The challenge of ovarian tissue culture: 2D versus 3D culture

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Abstract

Background

Cryopreservation of ovarian tissue is a powerful technique for preserving female fertility, as it can restore fertility and endocrine function. To increase the longevity of the transplant and decrease the risk of reimplantation of neoplastic cells, several studies have been carried out with culture of ovarian tissue. The aim of this study was to compare a conventional (2D) culture with an alginate matrix three-dimensional (3D) model for ovarian tissue culture.

Results

The ovarian tissue culture within the alginate matrix (3D) was similar to 2D culture, regarding follicular density and cell apoptosis in follicles and stroma. The proliferation rate remained stable in both models for follicles, but for stromal cell proliferation it decreased only in 3D culture ( p = 0.001). At 24 h of culture, cytotoxicity was lower in the 3D model ( p = 0.006). As culture time increased, cytotoxicity seemed similar. Degradation of the tissue was suggested by the histological score analysis of tissue morphology after 72 h of culture. Tissue injury was greater ( p = 0.01) in 3D culture due to higher interstitial oedema ( p = 0.017) and tissue necrosis ( p = 0.035).

Conclusion

According to our results, 3D culture of ovarian tissue has no advantage over 2Dculture; it is more time consuming and difficult to perform and has worse reproducibility.

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Most cited references 59

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Cancer statistics, 2020

Rebecca Siegel, Kimberly D Miller, Ahmedin Jemal (2020)

Each year, the American Cancer Society estimates the numbers of new cancer cases and deaths that will occur in the United States and compiles the most recent data on population-based cancer occurrence. Incidence data (through 2016) were collected by the Surveillance, Epidemiology, and End Results Program; the National Program of Cancer Registries; and the North American Association of Central Cancer Registries. Mortality data (through 2017) were collected by the National Center for Health Statistics. In 2020, 1,806,590 new cancer cases and 606,520 cancer deaths are projected to occur in the United States. The cancer death rate rose until 1991, then fell continuously through 2017, resulting in an overall decline of 29% that translates into an estimated 2.9 million fewer cancer deaths than would have occurred if peak rates had persisted. This progress is driven by long-term declines in death rates for the 4 leading cancers (lung, colorectal, breast, prostate); however, over the past decade (2008-2017), reductions slowed for female breast and colorectal cancers, and halted for prostate cancer. In contrast, declines accelerated for lung cancer, from 3% annually during 2008 through 2013 to 5% during 2013 through 2017 in men and from 2% to almost 4% in women, spurring the largest ever single-year drop in overall cancer mortality of 2.2% from 2016 to 2017. Yet lung cancer still caused more deaths in 2017 than breast, prostate, colorectal, and brain cancers combined. Recent mortality declines were also dramatic for melanoma of the skin in the wake of US Food and Drug Administration approval of new therapies for metastatic disease, escalating to 7% annually during 2013 through 2017 from 1% during 2006 through 2010 in men and women aged 50 to 64 years and from 2% to 3% in those aged 20 to 49 years; annual declines of 5% to 6% in individuals aged 65 years and older are particularly striking because rates in this age group were increasing prior to 2013. It is also notable that long-term rapid increases in liver cancer mortality have attenuated in women and stabilized in men. In summary, slowing momentum for some cancers amenable to early detection is juxtaposed with notable gains for other common cancers.

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Alginate: properties and biomedical applications.

Kuen Lee, David Mooney (2012)

Alginate is a biomaterial that has found numerous applications in biomedical science and engineering due to its favorable properties, including biocompatibility and ease of gelation. Alginate hydrogels have been particularly attractive in wound healing, drug delivery, and tissue engineering applications to date, as these gels retain structural similarity to the extracellular matrices in tissues and can be manipulated to play several critical roles. This review will provide a comprehensive overview of general properties of alginate and its hydrogels, their biomedical applications, and suggest new perspectives for future studies with these polymers.

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Caspases: the executioners of apoptosis.

Gerald Cohen (1997)

Apoptosis is a major form of cell death, characterized initially by a series of stereotypic morphological changes. In the nematode Caenorhabditis elegans, the gene ced-3 encodes a protein required for developmental cell death. Since the recognition that CED-3 has sequence identity with the mammalian cysteine protease interleukin-1 beta-converting enzyme (ICE), a family of at least 10 related cysteine proteases has been identified. These proteins are characterized by almost absolute specificity for aspartic acid in the P1 position. All the caspases (ICE-like proteases) contain a conserved QACXG (where X is R, Q or G) pentapeptide active-site motif. Capases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. The crystal structures of both caspase-1 and caspase-3 show that the active enzyme is a heterotetramer, containing two small and two large subunits. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates, including poly(ADP-ribose) polymerase and lamins, so precipitating the dramatic morphological changes of apoptosis. Apoptosis induced by CD95 (Fas/APO-1) and tumour necrosis factor activates caspase-8 (MACH/FLICE/Mch5), which contains an N-terminus with FADD (Fas-associating protein with death domain)-like death effector domains, so providing a direct link between cell death receptors and the caspases. The importance of caspase prodomains in the regulation of apoptosis is further highlighted by the recognition of adapter molecules, such as RAIDD [receptor-interacting protein (RIP)-associated ICH-1/CED-3-homologous protein with a death domain]/CRADD (caspase and RIP adapter with death domain), which binds to the prodomain of caspase-2 and recruits it to the signalling complex. Cells undergoing apoptosis following triggering of death receptors execute the death programme by activating a hierarchy of caspases, with caspase-8 and possibly caspase-10 being at or near the apex of this apoptotic cascade.

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Author and article information

Contributors

Ana Sofia Pais:

ORCID: http://orcid.org/0000-0002-1977-5258

asfpais@uc.pt

Journal

Journal ID (nlm-ta): J Ovarian Res

Journal ID (iso-abbrev): J Ovarian Res

Title: Journal of Ovarian Research

Publisher: BioMed Central (London )

ISSN (Electronic): 1757-2215

Publication date (Electronic): 1 November 2021

Publication date PMC-release: 1 November 2021

Publication date Collection: 2021

Volume: 14

Electronic Location Identifier: 147

Affiliations

[1 ]Reproductive Medicine Unit, Centro Hospitalar e Universitário de Coimbra (CHUC), E.P.E., Coimbra, Portugal

[2 ]GRID grid.8051.c, ISNI 0000 0000 9511 4342, Obstetrics Department, Faculty of Medicine, , University of Coimbra, ; Coimbra, Portugal

[3 ]GRID grid.8051.c, ISNI 0000 0000 9511 4342, University of Coimbra, Coimbra Institute for Clinical and Biomedical Research (iCBR) Area of Environment Genetics and Oncobiology (CIMAGO), Biophysics Institute of Faculty of Medicine, ; Coimbra, Portugal

[4 ]GRID grid.8051.c, ISNI 0000 0000 9511 4342, University of Coimbra, Center for Innovative Biomedicine and Biotechnology (CIBB), ; Coimbra, Portugal

[5 ]GRID grid.8051.c, ISNI 0000 0000 9511 4342, Clinical Academic Center of Coimbra (CACC), ; Coimbra, Portugal

[6 ]GRID grid.8051.c, ISNI 0000 0000 9511 4342, Center for Neuroscience and Cell Biology (CNC), CIBB, Azinhaga de Santa Comba, Celas, , University of Coimbra, ; Coimbra, Portugal

[7 ]GRID grid.8051.c, ISNI 0000 0000 9511 4342, PhD Programme in Experimental Biology and Biomedicine, , IIIUC - Institute for Interdisciplinary Research, University of Coimbra, ; Coimbra, Portugal

[8 ]Pathology Unit, Centro Hospitalar e Universitário de Coimbra (CHUC), E.P.E., Coimbra, Portugal

[9 ]GRID grid.8051.c, ISNI 0000 0000 9511 4342, University of Coimbra, Faculty of Medicine, ; Coimbra, Portugal

Author information

Ana Sofia Pais http://orcid.org/0000-0002-1977-5258

Article

Publisher ID: 892

DOI: 10.1186/s13048-021-00892-z

PMC ID: 8561954

PubMed ID: 34724957

SO-VID: f912b906-7d38-4bbc-bb5b-ba6053516c36

License:

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

History

Date received : 24 April 2021

Date accepted : 6 October 2021

Funding

Funded by: Centro Hospitalar e Universitário de Coimbra, Coimbra (CHUC)

Funded by: Sociedade Portuguesa de Ginecologia (SPG)

Funded by: Merck®

Funded by: National Funds via FCT (Foundation for Science and Technology)

Custom metadata

ScienceOpen disciplines: Obstetrics & Gynecology

Keywords: alginate,ovary,fertility preservation,tissue culture techniques

Data availability:

ScienceOpen disciplines: Obstetrics & Gynecology

Keywords: alginate, ovary, fertility preservation, tissue culture techniques

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The challenge of ovarian tissue culture: 2D versus 3D culture

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Abstract

Background

Results

Conclusion

Related collections

Bioscientifica Reproduction

Most cited references 59

Cancer statistics, 2020

Alginate: properties and biomedical applications.

Caspases: the executioners of apoptosis.

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Contributors

Journal

Affiliations

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Cited by 3