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      Isolation and Genetic Characterization of African Swine Fever Virus from Domestic Pig Farms in South Korea, 2019

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          Abstract

          On 17 September 2019, the first outbreak of African swine fever in a pig farm was confirmed in South Korea. By 9 October, 14 outbreaks of ASF in domestic pigs had been diagnosed in 4 cities/counties. We isolated viruses from all infected farms and performed genetic characterization. The phylogenetic analysis showed that all of fourteen ASFV isolates in South Korea belong to genotype II and serogroup 8. Additionally, all isolates had an intergenic region (IGR) II variant with additional tandem repeat sequences (TRSs) between the I73R and I329L genes and showed characteristics of central variable region (CVR) 1 of the B602L gene and IGR 1 of MGF 505 9R/10R genes. These are identical to the genetic characteristics of some European isolates and Chinese isolates.

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          Emergence of African Swine Fever in China, 2018

          Xintao Zhou, Nan Li, Yuzi Luo … (2018)
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            Genotyping field strains of African swine fever virus by partial p72 gene characterisation.

            J L Edrich, M-L Penrith, James G Thomson … (2003)
            A PCR-based sequencing method was developed which permits detection and characterization of African swine fever virus (ASFV) variants within 5 and 48 h, respectively, of receipt of a clinical specimen. Amplification of a 478 bp fragment corresponding to the C-terminal end of the p72 gene, confirms virus presence with genetic characterization being achieved by nucleotide sequence determination and phylogenetic analysis. The method was applied to 55 viruses including those representative of the major ASF lineages identified previously by restriction fragment length polymorphism (RFLP) analysis. Results confirmed that the p72 genotyping method identifies the same major viral groupings. Characterization of additional viruses of diverse geographical, species and temporal origin using the PCR-based method indicated the presence of ten major ASF genotypes on the African continent, the largest of which comprised a group of genetically homogeneous viruses recovered from outbreaks in Europe, South America, the Caribbean and West Africa (the ESAC-WA genotype). In contrast, viruses from southern and East African countries were heterogeneous, with multiple genotypes being present within individual countries. This study provides a rapid and accurate means of determining the genotype of field and outbreak strains of ASF and is therefore useful for molecular epidemiological clarification of ASF.
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              Genetic Variation among African Swine Fever Genotype II Viruses, Eastern and Central Europe

              Carmina Gallardo, Jovita Fernández-Pinero, Virginia Pelayo … (2014)
              African swine fever virus (ASFV) was first reported in eastern Europe/Eurasia in 2007. Continued spread of ASFV has placed central European countries at risk, and in 2014, ASFV was detected in Lithuania and Poland. Sequencing showed the isolates are identical to a 2013 ASFV from Belarus but differ from ASFV isolated in Georgia in 2007.
              Article 0 comments Cited 103 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Viruses
                Journal ID (iso-abbrev): Viruses
                Journal ID (publisher-id): viruses
                Title: Viruses
                Publisher: MDPI
                ISSN (Electronic): 1999-4915
                Publication date (Electronic): 30 October 2020
                Publication date Collection: November 2020
                Volume: 12
                Issue: 11
                Electronic Location Identifier: 1237
                Affiliations
                Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Korea; vetkhj@ 123456korea.kr (H.-J.K.); vet10@ 123456korea.kr (K.-H.C.); yjh562@ 123456naver.com (J.-H.R.); ckone0506@ 123456hanmail.net (M.-K.J.); cogkrud@ 123456naver.com (H.-G.C.); apjida@ 123456korea.kr (J.-D.C.); nahjj75@ 123456korea.kr (J.-J.N.); kyjvet@ 123456korea.kr (Y.-J.K.)
                Author notes
                [* ]Correspondence: kanghe@ 123456korea.kr ; Tel.: +82-54-912-0884
                Author information
                https://orcid.org/0000-0001-8021-8402
                https://orcid.org/0000-0002-9703-5909
                https://orcid.org/0000-0002-6013-484X
                Article
                Publisher ID: viruses-12-01237
                DOI: 10.3390/v12111237
                PMC ID: 7693868
                PubMed ID: 33143155
                SO-VID: dc74faf0-1acd-4b71-b7c5-50bcf7942661
                Copyright © © 2020 by the authors.
                License:

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                Date received : 07 October 2020
                Date accepted : 28 October 2020
                Categories
                Subject: Brief Report

                ScienceOpen disciplines: Microbiology & Virology
                Keywords: african swine fever,virus isolation,genetic characterization,south korea
                Data availability:
                ScienceOpen disciplines: Microbiology & Virology
                Keywords: african swine fever, virus isolation, genetic characterization, south korea

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