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      “Evaluation of sealing ability of MM-MTA, Endosequence, and biodentine as furcation repair materials: UV spectrophotometric analysis”

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          Abstract

          Aim:

          To evaluate the sealing ability of MICRO-MEGA Mineral Trioxide Aggregate, Endosequence, Biodentine as furcation repair materials using a dye extraction leakage method.

          Materials and Methods:

          Forty mandibular molars were randomly divided according to the material used for perforation repair. Group I- (left unsealed) control, Group II-MICRO-MEGA Mineral Trioxide Aggregate, Group III - Endosequence, Group IV - Biodentine. All samples were subjected to orthograde and retrograde methylene blue dye challenge followed by dye extraction with 65% nitric acid. Samples were then analyzed using Ultra violet (UV) Visible Spectrophotometer.

          Results:

          Biodentine showed highest dye absorbance, whereas Endosequence showed lowest dye absorbance when compared with other repair materials.

          Conclusion:

          Within the limitations of this study, it was observed that Endosequence showed better sealing ability when compared with other root repair materials.

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          Biocompatibility of two novel root repair materials.

          Markus Haapasalo, Ya Shen, J.-J. Ma … (2011)
          The purpose of the present study was to evaluate the biocompatibility of 2 root-end filling materials, Endosequence Root Repair Material Putty (ERRM Putty) and Paste (ERRM Paste) and compare them with gray mineral trioxide aggregate (MTA). ERRM Putty, ERRM Paste, MTA, intermediate restorative material (IRM), and Cavit G were tested. For cytotoxicity assay, human gingival fibroblasts were incubated for 1, 3, and 7 days with extracts of varying concentrations from materials set for 2 days or 7 days. Cell viability was evaluated by methyl-thiazol-tetrazolium (MTT) assay. For cell adhesion assay, materials set for 7 days were examined under scanning electron microscope directly after setting, after incubation in cell culture medium for 7 days, and after incubation in gingival fibroblast suspension at a density of 5 × 10(4) cells/well for 2 and 7 days. The constituents of crystals formed on surface of materials were determined by energy dispersive analysis by x-ray. Cell viability was significantly correlated with the type of material, setting time, and incubation time (P < .001 for all parameters). ERRM Putty and ERRM Paste displayed similar cell viabilities to MTA at all experimental conditions, except that fresh samples of ERRM Paste showed slightly lower cell viabilities than MTA. Cell viabilities with IRM and Cavit G were significantly lower than with the other 3 materials (P < .001). Similar surface crystallographic features and cell adhesion were observed on ERRM Paste, ERRM Putty, and MTA. ERRM Putty and ERRM Paste displayed similar in vitro biocompatibility to MTA. Copyright © 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
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            Antibacterial activity of endosequence root repair material and proroot MTA against clinical isolates of Enterococcus faecalis.

            Robert Sedgley, Karen Lovato (2011)
            Endodontic repair materials such as mineral trioxide aggregate (MTA) are used for various endodontic procedures. An alternative material to MTA with purportedly improved handling properties is EndoSequence Root Repair Material, which is available as premixed putty (ESP) or syringeable paste (ESS) and is described as possessing antibacterial activity during its setting reaction due to its highly alkaline pH. The aim of this in vitro study was to determine whether ESP and ESS possess antibacterial properties against a collection of Enterococcus faecalis strains recovered from root canal infections. The hypotheses tested were that (1) ESP and ESS possess antibacterial activity during their setting reaction, (2) there is no difference between ESP, ESS, and MTA in antibacterial activity, and (3) E. faecalis strains isolated from root canals differ in susceptibility to the materials. The direct contact test was used. ESP, ESS, and white MTA were preincubated at 37°C in >95% humidity for 30 minutes and 24 hours before 1-hour direct contact exposure to E. faecalis strains (n = 10). Absence of antibacterial carryover effect from the materials to the bacterial cultures was confirmed. Log(10) viable counts were compared by using analysis of variance with significance level at P ≤ .05. Combining data for all strains, the mean (± standard deviation) log(10) viable counts for ESP (4.55 ± 0.85), ESS (4.5 ± 0.95), and MTA (4.12 ± 1.26) were significantly lower than for untreated controls (7.40 ± 0.33) (P < .0001). The reduction in viable counts ranged from 1.86 ± 0.24 to 4.78 ± 0.42, with no statistically significant differences between the materials or preincubation periods. One strain was significantly more susceptible than 4 other strains. ESP, ESS, and MTA had similar antibacterial efficacy against clinical strains of E. faecalis. Clinical strains varied in their susceptibility to the root repair materials. Copyright © 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
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              Comparative evaluation of push-out bond strength of ProRoot MTA, Biodentine, and MTA Plus in furcation perforation repair

              Vivek Aggarwal, Mamta Singla, Sanjay Miglani … (2013)
              Purpose: Few studies have comparatively evaluated the push-out bond strength of different calcium silicate–based materials (CSMs) used in furcal perforation repair. The objective of this in vitro study was to comparatively evaluate the push-out bond strength of commercially available CSMs used as furcation repair materials, in the presence of blood contamination. Materials and Methods: Furcal perforations were made in 120 molars and were divided on the basis of the repair material used (ProRoot MTA, Biodentine, and MTA Plus), blood contamination, and duration of setting time (24 h vs. 7 days). Push-out bond strength was measured and analyzed by three-way analysis of variance (ANOVA) test. Results: Push-out bond strength increased with time. The 24-h push-out strength of MTA was less than that of Biodentine. Blood contamination affected the push-out bond strength of MTA Plus irrespective of the setting time. Conclusion: Caution should be taken while condensing restorative materials over furcation repair materials.
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                Author and article information

                Journal
                Journal ID (nlm-ta): J Conserv Dent
                Journal ID (iso-abbrev): J Conserv Dent
                Journal ID (publisher-id): JCD
                Title: Journal of Conservative Dentistry : JCD
                Publisher: Medknow Publications & Media Pvt Ltd (India )
                ISSN (Print): 0972-0707
                ISSN (Electronic): 0974-5203
                Publication date (Print): Jul-Aug 2014
                Volume: 17
                Issue: 4
                Pages: 340-343
                Affiliations
                [1]Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences, Takkellapadu, Guntur, Andhra Pradesh, India
                Author notes
                Address for correspondence: Dr. E. Jeevani, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences, Takkellapadu, Guntur - 522 509, Andhra Pradesh, India. E-mail: jeevanieppala12@ 123456gmail.com
                Article
                Publisher ID: JCD-17-340
                DOI: 10.4103/0972-0707.136449
                PMC ID: 4127692
                PubMed ID: 25125846
                SO-VID: ce90cfc7-a1fc-4902-b149-0e3d66002748
                Copyright © Copyright: © Journal of Conservative Dentistry
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 02 February 2014
                Date revision received : 20 April 2014
                Date accepted : 27 April 2014
                Categories
                Subject: Original Article

                ScienceOpen disciplines: Dentistry
                Keywords: dye extraction,furcation repair,perforation,sealing ability
                Data availability:
                ScienceOpen disciplines: Dentistry
                Keywords: dye extraction, furcation repair, perforation, sealing ability

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