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      Comparison of sealing ability of ProRoot MTA, RetroMTA, and Biodentine as furcation repair materials: An ultraviolet spectrophotometric analysis

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          Abstract

          Aim:

          To evaluate the sealing ability of ProRooT MTA, RetroMTA, and Biodentine as furcation repair materials using dye extraction leakage method.

          Materials and Methods:

          Thirty-five mandibular molars were randomly divided into four groups according to the material used for perforation repair. Group I — ProRoot MTA (10 samples), Group II — RetroMTA (10 samples), Group III — Biodentine (10 samples), and Group IV (Control) — left unrepaired (5 samples). All samples were subjected to orthograde and retrograde Methylene blue dye challenge followed by dye extraction with concentration 65% nitric acid. Samples were then analyzed using ultraviolet-visible spectrophotometer using 550 nm wave lengths.

          Statistical Analysis:

          One-way analysis of variance, Tukey-Kramer multiple comparisons test.

          Results:

          Biodentine showed least dye absorbance while RetroMTA showed highest dye absorbance values when compared with other repair materials.

          Conclusion:

          Within the limitations of this study, it was observed that Biodentine showed better sealing ability when compared with other root repair materials.

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          Most cited references17

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          Biomineralization ability and interaction of mineral trioxide aggregate and white portland cement with dentin in a phosphate-containing fluid.

          Mineral trioxide aggregate (MTA) has been shown to be bioactive because of its ability to produce biologically compatible carbonated apatite. This study analyzed the interaction of MTA and white Portland cement with dentin after immersion in phosphate-buffered saline (PBS). Dentin disks with standardized cavities were filled with ProRoot MTA, MTA Branco, MTA BIO, white Portland cement + 20% bismuth oxide (PC1), or PC1 + 10% of calcium chloride (PC2) and immersed in 15 mL of PBS for 2 months. The precipitates were weighed and analyzed by scanning electron microscopy (SEM) and x-ray diffraction. The calcium ion release and pH of the solutions were monitored at 5, 15, 25, and 35 days. The samples were processed for SEM observations. Data were analyzed by using analysis of variance or Kruskall-Wallis tests. Our findings revealed the presence of amorphous calcium phosphate precipitates with different morphologies. The apatite formed by the cement-PBS system was deposited within collagen fibrils, promoting controlled mineral nucleation on dentin, observed as the formation of an interfacial layer with tag-like structures. All the cements tested were bioactive. The cements release some of their components in PBS, triggering the initial precipitation of amorphous calcium phosphates, which act as precursors during the formation of carbonated apatite. This spontaneous precipitation promotes a biomineralization process that leads to the formation of an interfacial layer with tag-like structures at the cement-dentin interface.
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            Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine.

            To compare Biodentine and White ProRoot mineral trioxide aggregate (MTA) with regard to Ca and Si uptake by adjacent root canal dentine in the presence of phosphate-buffered saline (PBS). Root canals of bovine incisor root segments were instrumented, filled with either Biodentine or MTA (n = 20 each) and then immersed in Ca-and Mg-free PBS for 1, 7, 30 or 90 days (n = 5 each). Unfilled, unimmersed dentine specimens (n = 5) served as controls. The specimens were sectioned longitudinally, and the ultrastructure of the dentine-material interface and the elemental composition/distribution in the material-adjacent dentine were analysed using a wavelength-dispersive X-ray spectroscopy electron probe microanalyser with image observation function. Data were statistically analyzed using one-way anova and Tukey's honestly significant difference test or the Mann-Whitney U-test. Along the material-dentine interface, both materials formed a tag-like structure that was composed of either Ca- and P-rich crystalline deposits or the material itself. The width of a Ca- and Si-rich layer detected along the dentine layer of the material-dentine interface showed increases over time. The Ca- and Si-rich layer width was significantly larger (P < 0.05) in Biodentine than MTA at 30 and 90 days. Both Biodentine and MTA caused the uptake of Ca and Si in the adjacent root canal dentine in the presence of PBS. The dentine element uptake was more prominent for Biodentine than MTA. © 2011 International Endodontic Journal.
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              Biodentine™ material characteristics and clinical applications: a review of the literature.

              Biodentine™ is a new version of calcium silicate-based inorganic cement. The aim of this review is to provide a detailed analysis of the physical and biological properties of Biodentine™ and to compare these properties with those of other tricalcium silicate cements viz. mineral trioxide aggregate (MTA) and Bioaggregate™ (Bioaggregate). A comprehensive systematic literature search for all publications to date was performed on 20th November 2013 by two independent reviewers in Medline (PubMed), Embase, Web of Science, CENTRAL (Cochrane), SIGLE, SciELO, Scopus, Lilacs and clinicaltrials.gov using the search terms Biodentine, "tricalcium silicate", Ca3SiO5, "dentine substitute", "dentin substitute" and RD 94. In addition to the electronic search, hand searches and reference searches were performed to include articles published in journals that were not indexed in Medline. Randomised control trials (RCT), case control studies, case series, case reports, in vitro studies, animal studies and short communications in English language were considered for this review. Considering the superior physical and biologic properties, Biodentine™ could be an efficient alternative to MTA to be used in a variety of clinical applications. There appears to be a wide range of clinical applications where Biodentine™ could be used in the field of endodontics, dental traumatology, restorative dentistry and pediatric dentistry. Although it seems to be good clinical practice, currently there is little clinical evidence to support all potential indications.
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                Author and article information

                Journal
                J Conserv Dent
                J Conserv Dent
                JCD
                Journal of Conservative Dentistry : JCD
                Medknow Publications & Media Pvt Ltd (India )
                0972-0707
                0974-5203
                Nov-Dec 2015
                : 18
                : 6
                : 445-448
                Affiliations
                [1]Department of Conservative Dentistry and Endodontics, Swargiya Dadasaheb Kalmegh Smruti Dental College and Hospital, Hingna, Nagpur, Maharashtra, India
                Author notes
                Address for correspondence: Dr. Roshan Chandrakant Sinkar, Plot No 25, Shraddha, Yogayog Society, Behind Rahul Nagar, Ambarnath (East), Thane - 421 501, Maharashtra, India. E-mail: roshansinkar@ 123456yahoo.in
                Article
                JCD-18-445
                10.4103/0972-0707.168803
                4693315
                26752836
                e111983f-4d9c-4846-ba16-60ad3083466b
                Copyright: © 2015 Journal of Conservative Dentistry

                This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

                History
                : 15 June 2015
                : 14 August 2015
                : 08 September 2015
                Categories
                Original Article

                Dentistry
                dye-extraction,furcation repair,perforation,ultraviolet spectrophotometer
                Dentistry
                dye-extraction, furcation repair, perforation, ultraviolet spectrophotometer

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