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Profiling cancer gene mutations in clinical formalin-fixed, paraffin-embedded colorectal tumor specimens using targeted next-generation sequencing.
Liangxuan Zhang
1 ,
Liangjing Chen ,
Sachin Sah ,
Gary J Latham ,
Rajesh Patel ,
Qinghua Song ,
Hartmut Koeppen ,
Rachel Tam ,
Erica Schleifman ,
Haider Mashhedi ,
Sreedevi Chalasani ,
Ling Fu ,
Teiko Sumiyoshi ,
Rajiv Raja ,
William Forrest ,
Garret M Hampton ,
Mark R Lackner ,
Priti Hegde ,
Shidong Jia
Apr 2014
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Abstract
The success of precision oncology relies on accurate and sensitive molecular profiling. The Ion AmpliSeq Cancer Panel, a targeted enrichment method for next-generation sequencing (NGS) using the Ion Torrent platform, provides a fast, easy, and cost-effective sequencing workflow for detecting genomic "hotspot" regions that are frequently mutated in human cancer genes. Most recently, the U.K. has launched the AmpliSeq sequencing test in its National Health Service. This study aimed to evaluate the clinical application of the AmpliSeq methodology.