Activation of AMPA/Kainate Receptors but Not Acetylcholine Receptors Causes Mg ²⁺ Influx into Retzius Neurones of the Leech Hirudo medicinalis

Abstract

In Retzius neurones of the medicinal leech, Hirudo medicinalis, kainate activates ionotropic glutamate receptors classified as AMPA/kainate receptors. Activation of the AMPA/kainate receptor–coupled cation channels evokes a marked depolarization, intracellular acidification, and increases in the intracellular concentrations of Na ⁺ ([Na ⁺] _i) and Ca ²⁺. Qualitatively similar changes are observed upon the application of carbachol, an activator of acetylcholine receptor-coupled cation channels. Using multibarrelled ion-selective microelectrodes it was demonstrated that kainate, but not carbachol, caused additional increases in the intracellular free Mg ²⁺ concentration ([Mg ²⁺] _i). Experiments were designed to investigate whether this kainate-induced [Mg ²⁺] _i increase was due to a direct Mg ²⁺ influx through the AMPA/kainate receptor–coupled cation channels or a secondary effect due to the depolarization or the ionic changes. It was found that: (a) Similar [Mg ²⁺] _i increases were evoked by the application of glutamate or aspartate. (b) All kainate-induced effects were inhibited by the glutamatergic antagonist DNQX. (c) The magnitude of the [Mg ²⁺] _i increases depended on the extracellular Mg ²⁺ concentration. (d) A reduction of the extracellular Ca ²⁺ concentration increased kainate-induced [Mg ²⁺] _i increases, excluding possible Ca ²⁺ interference at the Mg ²⁺-selective microelectrode or at intracellular buffer sites. (e) Neither depolarizations evoked by the application of 30 mM K ⁺, nor [Na ⁺] _i increases induced by the inhibition of the Na ⁺/K ⁺ ATPase caused comparable [Mg ²⁺] _i increases. (f) Inhibitors of voltage-dependent Ca ²⁺ channels did not affect the kainate-induced [Mg ²⁺] _i increases. Moreover, previous experiments had already shown that intracellular acidification evoked by the application of 20 mM propionate did not cause changes in [Mg ²⁺] _i. The results indicate that kainate-induced [Mg ²⁺] _i increases in leech Retzius neurones are due to an influx of extracellular Mg ²⁺ through the AMPA/kainate receptor–coupled cation channel. Mg ²⁺ may thus act as an intracellular signal to distinguish between glutamatergic and cholinergic activation of leech Retzius neurones.