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Abstract
Rice stripe disease, caused by Rice stripe virus (RSV), may lead to severe or even
crippling losses in many rice-cultured countries and regions. As the most important
vector of RSV, the small brown planthopper (SBPH) (Laodelphax striatellus) is largely
responsible for the epidemic phase of the disease. Therefore, a rapid identification
of RSV in the SBPH is of a great need for disease forecasting. A reverse transcription
polymerase chain reaction (RT-PCR) assay is described to amplify a RSV gene in individual
L. striatellus. By using primers matched to the viral RNA dependent RNA polymerase
gene in RNA1, a 445 bp product was detected in viruliferous SBPHs. Meanwhile, the
PCR products produced by the SBPH actin primers constructed across the boundary of
an intron and an exon were used as RNA specific positive control for each stage of
the experiment to ensure the validity of the negative results. Duplex RT-PCR conditions
were established for the simultaneous detection of RSV and actin. This approach can
be used for the early detection of RSV in L. striatellus and the subsequent rice stripe
disease forecasting.