G. Mansfield
4 ,
Jeffrey D. Lifson
3 ,
Wenjun Li
5 ,
Ronald C. Desrosiers
1 ,
R. Paul Johnson
2
,
6 ,
David T. Evans
1
,
*
Publication date (Electronic):
23 August 2012
Journal:
PLoS Pathogens
Publisher:
Public Library of Science
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Abstract
Live-attenuated strains of simian immunodeficiency virus (SIV) routinely confer apparent sterilizing immunity against pathogenic SIV challenge in rhesus macaques. Understanding the mechanisms of protection by live-attenuated SIV may provide important insights into the immune responses needed for protection against HIV-1. Here we investigated the development of antibodies that are functional against neutralization-resistant SIV challenge strains, and tested the hypothesis that these antibodies are associated with protection. In the absence of detectable neutralizing antibodies, Env-specific antibody-dependent cell-mediated cytotoxicity (ADCC) emerged by three weeks after inoculation with SIVΔ nef, increased progressively over time, and was proportional to SIVΔ nef replication. Persistent infection with SIVΔ nef elicited significantly higher ADCC titers than immunization with a non-persistent SIV strain that is limited to a single cycle of infection. ADCC titers were higher against viruses matched to the vaccine strain in Env, but were measurable against viruses expressing heterologous Env proteins. In two separate experiments, which took advantage of either the strain-specificity or the time-dependent maturation of immunity to overcome complete protection against SIV mac251 challenge, measures of ADCC activity were higher among the SIVΔ nef-inoculated macaques that remained uninfected than among those that became infected. These observations show that features of the antibody response elicited by SIVΔ nef are consistent with hallmarks of protection by live-attenuated SIV, and reveal an association between Env-specific antibodies that direct ADCC and apparent sterilizing protection by SIVΔ nef.
Author Summary
Live-attenuated vaccines can prevent simian immunodeficiency virus (SIV) infection upon experimental challenge of rhesus macaques. Although safety considerations preclude vaccinating humans with live-attenuated HIV-1, it may be possible to replicate the types of immunity induced by live-attenuated SIV through an alternative approach. Thus, identifying the immune responses underlying protection by live-attenuated SIV and understanding their induction would provide guidance for HIV-1 vaccine design. An important role for the maturation of virus-specific antibody responses could explain the time-dependent development of protection by live-attenuated SIV. However, antibodies that block the entry of the challenge virus into cells are usually undetectable. Antibodies can also direct the killing of virus-infected cells by antibody-dependent cell-mediated cytotoxicity (ADCC). Here we show that live-attenuated SIV induces progressive increases in ADCC over time, and that the development of these antibodies is dependent upon the persistent replication of the vaccine strain. In two different experiments, the animals immunized with live-attenuated SIV that remained uninfected after pathogenic SIV challenge had higher measures of ADCC than those that became infected. Our results suggest that antibodies contribute to protection by live-attenuated SIV, and that persistent stimulation of antibody responses may be essential for HIV-1 vaccines to induce high ADCC activity.
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Most cited references54
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Ren-Feng Guo, Peter Ward (2005)
The complement system not only represents an effective innate immune mechanism of host defense to eradicate microbial pathogens, but it is also widely involved in many forms of acute and chronic inflammatory diseases including sepsis, acute lung injury, ischemia-reperfusion injury, and asthma, to give just a few examples. The complement-activated product, C5a, displays powerful biological activities that lead to inflammatory sequelae. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accumulating data suggest that C5a provides a vital bridge between innate and adaptive immune functions, extending the roles of C5a in inflammation. Herein, we review human and animal data describing the cellular and molecular mechanisms of C5a in the development of inflammatory disorders, sepsis, acute lung injury, ischemia-reperfusion injury, and asthma.
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Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding.
U. O'Doherty, W J Swiggard, M Malim (2000)
The study of early events in the human immunodeficiency virus type 1 (HIV-1) life cycle can be limited by the relatively low numbers of cells that can be infected synchronously in vitro. Although the efficiency of HIV-1 infection can be substantially improved by centrifugal inoculation (spinoculation or shell vial methods), the underlying mechanism of enhancement has not been defined. To understand spinoculation in greater detail, we have used real-time PCR to quantitate viral particles in suspension, virions that associate with cells, and the ability of those virions to give rise to reverse transcripts. We report that centrifugation of HIV-1(IIIB) virions at 1,200 x g for 2 h at 25 degrees C increases the number of particles that bind to CEM-SS T-cell targets by approximately 40-fold relative to inoculation by simple virus-cell mixing. Following subsequent incubation at 37 degrees C for 5 h to allow membrane fusion and uncoating to occur, the number of reverse transcripts per target cell was similarly enhanced. Indeed, by culturing spinoculated samples for 24 h, approximately 100% of the target cells were reproducibly shown to be productively infected, as judged by the expression of p24(gag). Because the modest g forces employed in this procedure were found to be capable of sedimenting viral particles and because CD4-specific antibodies were effective at blocking virus binding, we propose that spinoculation works by depositing virions on the surfaces of target cells and that diffusion is the major rate-limiting step for viral adsorption under routine in vitro pulsing conditions. Thus, techniques that accelerate the binding of viruses to target cells not only promise to facilitate the experimental investigation of postentry steps of HIV-1 infection but should also help to enhance the efficacy of virus-based genetic therapies.
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Broadly Neutralizing Human Anti-HIV Antibody 2G12 Is Effective in Protection against Mucosal SHIV Challenge Even at Low Serum Neutralizing Titers
Ann Hessell, Eva Rakasz, Pascal Poignard … (2009)
Introduction There is widespread acceptance that eliciting neutralizing antibodies is likely to be an important goal of an effective HIV vaccine [1],[2],[3]. A good correlation is generally reported between the ability of an antibody to neutralize in vitro and to protect in vivo against HIV in animal models [4],[5],[6],[7],[8],[9]. The most quantitative studies have titrated the ability of specific antibodies to protect and found that sterilizing immunity is achieved when the serum concentration of antibody in the challenged animals is many multiples of the in vitro neutralization titer [4],[8],[10]. For instance, Nishimura, et al. reported that 99% of macaques were protected against intravenous challenge with an R5 SHIVDH12 by a specific polyclonal antibody at a 100% neutralization titer of 1∶38 [10]. In another example, we have reported sterilizing immunity against R5 SHIVSF162P4 vaginal challenge in 4/4 macaques with a dose of the broadly neutralizing human antibody b12 yielding a serum neutralizing titer of about 1∶400 at challenge [8]. The titer corresponded to 90% neutralization in a PBMC assay. Nishimura et al [10] estimated that this titer corresponded to 1∶32.5 or greater in their assay system providing good correspondence between the two studies. At an antibody dose giving a serum neutralizing titer of about 1∶80 in the Parren, et al. study, 2/4 macaques showed sterilizing immunity and the other 2 were infected with a delayed and lower primary viremia as compared to controls. At an antibody dose giving a serum neutralizing titer of about 1∶16, no animal was protected but there was a slight delay and some lowering in the magnitude of primary viremia. Most other studies have not titrated the ability of antibodies to protect but high serum concentrations of antibody relative to neutralizing titer were generally used and shown to provide protection against virus challenge [4],[5],[6],[9],[11]. The one notable exception is provided by studies of Mascola and colleagues [7] on protection by the broadly neutralizing human MAb 2G12. In particular 2/4 macaques showed sterilizing immunity when challenged by an ×4 SHIV (SHIV89.6P) when the serum neutralizing titer, as measured at 90% neutralization in a PBMC assay, was less than 9. In fact, the mean concentration of 2G12 in the sera of the animals at challenge was calculated to provide 90% neutralization only with neat serum (i.e. 1∶1 neutralizing titer). The actual concentration of 2G12 in the protected animals at the time of challenge was relatively high, about 200 µg/ml following an i.v. administration of 15 mg/kg antibody, but 2G12 is relatively poor at neutralization of SHIV89.6P (IC90∼200 µg/ml) hence the low neutralizing titer. The authors also carried out protection experiments with mixtures of antibodies, including 2G12. These experiments when taken together again suggested that 2G12 may provide protection that is unusually effective relative to its neutralizing titer. Monoclonal human IgG1 2G12 is a very interesting and unique antibody. It is broadly neutralizing, particularly against clade B HIV-1 isolates [12],[13],[14]. It has a domain-exchanged structure that leads to closely proximal antibody combining sites that are well suited to the recognition of a cluster of oligomannose residues on the glycan shield of HIV [12],[15],[16],[17],[18]. 2G12 belongs to a small set of human MAbs that are described as broadly neutralizing and that recognize distinct epitopes on the HIV envelope spike. The MAb b12 recognizes an epitope overlapping the CD4 binding site “on the side” of the spike and the MAbs 2F5, 4E10 and Z13e1 recognize gp41 very close to the viral membrane, whilst 2G12 recognizes an epitope which is more on the “top” of the spike [19],[20],[21]. Given the suggestion that 2G12 may have unusual prophylactic activities and given the potential importance of this for HIV vaccine design, we decided to carry out a macaque protection study using a virus different from that of Mascola and colleagues and to pursue potential properties of 2G12 that might correlate with protection. Ideally, we would have had available a SHIV that was relatively neutralization sensitive to 2G12 to permit study of a maximum dynamic range of 2G12 concentrations with neutralizing activity. However, currently available SHIVs are relatively resistant to 2G12 and the R5 virus SHIVSF162P3 was chosen as the most sensitive to 2G12 neutralization. An R5 virus was thought to be more appropriate for modeling human infection than an ×4 virus. The challenge virus was used intravaginally following pre-administration of 2G12 intravenously. The results indicate that 2G12 can provide protection against an R5 virus challenge at a surprisingly low neutralization titer. Unusually efficient transport to the vaginal mucosal surface does not appear to explain the activity of 2G12. The results support targeting the glycan shield through vaccine design. Results The ability of 2G12 to neutralize a panel of SHIVs in PBMC and pseudovirus assays was first assessed. A comparison with b12 was included in the study. As shown in Table 1, 2G12 was not particularly effective against any of the SHIVs tested. The activity of 2G12 against the R5 SHIVSF162P3 was comparable to that against the ×4 SHIV89.6P used in previous studies described above and was chosen for macaque studies. 10.1371/journal.ppat.1000433.t001 Table 1 Comparison of SHIV neutralization by b12 and 2G12 in rhesus PBMC-based and pseudovirus luciferase reporter gene assays. Virus MAb Rhesus PBMC-based Neutralization Assay Pseudovirus-based Neutralization Assay (µg/ml) IC50 IC90 IC50 IC90 SHIV 89.6P 2G12 20 >900 2.6 >50 b12 300 >900 11.5 >50 SHIV SF162P3 2G12 20 900 7.6 >50 b12 2 8 0.29 2 SHIV BaL 2G12 >300 >300 1 >50 b12 <1.2 <1.2 0.02 0.09 The selection of SHIVSF162P3 for the protection study was based on the results of 2G12 neutralization of rhesus PBMCs and pseudovirus assays against the panel shown. Figure 1 depicts the outcome of the protection study that was carried out with five 2G12-treated animals, two antibody isotype (anti-Dengue NS1 IgG1, DEN3) treated control animals and two antibody-untreated control animals. The Indian rhesus macaques were first treated with Depo-Provera to thin the vaginal epithelium and to synchronize menstrual cycles [8],[22]. One day before vaginal challenge with 500 TCID50 (50% tissue culture infectious doses) of SHIVSF162P3, each animal was given an intravenous dose of 40 mg/kg of either 2G12 or the isotype control antibody. Prior to the protection experiment, two additional control animals were challenged with 500 TCID50 SHIVSF162P3 without administration of antibody to verify the infectivity of the viral stock. Blood was drawn from the animals at regular intervals following challenge to monitor viral infection, serum levels of passively administered antibody and serum neutralizing activity. The 4 control animals became infected with peak viremias of approximately 107 virus copies per ml between days 14 and 21 as is generally noted in this system [9]. Two of the five 2G12-treated animals also became infected. One was infected with viral kinetics closely similar to that of the control animals. The second had a notably delayed and lower peak viremia at day 35. Three of the five 2G12-treated animals were protected and showed no detectable viremia at day 55. In order to determine whether breakthrough infection may be associated with selection of antibody escape mutants, we attempted to sequence the env gene from plasma virus of the unprotected animals. Env from animal 95113 could not be amplified but interestingly sequence analysis of animal 90154 env revealed a T388A mutation disrupting the position 386 N-glycosylation and consistent with 2G12 escape. The characterization of a 2G12 escape mutant in one of the unprotected animals raises the possibility that such variants already exist in the inoculum. Alternatively, in the presence of Ab, in particular at the suboptimal concentration achieved in the study, a certain level of viral replication may take place following challenge, allowing in some cases for the generation and selection of an escape mutant. Such scenario would suggest that the mechanism of antibody protection is not only to prevent cell infection but also to abort an already ongoing infection, presumably though effector functions as discussed below. These two possible scenarios for escape are currently under investigation. 10.1371/journal.ppat.1000433.g001 Figure 1 Plasma viral loads following SHIVSF162P3 vaginal challenge of 2G12-treated and control macaques. A total of nine female Indian rhesus macaques were divided into treatment groups of five animals for i.v. administration of 2G12, two animals to receive the isotype control (Dengue anti-NS1, DEN3), and two additional controls were challenged prior to the beginning of the protection study to confirm viral fitness, but were not treated with antibody. In (A) two 2G12-treated (40 mg/kg) animals became infected: 90154 reached peak viremia of 2×107 on day 21 similar to controls; 95113 showed a one-week delay of infection onset and peak viremia was lower at 5×106. The remaining three 2G12-treated animals were protected against infection and showed no measurable viremia. In (B) all 4 control animals experienced peak viremia between 1×107 and 4×107 on day 21. The quantity of SIV viral RNA genomic copy equivalents (vRNA copy Eq/ml) in EDTA-anticoagulated plasma was determined using quantitative RT PCR [52]. The assay minimum detection is 150 copies of vRNA Eq/ml (2.1 log) with a 99% confidence level. The 2G12 antibody concentrations in the sera of the macaques at different time points were measured using three different ELISA formats. With a few exceptions, the determined serum concentrations derived from the three formats were generally in good agreement (Table 2). The first format used was an ELISA based on the ability of 2G12 to specifically recognize a relatively conserved cluster of oligomannose glycans. Serum was titrated against an immobilized synthesized oligomannose dendron conjugated to BSA [21]. The second ELISA format was based on a highly specific anti-idiotype-2G12 antibody (MIgG1 L13) that does not block the binding of gp120 or inhibit the neutralizing ability of 2G12 [23]. For comparison, a third ELISA format using monomeric gp120 JR-FL was used to measure the transferred 2G12 contained in the macaque serum. In all formats, a dilution series of serum was compared to a 2G12 standard curve and the concentration determined using a non-linear regression curve fit analysis. 10.1371/journal.ppat.1000433.t002 Table 2 2G12 serum antibody concentrations following passive administration. Protected Not Protected 95066 gp120 Man 4D anti-Id 95113 gp120 Man 4D anti-Id Day Serum Ab μ g/ml Day Serum Ab μg/ml −1 0 0 0 −1 0 0 0 0 1215 1241 911 0 822 1146 998 3 696 994 733 3 571 494 567 7 793 816 599 7 494 510 355 10 698 529 500 10 463 456 465 15 767 328 446 15 402 134 177 22 651 252 335 22 230 49 155 90140 gp120 Man 4D anti-Id 90154 gp120 Man 4D anti-Id Day Serum Ab μg/ml Day Serum Ab μg/ml −1 0 0 0 −1 0 0 0 0 954 901 1250 0 1252 1174 922 3 640 704 1005 3 750 575 545 7 694 545 838 7 597 371 448 10 640 398 705 10 554 221 348 15 453 452 502 15 453 174 328 22 173 394 505 22 231 101 342 1038 gp120 Man 4D anti-Id Day Serum Ab μg/ml −1 0 0 0 0 1203 921 891 3 959 527 717 7 927 413 714 10 898 365 690 15 605 220 496 22 296 103 147 The concentrations of transferred 2G12 in the serum of all experimental animals on the day of challenge (day 0) and during the following three weeks were determined by ELISA using three different formats. For each animal, the results from the different ELISA formats are shown in separate columns. The concentrations of 2G12 in the macaque sera were determined from the measurement of binding to monomeric JR-FL (gp120), to an immobilized synthetic oligomannose dendron conjugated to BSA [21] (Man4D), and to a highly specific anti-idiotype-2G12 antibody (MIgG1 L13) [23] (anti-id). In all formats, a dilution series of serum was compared to a 2G12 standard curve and the concentration determined using a nonlinear regression curve fit analysis performed in GraphPad Prism Software for Mac, Version 5.0a. The i.v. transfer of 2G12 at 40 mg/kg resulted in a high 2G12 serum concentration at the time of challenge that varied between 0.9 and 1.2 mg/ml. Table 3 summarizes the half-life of serum 2G12 that varied between 7.2 and 15.6 days in the 5 macaques. The average half-life of all animals as measured in the three ELISA formats is about 11 days (Table 3). The half-life of 2G12 in rhesus macaques has previously been noted as about 13 days [5]. 10.1371/journal.ppat.1000433.t003 Table 3 Half-life of transferred 2G12 in macaque serum. Half-life (days) of 2G12 in macaque serum Protected Not Protected Average (days) Animal 95066 90140 01038 95113 90154 gp120 ELISA 12.2 9.2 7.7 9.2 9.9 9.6 Man4D ELISA 10.7 15.6 7.2 10.5 8.4 10.5 L13 anti-idiotype ELISA 12.9 13.4 9.8 10.4 15.1 12.3 The data represents the half-life (t1/2) of serum 2G12 determined from data in three different ELISA formats over a period of three weeks following i.v. transfer of 40 mg/kg of 2G12. The half-life of transferred 2G12 ranged between 7.2 and 15.6 days in the 5 macaques with a somewhat shorter half-life observed in animal 01038. The average half-life of all animals as measured in the three ELISA formats is about 11 days. The half-life of 2G12 in rhesus macaques has previously been noted as about 13 days [5]. While for b12, 90% neutralization titers (IC90) of approximately 1∶80 in a PBMC assay were associated with protection in 50% of SHIVSF162P4 [8] and 90% of SHIVSF162P3 [9] challenged animals, a titer of only 1∶1 was sufficient to protect 60% of all animals with 2G12 in the experiment described here. It should be noted the IC90 for 2G12 neutralization of PBMC is approximately 900 µg/ml and the serum concentration of 2G12 at challenge was approximately 900–1,200 µg/ml making it impractical to directly measure neutralization in the PBMC assay. Using the generally more sensitive pseudovirus assay, 90% neutralization was not reached at a 1∶50 serum dilution for any of the 2G12-treated animals. Therefore, it does appear that MAb 2G12 can offer substantial protection at relatively low serum neutralizing titers. We next compared properties of 2G12 and b12 that might help explain the observed differences in protective activity relative to serum neutralization. One possibility is a gross difference in transudation efficiency for the two MAbs. For b12, it has been noted previously that the concentration of antibody at the vaginal surface following passive administration is only a small fraction of that in the serum [8]. If 2G12 was transudated to the vaginal surface much more efficiently than b12 then it is possible that it could achieve comparable neutralizing titers in vaginal fluids. This might lead to improved protection although it should be noted that no correlation between mucosal antibody levels and protection has been established. Earlier data suggests that 2G12 is not transudated unusually effectively although vaginal concentrations can vary widely [7]. We carried out a direct comparison of vaginal concentrations of b12 and 2G12 for a time period of 7 days after i.v. administration of 5 mg/kg MAb to 3 control macaques. As shown in Figure 2, the concentrations of the two MAbs transudated to the vaginal mucosal surface are similar and thus transudation is unlikely to contribute to protection differences between the MAbs. 10.1371/journal.ppat.1000433.g002 Figure 2 Comparison of b12 and 2G12 transudated to the vagina following intravenous administration. Each antibody treatment group consisted of three female Indian Rhesus macaques which were i.v.-administered 5 mg/kg of either b12 or 2G12 following Depo-provera treatment. Vaginal secretions from each animal were absorbed to cellulose wicks. A set of 3 samples per animal was taken at 6 hours, 12 hours, 24 hours, 4 days, and 7 days post i.v. antibody administration. The concentration of antibody in mucosal secretions was determined by ELISA from the clarified supernatant extracted from the wicks. Resulting data were compared to the corresponding antibody standard curve using nonlinear regression. Arithmetic means and standard deviations were calculated for each set of triplicate samples per animal. Data points were calculated from all animals at each timepoint and error bars represent the standard error of means. The typical time for viral challenge in protection experiments is indicated. The differences in the mean concentrations of b12 and 2G12 at each timepoint were evaluated in a student's t test and determined to be non-significant. Analyses performed in GraphPad Prism Software for Mac, Version 5.0a. Evidence has been obtained to suggest that interaction of b12 with Fc receptors can contribute to protection against SHIVSF162P3 challenge in macaques [9]. In particular, it was noted that wild-type b12 mediated antibody-dependent cell-mediated virus inhibition (ADCVI) and was effective at protection, whereas a b12 variant lacking FcR binding did not mediate ADCVI and was less effective than the wild-type antibody. If 2G12 were effective at ADCVI then this might contribute to enhanced protective activity. Figure 3 suggests that 2G12 is somewhat less effective than b12 at ADCVI on the basis of a strict concentration comparison. The significance of this result is discussed further below. 10.1371/journal.ppat.1000433.g003 Figure 3 Comparison of antibody-dependent cell-mediated viral inhibition (ADCVI) by 2G12 and b12. Target cells (CEM.NKR-CCR5) were infected with SHIVSF162P3 and incubated for 48 hours, washed to remove cell-free virus and combined with Rhesus PBMC effector cells and serially diluted antibody. Viral inhibition was measured after incubation for 7 days. 2G12 is somewhat less effective than b12 in mediating ADCVI for a strict concentration comparison. An unpaired Two-tailed t test (P = 0.3285) of b12 and 2G12 ADCVI with an F test comparison of variance reveals no significant difference (P = 0.4154). Analysis performed in GraphPad Prism Software for Mac, Version 5.0a. Since two MHC class I alleles (Mamu-B*08 and –B*17) have been associated with elite control of SIV replication, we evaluated all experimental animals by MHC genotyping (Table 4). We used PCR-SSP to test for a panel of 9 class Ι alleles previously shown to be important in SIV epitope presentation [24],[25],[26],[27],[28]. Protected animal 90140 was positive for Mamu-A*01, an allele that appears with high frequency in many colonies and has been associated with moderate reduction of SIVmac239 replication [27],[28],[29],[30]. Protected animal 95066 expresses the Mamu-B*01 allele. This allele remains on the panel based on early reports of SIV-derived epitopes [31],[32], but studies have shown that Mamu-B*01 does not bind SIV-derived epitopes and has no effect on SIV disease progression [26]. However, even with the presence of the Mamu-A*01 allele (which is not associated with elite control of SIV replication) in animal 90140, there is no apparent correlation with the allelic profiles of the animals in this study that would account for any unusual ability to resist infection. 10.1371/journal.ppat.1000433.t004 Table 4 MHC genotyping of macaques against MHC Class Ι alleles. Animal A01 A02 A08 A11 B01 B03 B04 B08 B17 95066P − − − − + − − − − 90140P + − − − − − − − − 01038P − − − − − − − − − 95113N − − − − − − − − − 90154N − − − − − − − − − 00071I − − − − − − − − − 01069I − − − − + − − − − 90126C − − − − + − − − − 00043C + − − − − − − − − Macaque samples were tested against a panel of nine MHC class Ι alleles that have previously been shown to be important in SIV epitope presentation or increased resistance to SIV infection [24],[25],[26]. The alleles are: Mamu-A*01, Mamu-A*02, Mamu-A*08, Mamu-A*11, Mamu-B*01, Mamu-B*03, Mamu-B*04, Mamu-B*08, and Mamu-B*17. Animal 90140 is positive for Mamu-A*01 and animal 95066 was determined to carry the Mamu-B*01 allele. Mamu-A*01 has been associated with moderate control of SIVmac239 replication [29],[30]. Mamu-B*01 remains on the panel based on early reports of SIV-derived epitopes [31],[32], but subsequent studies show that Mamu-B*01 does not bind SIV-derived epitopes and has no effect on SIV disease progression [26]. P, N, I, C denotes protected, non-protected, isotype control, and non-antibody treated control animals, respectively. Discussion The results presented here lend strong support to the notion that passively administered bNAb 2G12 is able to offer substantial protection against mucosal SHIV challenge at low serum neutralizing titers. In particular, 3 of 5 macaques showed sterilizing immunity on vaginal challenge with a high dose of SHIVSF162P3 when serum-neutralizing titers of 2G12 were of the order of 1∶1. This result contrasts strongly with protection observed with bNAb b12 when sterilizing immunity for the majority of animals is associated with neutralizing titers of very approximately 1∶100 and greater [8],[9],[33]. The result also contrasts with the quantitative studies of Martin and colleagues [10], which show sterilizing immunity against challenge with the ×4 SHIVDH12 only at high specific anti-DH12 antibody neutralizing titers. The result is however consistent with studies of Mascola and colleagues who showed that low serum neutralizing titers of 2G12 provided sterilizing immunity for 2 of 4 macaques vaginally challenged with the ×4 virus SHIV89.6P [7]. We investigated factors that might help explain the protective efficacy of 2G12, especially in relation to b12. This efficacy might be explained if 2G12 was transported very effectively to the site of infection; the results presented suggest this is not the case. We next noted that, although the neutralizing titers of 2G12 in our experiments are low, the actual serum concentrations of 2G12 are high since the antibody neutralizes the challenge virus SHIVSF162P3, and indeed other available SHIVs rather poorly. Therefore, it is possible that the protective efficacy of 2G12 derives from another anti-viral function of antibody that becomes important at high antibody concentration. One such function could be antibody-mediated host cell activity against SHIV-infected cells, which can be measured in the ADCVI assay. The results showed that 2G12 is somewhat less effective than b12 in the ADCVI assay. However, at the serum concentrations achieved in the passive transfer experiments, 2G12 should be able to promote infected cell killing in vivo. Therefore, one possible explanation for the differing relationship between neutralization and protection for b12 and 2G12 against SHIVSF162P challenge is that protection is determined not by neutralizing ability but solely by activity against infected cells. An argument against this explanation is provided by the observation that b12 can still provide substantial protection in the complete absence of Fc receptor function and ADCVI [9]. Furthermore, HIVIG, which tends to mediate effective ADCVI (GL and DNF, unpublished observations) is rather ineffective at protection against SHIV challenge [5],[7]. An explanation more concordant with the totality of data is that both neutralizing and extra-neutralizing activities are important for protection. Fc-mediated extra-neutralizing activities include, not only host cell activities against infected cells, but also those against free virions such as phagocytosis. It may be that 2G12 is able to compensate for its weak neutralization of SHIVSF162P3 by effective extra-neutralizing activities such as ADCVI. Protection studies using a SHIV that is sensitive to neutralization at low 2G12 concentrations but only sensitive to extra-neutralizing activities at high concentrations may help to better separate the contributions of different mechanisms to protection here. Alternatively, a 2G12 mutant lacking effector activity analogous to that generated for b12 [9] may help towards this aim. One intriguing difference between b12 and 2G12 that might relate to differences in protection has been described in terms of neutralization kinetics (PP, unpublished observations). Thus, it appears that following antibody-virus preincubation in vitro, neutralization by 2G12 occurs almost immediately while b12-mediated neutralization slowly progresses with time, reaching 2G12 neutralization levels only after hours. Only at high concentrations, corresponding to high neutralization titers, do b12 neutralization kinetics match those of 2G12. In vivo such kinetic differences may give 2G12 a noticeable advantage over b12: immediate efficiency at preventing target cell infection may be of particular importance in vivo as delay in neutralization may lead to cell infection and viral spread spinning out of antibody control. Further studies looking at correlations between antibody neutralization kinetics and protection efficiency should help reveal whether kinetics are important for protection. A further consideration for 2G12 is that the antibody recognizes high mannose glycans on the envelope gp120 surface. These glycans are also recognized by a number of lectins including DC-SIGN, which has been proposed to have a critical role in transmission by facilitating the transport of virus by dendritic cells to lymphoid tissues [34],[35]. Indeed, it has been shown that the addition of an N-glycan site to the V2 loop of SF162P leads to a gain of DC-SIGN binding and that this correlates with enhanced mucosal transmission of SHIVSF162P3 [36]. The gp120-DC-SIGN interaction can be perturbed by 2G12 but not b12 as shown in a number of assays including inhibition of whole virus binding to DC-SIGN-expressing cell lines [37],[38]. If the HIV-DC-SIGN interaction is critically important for the establishment of infection, then it is possible that 2G12 protection is mediated by inhibiting this interaction. Intriguingly, although 2G12 requires relatively high concentrations to neutralize SHIVSF162P3, presumably because of relatively low affinity for the envelope trimer [39],[40],[41], it binds with high nM affinity to monomeric gp120 from SHIVSF162P3. HIV is suggested to express both functional and other forms of envelope including monomeric envelope [42],[43]. If DC-SIGN was exploiting nonfunctional as well as or instead of functional envelope on virions then 2G12 might be an efficient competitor for binding to nonfunctional envelope at the in vivo concentrations achieved in our passive experiments. Future studies on the potential role of inhibiting the HIV-DC-SIGN interaction in blocking transmission could make use of anti-DC-SIGN antibodies. In summary, the data presented here, together with earlier data, show conclusively that monoclonal antibody 2G12 can offer protection against mucosal SHIV challenge at low neutralization titers. An explanation based on unusual 2G12 transudation properties to the mucosal surface is ruled out. Viable explanations include: (1) rapid 2G12 neutralization kinetics, (2) a critical role for extra-neutralizing, e.g. Fc-mediated, 2G12 activities under the conditions of the experiment, and (3) a critical role for 2G12 inhibition of virus interaction with lectin, e.g. DC-SIGN, bearing cells. Further in vivo protection studies will be required to distinguish these possibilities. Nevertheless, the results are provocative in suggesting the glycan shield as a potentially favorable HIV vaccine target. Materials and Methods Macaques All protocols for female Indian rhesus macaques were reviewed and approved by the Institutional Animal Care and Use Committees. The animals were housed in accordance with the American Association for Accreditation of Laboratory Animal Care Standards. At the start of the experiments, all animals were experimentally naïve and were negative for antibodies against HIV-1, SIV, and type D retrovirus. Virus challenge and i.v. antibody protocols are more fully described elsewhere [8],[44]. Challenge virus The virus used in this study was SHIVSF162P passage 3, which has been described elsewhere [45],[46],[47]. SHIVSF162P3 retains the R5 phenotype of HIV-1SF162. SHIVSF162P3, propagated in phytohemagglutin (PHA)-activated rhesus macaque peripheral blood mononuclear cells (PBMC), was obtained through the NIH AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH (Cat. No. 6526; Contributors: Drs. Janet Harouse, Cecilia Cheng-Mayer, and Ranajit Pal). Antibodies Recombinant 2G12 was obtained from Polymun Scientific, Vienna, Austria. The isotype control antibody DEN3, an anti-Dengue NS1 human IgG1 antibody was expressed in Chinese hamster ovary (CHO-K1) cells in glutamine-free custom formulated Glasgow minimum essential medium (GMEM Selection Media) (MediaTech Cellgro). For large-scale tissue culture, media was supplemented with 3.5% Ultra Low Bovine IgG Fetal Bovine Serum (Invitrogen) and grown in 10-layer Cellstacks and Cell Cubes (Corning). The antibody was purified using Protein A affinity matrix (GE Healthcare), and dialyzed against phosphate-buffered saline (PBS). Care was taken to minimize endotoxin contamination, which was monitored using a quantitative chromagenic Limulus Amoebecyte Lysate assay (Lonza) performed according to the manufacturer's recommendations. Antibody used for the passive transfer experiments contained <1 IU of endotoxin/mg. Plasma viral loads The quantity of SIV viral RNA genomic copy equivalents (vRNA copy Eq/ml) in EDTA-anticoagulated plasma was determined using a quantitative reverse-transcription PCR (QRT-PCR) assay. Briefly, vRNA was isolated from plasma using a GuSCN-based procedure as previously described [48]. QRT-PCR was performed using the SuperScript III Platinum One-Step Quantitative RT-PR System (Invitrogen, Carlsbad, CA). Reaction mixes did not contain bovine serum albumin (BSA). Reactions were run on a Roche Lightcycler 2.0 instrument and software. vRNA copy number was determined using LightCycler 4.0 software (Roche Molecular Diagnostics, Indianapolis, IN) to interpolate sample crossing points onto an internal standard curve prepared from 10-fold serial dilutions of a synthetic RNA transcript representing a conserved region of SIV gag. Serum antibody ELISAs 2G12 antibody concentrations in macaque sera were determined in ELISA by three different methods: (1) by binding to an immobilized synthetic oligomannose dendron [21] conjugated to BSA; (2) by binding to the anti-Idiotype 2G12 mouse IgG1 L13 kindly provided by Polymun Scientific, Vienna, Austria [49]; and (3) by binding to monomeric gp120 JR-FL kindly provided by Progenics [8]. Antibody concentrations in vaginal secretions The determination of antibody concentration in mucosal secretions was performed as described by Kozlowski, et al. [8],[50]. Briefly, vaginal secretions from each animal were absorbed to cellulose wicks (Solan Weck-Cel surgical spears; Xomed Surgical Products, Jacksonville, FL). A set of 3 samples per animal was taken at 6 hours, 12 hours, 24 hours, 4 days, and 7 days post i.v. antibody administration. Wicks were weighed before and after secretion absorption. Clarified supernatants extracted from the wicks were used to determine the concentration of antibody in mucosal secretions by ELISA. Resulting data was compared to the corresponding antibody standard curve using nonlinear regression. Arithmetic means and standard deviations were calculated for each set of triplicate samples per animal. The differences in the mean concentrations of b12 and 2G12 at each timepoint were evaluated in a student's t test. Analyses performed in GraphPad Prism Software for Mac, Version 5.0a. Neutralization assays Neutralization of antibodies and sera was assessed by 2 different methods. Neutralization of the primary isolate SHIVSF162P3, was performed using phytohemagglutinin (PHA)-activated peripheral blood mononuclear cells (PBMC) from a single rhesus macaque (no. 355) as target cells. Cells from this animal replicate SHIVSF162P efficiently. Neutralization assessment was carried out as described previously [8]. Neutralization titers of animal sera were reported by Monogram Biosciences, South San Francisco, CA after preparation of an HIV-1 envelope pseudotyped luciferase SHIVSF162P3 capable of single-round replication. The pseudovirus-based neutralization assay was performed as previously described [51]. MHC genotyping MHC genotyping by sequence-specific PCR was performed by the University of Wisconsin Genotyping Core with support of NIH grant 5R24RR16038-6 awarded to David I. Watkins and previously described [25]. Viral sequence amplification Viral RNA was extracted from 140 µl of monkey serum using the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer's instructions. 8 µl of this viral RNA was then used for cDNA synthesis using Superscript III (Invitrogen) primed by primer sf162rtn (5′-TTATAGCAAAATCCTTTCC-3′). 3 ul of the cDNA reaction was then used to amplify the gp160 open reading frame using primers sf162mf (5′–CACCATGAGAGTGAAGGGGATCAGGAAG-3′) and sf162rn (5′-TTATAGCAAAATCCTTTCCAAGCCCTGTC-3′) in combination with PfuUltra Hotstart DNA Polymerase from Stratagene. After an initial denaturation step at 95°C for 4 minutes, 35 cycles were performed with 95°C for 30 seconds, 52°C for 30 seconds and 72°C for 3 minutes, before a final elongation at 72°C for 10 minutes concluded the amplification. Sequences were determined after subcloning the PCR products into TOPO vectors. Statistics The experiment consisted of a total of 9 animals (n = 9) divided into treatment groups as follows: 2 animals (n = 2) in the isotope control group, 2 animals (n = 2) in the non-antibody-treated controls, and 5 animals (n = 5) in the 2G12-treated group. Statistical analyses were performed using Graph Pad Prism for Windows, version 5 (Graph Pad Software Inc., San Diego, CA, 2005). Protein Sequences GenBank accession locus for 2G12 is 10M3_H (heavy chain, Fab 2G12 unliganded) and 10M3_L (light chain, Fab 2G12 unliganded). GenBank accession locus for IgG1 b12 is AAB26306.
Author and article information
Contributors
: Role: Editor
Journal
Journal ID (nlm-ta): PLoS Pathog
Journal ID (iso-abbrev): PLoS Pathog
Journal ID (publisher-id): plos
Journal ID (pmc): plospath
Title:
PLoS Pathogens
Publisher:
Public Library of Science
(San Francisco, USA
)
ISSN
(Print):
1553-7366
ISSN
(Electronic):
1553-7374
Publication date Collection:
August
2012
Publication date
(Print):
August
2012
Publication date
(Electronic):
23
August
2012
Volume: 8
Issue: 8
Electronic Location Identifier: e1002890
Affiliations
[1
]Department of Microbiology and Immunobiology, Harvard Medical School, New England
Primate Research Center, Southborough, Massachusetts, United States of America
[2
]Immunology Division, Harvard Medical School, New England Primate Research Center,
Southborough, Massachusetts, United States of America
[3
]SAIC Frederick, National Cancer Institute at Frederick, Frederick, Maryland, United
States of America
[4
]Department of Pathology, Harvard Medical School, New England Primate Research Center,
Southborough, Massachusetts, United States of America
Author notes
* E-mail:
david_evans@
123456hms.harvard.edu
Article
Publisher ID: PPATHOGENS-D-12-00774DOI: 10.1371/journal.ppat.1002890
PMC ID: 3426556
PubMed ID: 22927823
SO-VID: 5bb25c77-6e05-419f-9b17-4372d247e002
Copyright statement:
Copyright @
2012
License:
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
History
Date
received
: 28
March
2012
Date
accepted
: 17
July
2012
Page count
Pages: 12
Funding
This work was supported by grants AI071306, AI063993, and RR000168/OD01103 from the
National Institutes of Health, and supplement 3P01AI071306-04S1 from the American
Recovery and Reinvestment Act (ARRA) of 2009, and in part with federal funds from
the National Cancer Institute, National Institutes of Health, under contract HHSN261200800001E.
DTE is an Elizabeth Glaser Scientist of the Elizabeth Glaser Pediatric AIDS Foundation.
The funders had no role in study design, data collection and analysis, decision to
publish, or preparation of the manuscript.
Categories
Subject:
Research Article
Subject:
Biology
Subject:
Immunology
Subject:
Microbiology
Subject:
Model Organisms
Subject:
Medicine
Subject:
Infectious Diseases
ScienceOpen disciplines: Infectious disease & Microbiology
Data availability:
ScienceOpen disciplines: Infectious disease & Microbiology