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      SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor

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          Abstract

          A specific aptameric sequence has been immobilized on short polyethyleneglycol (PEG) interface on gold nano-film deposited on a D-shaped plastic optical fiber (POFs) probe, and the protein binding has been monitored exploiting the very sensitive surface plasmon resonance (SPR) phenomenon. The receptor-binding domain (RBD) of the SARS-CoV-2 spike glycoprotein has been specifically used to develop an aptasensor. Surface analysis techniques coupled to fluorescence microscopy and plasmonic analysis have been utilized to characterize the biointerface. Spanning a wide protein range (25÷1000 nM), the SARS-Cov-2 spike protein was detected with a Limit of Detection (LoD) of about 37 nM. Different interferents (BSA, AH1N1 hemagglutinin protein and MERS spike protein) have been tested confirming the specificity of our aptasensor. Finally, a preliminary test in diluted human serum encouraged its application in a point-of-care device, since POF-based aptasensor represent a potentially low-cost compact biosensor, characterized by a rapid response, a small size and could be an ideal laboratory portable diagnostic tool.

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          NIH Image to ImageJ: 25 years of image analysis

          Kevin W Eliceiri, Caroline A Schneider, Wayne S Rasband (2019)
          For the past twenty five years the NIH family of imaging software, NIH Image and ImageJ have been pioneers as open tools for scientific image analysis. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.
          Article 0 comments Cited 9044 times – based on 0 reviews     Review now
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            Diagnosing COVID-19: The Disease and Tools for Detection

            Buddhisha Udugama, Pranav Kadhiresan, Hannah Kozlowski … (2020)
            COVID-19 has spread globally since its discovery in Hubei province, China in December 2019. A combination of computed tomography imaging, whole genome sequencing, and electron microscopy were initially used to screen and identify SARS-CoV-2, the viral etiology of COVID-19. The aim of this review article is to inform the audience of diagnostic and surveillance technologies for SARS-CoV-2 and their performance characteristics. We describe point-of-care diagnostics that are on the horizon and encourage academics to advance their technologies beyond conception. Developing plug-and-play diagnostics to manage the SARS-CoV-2 outbreak would be useful in preventing future epidemics.
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              Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor

              Giwan Seo, Geonhee Lee, Mi Jeong Kim … (2020)
              Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.
              Article 0 comments Cited 737 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Talanta
                Journal ID (iso-abbrev): Talanta
                Title: Talanta
                Publisher: Elsevier B.V.
                ISSN (Print): 0039-9140
                ISSN (Electronic): 1873-3573
                Publication date PMC-release: 20 May 2021
                Publication date (Electronic): 20 May 2021
                Electronic Location Identifier: 122532
                Affiliations
                [a ]Department of Engineering, University of Campania “L. Vanvitelli”, Via Roma 29, 81031 Aversa, Italy
                [b ]Indivenire srl, Via Alla Cascata 56/C, 38123 Trento, Italy
                [c ]Fondazione Bruno Kessler-SD-MST, Via Sommarive 18, 38123 Trento, Italy
                [d ]CNR Institute of Biophysics, Via alla Cascata 56, Povo, 38123 Trento, Italy
                [e ]Fondazione Bruno Kessler-SD-MNF, Via Sommarive 18, 38123 Trento, Italy
                [f ]Department of Precision Medicine, University of Campania “L. Vanvitelli”, Vico L. De Crecchio 7, 80138 Napoli, Italy
                [g ]Biogem Institute of Molecular Biology and Genetics, Via Camporeale, 83031 Ariano Irpino, Italy
                Author notes
                [∗ ]Corresponding author.
                [∗∗ ]Corresponding author. ;
                [1]

                These authors have contributed equally to this work

                Article
                Publisher Item ID: S0039-9140(21)00453-7 Publisher ID: 122532
                DOI: 10.1016/j.talanta.2021.122532
                PMC ID: 8133803
                PubMed ID: 34215035
                SO-VID: a8a66d8a-6a62-4ec0-8446-78f13e226cf1
                Copyright © © 2021 Elsevier B.V. All rights reserved.
                License:

                Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

                History
                Date received : 7 March 2021
                Date revision received : 11 May 2021
                Date accepted : 13 May 2021
                Categories
                Subject: Article

                ScienceOpen disciplines: Analytical chemistry
                Keywords: self assembled monolayer (sam),aptamer,sars-cov-2,plastic optical fiber,polyethyleneglycol (peg),surface plasmon resonance (spr)
                Data availability:
                ScienceOpen disciplines: Analytical chemistry
                Keywords: self assembled monolayer (sam), aptamer, sars-cov-2, plastic optical fiber, polyethyleneglycol (peg), surface plasmon resonance (spr)

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