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      Antioxidant Activity in Bee Products: A Review

      review-article
      * ,
      Antioxidants
      MDPI
      beeswax, honey, propolis, pollen, royal jelly, venom, antioxidant, polyphenols, honeybee, stingless bee

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          Abstract

          Bee products have been used since ancient times both for their nutritional value and for a broad spectrum of therapeutic purposes. They are deemed to be a potential source of natural antioxidants that can counteract the effects of oxidative stress underlying the pathogenesis of many diseases. In view of the growing interest in using bioactive substances from natural sources to promote health and reduce the risk of developing certain illnesses, this review aims to update the current state of knowledge on the antioxidant capacity of bee products such as honey, pollen, propolis, beeswax, royal jelly and bee venom, and on the analytical methods used. The complex, variable composition of these products and the multitude of analytical methods used to study their antioxidant activities are responsible for the wide range of results reported by a plethora of available studies. This suggests the need to establish standardized methods to more efficiently evaluate the intrinsic antioxidant characteristics of these products and make the data obtained more comparable.

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          Most cited references180

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          Antioxidant activity applying an improved ABTS radical cation decolorization assay

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            Use of a free radical method to evaluate antioxidant activity

            LWT - Food Science and Technology, 28(1), 25-30
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              The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay.

              A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing "antioxidant power." Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration. Absorbance changes are linear over a wide concentration range with antioxidant mixtures, including plasma, and with solutions containing one antioxidant in purified form. There is no apparent interaction between antioxidants. Measured stoichiometric factors of Trolox, alpha-tocopherol, ascorbic acid, and uric acid are all 2.0; that of bilirubin is 4.0. Activity of albumin is very low. Within- and between-run CVs are <1.0 and <3.0%, respectively, at 100-1000 micromol/liter. FRAP values of fresh plasma of healthy Chinese adults: 612-1634 micromol/liter (mean, 1017; SD, 206; n = 141). The FRAP assay is inexpensive, reagents are simple to prepare, results are highly reproducible, and the procedure is straightforward and speedy. The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.
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                Author and article information

                Journal
                Antioxidants (Basel)
                Antioxidants (Basel)
                antioxidants
                Antioxidants
                MDPI
                2076-3921
                07 January 2021
                January 2021
                : 10
                : 1
                : 71
                Affiliations
                Istituto Zooprofilattico Sperimentale delle Venezie, NRL for Honey Bee Health, 35020 Legnaro, Italy; fmutinelli@ 123456izsvenezie.it
                Author notes
                Author information
                https://orcid.org/0000-0001-9811-6308
                https://orcid.org/0000-0003-2903-9390
                Article
                antioxidants-10-00071
                10.3390/antiox10010071
                7827872
                33430511
                fc67a7e7-b487-4cbd-a0ea-d4c2370d102f
                © 2021 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 26 November 2020
                : 04 January 2021
                Categories
                Review

                beeswax,honey,propolis,pollen,royal jelly,venom,antioxidant,polyphenols,honeybee,stingless bee

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