Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease

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Abstract

Background

Coronary artery disease (CAD), one of the most prevalent cardiovascular diseases, is a critical health issue that affects millions of individuals worldwide. It has been reported that miR-146b-5p exhibited a strong correlation with inflammatory responses and atherosclerosis. However, its association with the incidence and severity of CAD has not been substantiated in a large cohort. In the study, we focus on the expression of miR-146b-5p in peripheral blood mononuclear cells (PBMCs) of patients with CAD and preliminarily investigate its function and underlying mechanism.

Methods and results

The study encompassed a total of 452 participants, consisting 295 patients with CAD and 157 individuals without CAD. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) was performed to assess miR-146b-5p expression in PBMCs. We found that miR-146b-5p was significantly increased in PBMCs of patients with CAD compared with the control group. Binary logistic regression revealed that miR-146b-5p was associated with CAD. Receiver Operation Characteristic (ROC) analysis showed that the sensitivity and specificity of miR-146b-5p in discriminating CAD patients from non-CAD patients were meaningful. Subsequent subgroup analysis showed that miR-146b-5p was related to the severity of CAD. Furthermore, gain- and loss-of-function experiments in THP-1 cells showed that miR-146b-5p inhibited inflammation, cell proliferation, and migration. Mechanically, miR-146b-5p was involved in the classical NF-κB inflammatory pathway by directly targeting IKKβ.

Conclusion

Our study revealed that miR-146b-5p was higher in the PBMCs of CAD patients than non-CAD individuals, and established a correlation between miR-146b-5p and occurrence and severity of CAD. In addition, the inflammatory role of miR-146b-5p is mediated by targeting IKKβ.

Supplementary Information

The online version contains supplementary material available at 10.1186/s12872-025-04530-0.

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Most cited references 38

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Colchicine in Patients with Chronic Coronary Disease

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Evidence from a recent trial has shown that the antiinflammatory effects of colchicine reduce the risk of cardiovascular events in patients with recent myocardial infarction, but evidence of such a risk reduction in patients with chronic coronary disease is limited.

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The aim of this review is to summarize the incidence, prevalence, trend in mortality, and general prognosis of coronary heart disease (CHD) and a related condition, acute coronary syndrome (ACS). Although CHD mortality has gradually declined over the last decades in western countries, this condition still causes about one-third of all deaths in people older than 35 years. This evidence, along with the fact that mortality from CHD is expected to continue increasing in developing countries, illustrates the need for implementing effective primary prevention approaches worldwide and identifying risk groups and areas for possible improvement.

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NF-kappaB regulation in the immune system.

Qiutang Li, Inder Verma (2002)

The nuclear factor-kappaB (NF-kappaB)/REL family of transcription factors has a central role in coordinating the expression of a wide variety of genes that control immune responses. There has been intense scientific activity in the NF-kappaB field owing to the involvement of these factors in the activation and regulation of key molecules that are associated with diseases ranging from inflammation to cancer. In this review, we focus on our current understanding of NF-kappaB regulation and its role in the immune system and inflammatory diseases. We also discuss the role of NF-kappaB proteins as potential therapeutic targets in clinical applications.

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Author and article information

Contributors

Jiangtao Yan: jtyan@tjh.tjmu.edu.cn

Journal

Journal ID (nlm-ta): BMC Cardiovasc Disord

Journal ID (iso-abbrev): BMC Cardiovasc Disord

Title: BMC Cardiovascular Disorders

Publisher: BioMed Central (London )

ISSN (Electronic): 1471-2261

Publication date (Electronic): 5 February 2025

Publication date PMC-release: 5 February 2025

Publication date Collection: 2025

Volume: 25

Electronic Location Identifier: 81

Affiliations

[1 ]Department of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, ( https://ror.org/00p991c53) Wuhan, China

[2 ]Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders, Wuhan, China

Article

Publisher ID: 4530

DOI: 10.1186/s12872-025-04530-0

PMC ID: 11796014

SO-VID: d75f9fd7-3eb3-4e5c-8d3a-373c5f53f5c8

License:

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

History

Date received : 1 November 2024

Date accepted : 28 January 2025

Funding

Funded by: National Natural Science Foundation of China

Award ID: 82170392

Funded by: Hubei Key Research and Development Program

Award ID: 2021BCA121

Custom metadata

ScienceOpen disciplines: Cardiovascular Medicine

Keywords: mir-146b-5p,coronary artery disease,inflammation,ikkb

Data availability:

ScienceOpen disciplines: Cardiovascular Medicine

Keywords: mir-146b-5p, coronary artery disease, inflammation, ikkb

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Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease

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Abstract

Background

Methods and results

Conclusion

Supplementary Information

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Karger: Cardiovascular System

Most cited references 38

Colchicine in Patients with Chronic Coronary Disease

Epidemiology of coronary heart disease and acute coronary syndrome.

NF-kappaB regulation in the immune system.

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