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      Caracterización molecular y ampelográfica de accesiones de vides pisqueras conservadas en un centro de colección de germoplasma de Ica, Perú Translated title: Molecular and ampelographic characterization of Pisco grapevine accessions conserved in a germplasm collection center of the Ica, Peru

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          Abstract

          Resumen El Pisco es un destilado de importancia cultural y económica para el Perú, elaborado con variedades de vid heterogéneas denominadas “pisqueras”, abarcadas en una legislación de Denominación de Origen Pisco. El objetivo fue confirmar la identidad varietal de ocho accesiones conservadas en la Colección de Germoplasma del Centro de Innovación Productiva y Transferencia Tecnológica Agroindustrial Ica (CITEagroindustrial), usando 9 loci microsatélites (SSR) estandarizados y 14 descriptores morfológicos según la Organización Internacional de la vid y el vino (OIV) para identificar variedades de vid. Los perfiles moleculares SSR de 7 accesiones se identificaron como variedades registradas en la Vitis International Variety Catalogue (VIVC) y solo una no tuvo asociación. De ellas, las accesiones correspondientes a Mollar, Albilla, Italia, Quebranta y Uvina se asociaron a las variedades Mollar Cano (VIVC 7901), Palomino Fino (VIVC 8888), Moscatel de Alejandría (VIVC 8241), Quebranta (VIVC 9840) y Jacquez (VIVC 5627), respectivamente. El perfil SSR de la accesión Torontel no se asoció a ninguna variedad registrada en la VIVC y las accesiones Negra Criolla y Moscatel se asociaron a la variedad española Listan Prieto (VIVC 6860). De acuerdo a las observaciones ampelográficas se determinó que Moscatel presentó variaciones fenotípicas respecto a Negra Criolla pese que a nivel molecular se asoció perfectamente a Listan Prieto, indicando que sería una variación clonal. Además, 7 variables fueron discriminantes para identificar las accesiones evaluadas. El análisis molecular y ampelográfico permitió esclarecer la identidad varietal de las accesiones conservadas en el CITEagroindustrial, Ica, la cual juega un rol preponderante como referente en cuanto a la identificación de las variedades empleadas para la elaboración de Pisco en Perú.

          Translated abstract

          Abstract Pisco is a distillate of cultural and economic importance for Peru, made from heterogeneous vine varieties called "pisqueras", covered by a Pisco Denomination of Origin legislation. The aim was to confirm the varietal identity of eight accessions conserved in the Germplasm Collection of the Centre for Productive Innovation and Technology Transfer Agroindustrial Ica (CITEagroindustrial), using 9 standardized microsatellite loci (SSR) and 14 morphological descriptors according to the International Organisation of Vine and Wine (OIV) to identify grapevine varieties. The SSR molecular profiles of 7 accessions were identified as varieties registered in the Vitis International Variety Catalog (VIVC) and only one had no association. Of these, Mollar, Albilla, Italia, Quebranta and Uvina accessions, were associated with Mollar Cano (VIVC 7901), Palomino Fino (VIVC 8888), Muscat of Alexandria (VIVC 8241), Quebranta (VIVC 9840) and Jacquez (VIVC 5627), respectively. The SSR profile of the Torontel accession was not associated with any variety registered in the VIVC and the Negra Criolla and Moscatel accessions were associated with the Spanish variety Listan Prieto (VIVC 6860). According to the ampelographic observations, it was determined that Moscatel presented phenotypic variations with respect to Negra Criolla, although at the molecular level it was perfectly associated with Listan Prieto, indicating that it would be a clonal variation. In addition, 7 variables were discriminant to identify the accessions evaluated. The molecular and ampelographic analysis allowed to clarify the varietal identity of the accessions conserved at CITEagroindustrial, Ica, which plays a preponderant role as a reference regarding the identification of the varieties used for the elaboration of Pisco in Peru.

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          A simple and efficient method for DNA extraction from grapevine cultivars andVitis species

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            Historical origins and genetic diversity of wine grapes.

            The genomic resources that are available to the grapevine research community have increased enormously during the past five years, in parallel with a renewed interest in grapevine (Vitis vinifera L.) germplasm resources and analysis of genetic diversity in grapes. Genetic variation, either natural or induced, is invaluable for crop improvement and understanding gene function, and the same is true for the grapevine. The history and vineyard cultural practices have largely determined the genetic diversity that exists today in grapevines. In this article, we provide a synopsis of what is known about the origin and genetics of grapes and how molecular genetics is helping us understand more about this plant: its evolution, historical development, genetic diversity and potential for genetic improvement.
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              A Rapid Total DNA Preparation Procedure for Fresh Plant Tissue

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                Author and article information

                Journal
                agro
                Scientia Agropecuaria
                Scientia Agropecuaria
                Universidad Nacional de Trujillo. Facultad de Ciencias Agropecuarias (Trujillo, , Peru )
                2077-9917
                October 2021
                : 12
                : 4
                : 525-533
                Affiliations
                [1] Lima Lima orgnameUniversidad Nacional Agraria La Molina orgdiv1Instituto de Biotecnología orgdiv2Laboratorio de Genómica y Bioinformática Peru
                [2] orgnameCentro de Innovación Productiva y Transferencia Tecnológica Agroindustrial orgdiv1CITEagroindustrial Ica Peru
                [3] Buenos Aires orgnameInstituto Nacional de Tecnología Agropecuaria orgdiv1Estación Experimental Agropecuaria Mendoza Argentina
                Article
                S2077-99172021000400525 S2077-9917(21)01200400525
                10.17268/sci.agropecu.2021.057
                98127e4d-fbe5-4865-97f1-6c5dd81bcab9

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 13 July 2021
                : 25 November 2021
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 44, Pages: 9
                Product

                SciELO Peru

                Categories
                Artículos de Investigación

                Pisco,Pisco varieties,criollas,SSR,Moscatel,Pisco.,variedades pisqueras

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