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Abstract
Emerging evidence documented the key functions of circular RNAs (circRNAs) in various
malignancies. Nevertheless, the research relevant to the clinical value and functions
of circRNAs in non-small cell lung cancer (NSCLC) is still very limited. In this study,
we performed qRT-PCR to assess the levels of hsa_circ_0007534 in NSCLC tissues and
cell lines and evaluated its clinical significance using Fisher's exact test, Kaplan-Meier
analysis and Cox regression analysis. Additionally, loss-of-function and gain-of-function
experiments were performed to detect whether hsa_circ_0007534 could affect cell proliferation,
apoptosis, metastatic properties and epithelial-mesenchymal transition (EMT) in A549
and H1299 cells. Further xenograft study was carried out to validate the in vitro
data. The results indicated that hsa_circ_0007534 was up-regulated in both NSCLC tissue
samples and cell lines and this up-regulation is linked to lymph node invasion and
advanced TNM stage. Hsa_circ_0007534 could also function as an unfavorable prognostic
indicator for the patients with NSCLC. For the part of functional assays, down-regulation
of hsa_circ_0007534 suppressed cell growth, migratory and invasive capacities; facilitating
cell apoptosis in A549 and H1299 cells. Conversely, up-regulation of hsa_circ_0007534
caused the opposite biological behaviors. What's more, animal experiments validated
the oncogenic role of hsa_circ_0007534. Ultimately, the present study indicates that
hsa_circ_0007534 might be a potential NSCLC-associated prognostic/therapeutic target.