Maria V. Sinegubova: Role: Formal analysisRole: InvestigationRole: MethodologyRole: Writing – original
draft
Nadezhda A. Orlova:
ORCID: https://orcid.org/0000-0001-9483-2892
Role: ConceptualizationRole: Data curationRole: Formal analysisRole: InvestigationRole:
MethodologyRole: Project administrationRole: VisualizationRole: Writing – original
draftRole: Writing – review & editing
Sergey V. Kovnir: Role: InvestigationRole: Methodology
Lutsia K. Dayanova: Role: Investigation
Ivan I. Vorobiev:
ORCID: https://orcid.org/0000-0003-0064-4458
Role: ConceptualizationRole: Formal analysisRole: InvestigationRole: MethodologyRole:
SupervisionRole: Writing – original draftRole: Writing – review & editing
Paulo Lee Ho: Role: Editor
Journal ID (nlm-ta): PLoS One
Journal ID (iso-abbrev): PLoS One
Journal ID (publisher-id): plos
Journal ID (pmc): plosone
Title:
PLoS ONE
Publisher:
Public Library of Science
(San Francisco, CA USA
)
ISSN
(Electronic):
1932-6203
Publication date
(Electronic):
2
February
2021
Publication date Collection: 2021
Publication date PMC-release: 2
February
2021
Volume: 16
Issue: 2
Electronic Location Identifier: e0242890
Affiliations
[1
]
Laboratory of Mammalian Cell Bioengineering, Institute of Bioengineering, Research
Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia
[2
]
Laboratory of Glycoproteins Biotechnology, Institute of Bioorganic Chemistry of the
Russian Academy of Sciences, Moscow, Russia
Instituto Butantan, BRAZIL
Author notes
Competing Interests: MVS and LKD declare that they have no competing interests. SVK, NAO and IIV are inventors
of the patent RU2488633, which covers the use of the p1.1 plasmid; a derivative of
this plasmid is used in current research. The existence of the patent does not alter
our adherence to PLOS ONE policies on sharing data and materials.
Author information
Article
Publisher ID:
PONE-D-20-36259
DOI: 10.1371/journal.pone.0242890
PMC ID: 7853477
PubMed ID: 33529230
SO-VID: a1d9145f-cb11-43ca-8644-72df1984cff2
Copyright © © 2021 Sinegubova et al
License:
This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
The authors received no specific funding for this work.
Subject:
Research Article
Subject:
Biology and life sciences
Subject:
Organisms
Subject:
Viruses
Subject:
RNA viruses
Subject:
Coronaviruses
Subject:
SARS coronavirus
Subject:
SARS CoV 2
Subject:
Biology and life sciences
Subject:
Microbiology
Subject:
Medical microbiology
Subject:
Microbial pathogens
Subject:
Viral pathogens
Subject:
Coronaviruses
Subject:
SARS coronavirus
Subject:
SARS CoV 2
Subject:
Medicine and health sciences
Subject:
Pathology and laboratory medicine
Subject:
Pathogens
Subject:
Microbial pathogens
Subject:
Viral pathogens
Subject:
Coronaviruses
Subject:
SARS coronavirus
Subject:
SARS CoV 2
Subject:
Biology and life sciences
Subject:
Organisms
Subject:
Viruses
Subject:
Viral pathogens
Subject:
Coronaviruses
Subject:
SARS coronavirus
Subject:
SARS CoV 2
Subject:
Biology and Life Sciences
Subject:
Molecular Biology
Subject:
Molecular Biology Techniques
Subject:
Transfection
Subject:
Research and Analysis Methods
Subject:
Molecular Biology Techniques
Subject:
Transfection
Subject:
Medicine and Health Sciences
Subject:
Diagnostic Medicine
Subject:
Virus Testing
Subject:
Biology and life sciences
Subject:
Molecular biology
Subject:
Molecular biology techniques
Subject:
DNA construction
Subject:
Plasmid Construction
Subject:
Research and analysis methods
Subject:
Molecular biology techniques
Subject:
DNA construction
Subject:
Plasmid Construction
Subject:
Research and analysis methods
Subject:
Biological cultures
Subject:
Cell lines
Subject:
CHO cells
Subject:
Biology and Life Sciences
Subject:
Biochemistry
Subject:
Proteins
Subject:
Post-Translational Modification
Subject:
Signal Peptides
Subject:
Research and Analysis Methods
Subject:
Immunologic Techniques
Subject:
Immunoassays
Subject:
Enzyme-Linked Immunoassays
Subject:
Medicine and Health Sciences
Subject:
Pathology and Laboratory Medicine
Subject:
Serology