EGFP transgene: a useful tool to track transplanted bone marrow mononuclear cell contribution to peripheral remyelination. – ScienceOpen
27
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      EGFP transgene: a useful tool to track transplanted bone marrow mononuclear cell contribution to peripheral remyelination.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Bone marrow mononuclear cells (BMMC) constitute a heterogeneous population with potential to promote tissue regeneration. For this reason, this cell fraction has recently become a therapeutic alternative to mesenchymal stem cells, as culture is not required and phenotypic transformations can be hence avoided. In this work, and in order to attain long-lasting cell labeling and study longer survival times, we used BMMC isolated from adult transgenic rats expressing GFP to reproduce our wild type model and evaluate their remyelination ability in a reversible model of Wallerian degeneration. RT-PCR and flow cytometry analysis confirmed that cells isolated from the transgenic strain exhibited similar expression levels of markers specific to multipotent progenitors (CD34, CD90 and CD105) and Schwann cells (MPZ, MBP, S100β and p75NTR) compared to wild type BMMC. BMMC expressing GFP retained their migration capacity, arriving exclusively at the injured nerve. Most importantly, and as detected through long-lasting cell tracking, some of these BMMC settled in the demyelinated area, mingled with endogenous cells, underwent phenotypic changes and colocalized with Schwann cell markers MBP and S100β. Also worth highlighting, transgenic BMMC replicated wild type BMMC effects in terms of MBP organization and levels. On the basis of these findings, BMMC isolated from transgenic animals constitute a useful tool to evaluate their role in peripheral nervous system demyelination-remyelination and the underlying mechanisms.

          Related collections

          Author and article information

          Journal
          Transgenic Res.
          Transgenic research
          Springer Nature
          1573-9368
          0962-8819
          April 2018
          : 27
          : 2
          Affiliations
          [1 ] CONICET, Instituto de Química y Fisicoquímica Biológicas Prof. Alejandro C. Paladini (IQUIFIB), Universidad de Buenos Aires, Buenos Aires, Argentina.
          [2 ] The Miami Project to Cure Paralysis and the Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, FL, USA.
          [3 ] CONICET, Instituto de Química y Fisicoquímica Biológicas Prof. Alejandro C. Paladini (IQUIFIB), Universidad de Buenos Aires, Buenos Aires, Argentina. setton@qb.ffyb.uba.ar.
          Article
          10.1007/s11248-018-0062-5
          10.1007/s11248-018-0062-5
          29453733
          de3c2ad8-1b8e-4bf0-8734-bb6e96ba9c28
          History

          Sciatic nerve injury,Bone marrow mononuclear cells,Remyelination,Transplantation

          Comments

          Comment on this article

          scite_
          0
          0
          0
          0
          Smart Citations
          0
          0
          0
          0
          Citing PublicationsSupportingMentioningContrasting
          View Citations

          See how this article has been cited at scite.ai

          scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

          Similar content362

          Cited by9