Simple and field amenable loop-mediated isothermal amplification-lateral flow dipstick assay for detection of west Nile virus in human clinical samples.

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Abstract

West Nile encephalitis caused by infection with the West Nile virus (WNV) is endemic in many regions of the world and is a global public health threat. The aim of this report was to develop a method using colorimetry-based reverse-transcription loop-mediated isothermal amplification (cRT-LAMP) and RT-LAMP combined with lateral-flow dipstick (LFD) for rapidly detecting WNV in low-infrastructure settings.

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Author and article information

Journal

Journal ID (iso-abbrev): J Appl Microbiol

Title: Journal of applied microbiology

Publisher: Wiley

ISSN (Electronic): 1365-2672

ISSN (Print): 1364-5072

Publication date (Electronic): Dec 2022

Volume: 133

Issue: 6

Affiliations

[1 ] Division of Virology, Defence Research and Development Establishment, Gwalior, India.

[2 ] School of Studies in Botany, Jiwaji University, Gwalior, India.

Article

DOI: 10.1111/jam.15783

PubMed ID: 36007965

SO-VID: a1b2bf6e-ec3b-4ee3-b11b-98ca6d09a929

History

Keywords: detection,WNV,LFD,LAMP,Env

Data availability:

Keywords: detection, WNV, LFD, LAMP, Env

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