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      Metal-enhanced single-molecule fluorescence on silver particle monomer and dimer: coupling effect between metal particles.

      Nano Letters
      Base Sequence, Carbocyanines, Dimerization, Fluorescent Dyes, Metal Nanoparticles, Molecular Sequence Data, Oligonucleotides, Silver, Tiopronin

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          Abstract

          We prepared silver particle dimers with single Cy5 molecules localized between coupled metal particles. The silver particles with a 20 nm diameter were chemically bound with single-stranded oligonucleotides. The dimers were formed by hybridization with double-length single-stranded oligonucleotides that contained single Cy5 molecules. The image analysis revealed that the single-molecule fluorescence was enhanced 7-fold on the metal monomer and 13-fold on the metal dimer relative to the free Cy5-labeled oligonucleotide in the absence of metal. The lifetimes were shortened on the silver monomers and further shortened on the silver dimers, demonstrating the near-field interaction mechanism of fluorophore with the metal substrate. Finite-difference time-domain (FDTD) calculations were employed to study the distribution of electric field near the metal monomer and dimer. The coupling effect of metal particle on the fluorescence enhancement was discussed.

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          Author and article information

          Journal
          17580926
          2763908
          10.1021/nl071084d

          Chemistry
          Base Sequence,Carbocyanines,Dimerization,Fluorescent Dyes,Metal Nanoparticles,Molecular Sequence Data,Oligonucleotides,Silver,Tiopronin

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