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      mga genosensor for early detection of human rheumatic heart disease.

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          Abstract

          The 5' amino-labeled DNA probe complementary to mga gene of Streptococcus pyogenes was immobilized on carboxylated multiwall carbon nanotubes electrode and hybridized with 0.1-100 ng/6 μl single-stranded genomic DNA (ssG-DNA) of S. pyogenes from throat swab of suspected rheumatic heart disease (RHD) patients. Electrochemical response was measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance (EI). The sensitivity of the sensor was 106.03 (μA/cm(2))/ng and limit of detection (LOD) was found 0.014 ng/6 μl with regression coefficient (R(2)) of 0.921 using DPV. The genosensor was characterized by FTIR and SEM, and electrode was found stable for 6 months on storage at 4 °C with 5-6 % loss in initial DPV current. mga genosensor is the first report on RHD sensor which can save life of several suspected patients by early diagnosis in 30 min.

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          Author and article information

          Journal
          Appl Biochem Biotechnol
          Applied biochemistry and biotechnology
          Springer Science and Business Media LLC
          1559-0291
          0273-2289
          May 2014
          : 173
          : 1
          Affiliations
          [1 ] CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India.
          Article
          10.1007/s12010-014-0836-z
          24639090
          a0df4950-86de-4f9b-9fd2-2c251a1a77c9
          History

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