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      Hsa_circ_0012563 promotes migration and invasion of esophageal squamous cell carcinoma by regulating XRCC1/EMT pathway

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          Abstract

          Background

          Recent reports have indicated that circular RNA (circRNA) may regulate tumorigenesis development. However, the function of circRNAs in esophageal squamous cell carcinoma (ESCC) is unclear.

          Material and method

          The RT‐qPCR assay was performed to detect hsa_circ_0012563 expression in ESCC tissues and cell lines. Then, the MTT assay, colony formation assay, flow cytometric assay, and cell migration and invasion assay were performed to examine the function of hsa_circ_0012563. In addition, the RT‐PCR and Western blot were used to detect XRCC1 and epithelial‐to‐mesenchymal transition (EMT) related gene expression.

          Results

          The RT‐qPCR revealed that the hsa_circ_0012563 expression was remarkably upregulated in ESCC tissue and ESCC cell lines. Functionally, downregulation of hsa_circ_0012563 suppressed cell proliferation, migration, and invasion and promoted cell apoptosis. Mechanically, the knockdown of hsa_circ_0012563 inhibited XRCC1‐mediated EMT pathway to suppress cell migration and invasion.

          Conclusions

          Therefore, these results reveal hsa_circ_0012563 is a critical oncogene and may be a novel biomarker in ESCC.

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          Circular RNAs: splicing's enigma variations.

          Matthias W Hentze, Thomas Preiss (2013)
          Article 0 comments Cited 242 times – based on 0 reviews     Review now
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            Circular RNA has_circ_0067934 is upregulated in esophageal squamous cell carcinoma and promoted proliferation

            Wenjia Xia, Mantang Qiu, Rui-rui Chen … (2016)
            Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent and deadly types of cancer worldwide especially in Eastern Asia and the prognosis of ESCC remain poor. Recent evidence suggests that circular RNAs (circRNAs) play important roles in multiple diseases, including cancer. In this study, we characterized a novel circRNA termed hsa_circ_0067934 in ESCC tumor tissues and cell lines. We analyzed a cohort of 51 patients and found that hsa_circ_0067934 was significantly overexpressed in ESCC tissues compared with paired adjacent normal tissues. The high expression level of hsa_circ_0067934 was associated with poor differentiation (P = 0.025), I-II T stage (P = 0.04), and I-II TNM stage (P = 0.021). The in vitro silence of hsa_circ_0067934 by siRNA inhibited the proliferation and migration of ESCC cells and blocked cell cycle progression. Cell fraction analyses and fluorescence in situ hybridization detected that hsa_circ_0067934 was mostly located in the cytoplasm. Our findings suggest that hsa_circ_0067934 is upregulated in ESCC tumor tissue. Our data suggest that hsa_circ_0067934 represents a novel potential biomarker and therapeutic target of ESCC.
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              RNA sequencing reveals the expression profiles of circRNA and indicates that circDDX17 acts as a tumor suppressor in colorectal cancer

              Xiang-Nan Li, Zhen-Jun Wang, Chun-Xiang Ye … (2018)
              Background Circular RNA (circRNA) is a novel class of noncoding RNAs with functions in various pathophysiological activities. However, the expression profiles and functions of circRNAs in colorectal cancer (CRC) remain largely unknown. Methods High-throughput RNA sequencing (RNA-seq) was performed to assess circRNA expression profiles in 4 paired CRC tissues, and significantly dysregulated circRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to predict the potential functions of dysregulated circRNAs. Target miRNAs of circRNAs were predicted using miRanda software, and were further analyzed combining DIANA-miRPath v.3 platform (Reverse Search module) with KEGG pathways of COLORECTAL CANCER and MicroRNAs in cancer (Entry: map05210 and map05206). CircRNA-miRNA interaction networks were constructed using Cytoscape software. Expression levels of a significantly down-regulated circRNA, circDDX17 (hsa_circ_0002211), was detected by qRT-PCR in 60 paired CRC tissues. CircDDX17 was knockdown by siRNA, and the biological functions of circDDX17 were examined in CRC cell lines. Results Totally 448 differentially expressed circRNAs were identified, including 394 up-regulated and 54 down-regulated circRNAs. qRT-PCR validation confirmed the reliability of the RNA-Seq data. GO and KEGG analyses revealed that these dysregulated circRNAs were potentially implicated in CRC pathogenesis. Analyses by combining miRanda and miRPath softwares with KEGG pathways suggested that the miRNAs targeted by the top 10 dysregulated circRNAs were associated with the KEGG pathways of COLORECTAL CANCER and MicroRNAs in cancer, indicating that circRNA-miRNA interactions might play important functional roles in the initiation and progression of CRC. The results of qRT-PCR for circDDX17 in 60 paired CRC tissues showed that circDDX17 was significantly down-regulated in CRC tissues and associated with unfavorable clinicopathological parameters. In vitro experiments showed that silencing of circDDX17 promoted CRC cell proliferation, migration, invasion, and inhibited apoptosis. Conclusions In conclusion, we have identified numerous circRNAs that are dysregulated in CRC tissues compared with adjacent normal mucosa tissues. Bioinformatic analyses suggested that these dysregulated circRNAs might play important functional roles in CRC tumorigenesis. CircDDX17 functions as a tumor suppressor and could serve as a potential biomarker and a therapeutic target for CRC. Electronic supplementary material The online version of this article (10.1186/s13046-018-1006-x) contains supplementary material, which is available to authorized users.
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                Author and article information

                Contributors
                Yongming Han:
                ORCID: https://orcid.org/0000-0002-1603-5051
                hanym575@163.com
                Journal
                Journal ID (nlm-ta): J Clin Lab Anal
                Journal ID (iso-abbrev): J. Clin. Lab. Anal
                Journal ID (doi): 10.1002/(ISSN)1098-2825
                Journal ID (publisher-id): JCLA
                Title: Journal of Clinical Laboratory Analysis
                Publisher: John Wiley and Sons Inc. (Hoboken )
                ISSN (Print): 0887-8013
                ISSN (Electronic): 1098-2825
                Publication date (Electronic): 17 March 2020
                Publication date Collection: August 2020
                Volume: 34
                Issue: 8 ( doiID: 10.1002/jcla.v34.8 )
                Electronic Location Identifier: e23308
                Affiliations
                [ 1 ] Department of Thoracic Surgery Tongren Hospital of Wuhan University (Wuhan Third Hospital) Wuhan China
                [ 2 ] Department of Pharmaceutical Sciences Tongren Hospital of Wuhan University (Wuhan Third Hospital) Wuhan China
                [ 3 ] School of Basic Medicine Hubei University of Chinese Medicine Wuhan China
                Author notes
                [*] [* ] Correspondence

                Yongming Han, School of Basic Medicine, Hubei University of Chinese Medicine. No. 1 Huangjiahu West Road, Wuhan, Hubei 430065, China.

                Email: hanym575@ 123456163.com

                Author information
                https://orcid.org/0000-0002-1603-5051
                Article
                Publisher ID: JCLA23308
                DOI: 10.1002/jcla.23308
                PMC ID: 7439410
                PubMed ID: 32185826
                SO-VID: c930d8ed-9aa8-4f18-a8c2-b7e85eb5ca9a
                Copyright © © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc.
                License:

                This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 13 February 2020
                Date revision received : 02 March 2020
                Date accepted : 03 March 2020
                Page count
                Figures: 4, Tables: 2, Pages: 7, Words: 3575
                Categories
                Subject: Research Article
                Subject: Research Articles
                Custom metadata
                source-schema-version-number 2.0
                cover-date August 2020
                details-of-publishers-convertor Converter:WILEY_ML3GV2_TO_JATSPMC version:5.8.7 mode:remove_FC converted:20.08.2020

                ScienceOpen disciplines: Clinical chemistry
                Keywords: emt,escc,hsa_circ_0012563,invasion,migration,xrcc1
                Data availability:
                ScienceOpen disciplines: Clinical chemistry
                Keywords: emt, escc, hsa_circ_0012563, invasion, migration, xrcc1

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