The change in metabolic activity of a large benthic foraminifera as a function of light supply

Abstract

We studied metabolic activity of the symbiont-bearing large benthic foraminifer Heterostegina depressa under different light conditions. Besides the overall photosynthetic performance of the photosymbionts estimated by means of variable fluorescence, the isotope uptake ( ¹³C and ¹⁵N) of the specimens (= holobionts) was measured. Heterostegina depressa was either incubated in darkness over a period of 15 days or exposed to an 16:8 h light:dark cycle mimicking natural light conditions. We found photosynthetic performance to be highly related to light supply. The photosymbionts, however, survived prolonged darkness and could be reactivated after 15 days of darkness. The same pattern was found in the isotope uptake of the holobionts. Based on these results, we propose that ¹³C-carbonate and ¹⁵N-nitrate assimilation is mainly controlled by the photosymbionts, whereas ¹⁵N-ammonium and ¹³C-glucose utilization is regulated by both, the symbiont and the host cells.