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      Tensile Strength and Elastic Modulus of Gutta-percha Cones Disinfected with Sodium Hypochlorite at Different Immersion Times: An In Vitro Comparative Study

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          A BSTRACT

          Aim:

          The tensile strength and modulus of elasticity of gutta-percha cones can be chemically altered due to disinfectant solutions. Therefore, the aim of the present study was to compare tensile strength and elastic modulus of gutta-percha cones subjected to sodium hypochlorite (NaOCl) disinfection at different times.

          Materials and Methods:

          This in vitro and longitudinal experimental study consisted of 45 gutta-percha cones, divided equally into three groups: Group 1 (disinfection with 2.5% NaOCl), Group 2 (disinfection with 5.25% NaOCl), and control group. All groups were subdivided according to immersion times for 1, 5, and 10 minutes. Tensile strength and elastic modulus were measured with a universal testing machine. For comparing more than two independent groups, parametric analysis of variance test with Sheffe’s post hoc was used and for multivariate analysis, and multivariate analysis of variance test based on Pillai’s Trace was used. In all statistical analysis, a significance level P ≤ 0.05 was considered.

          Results:

          When comparing the tensile strength of gutta-percha cones, no significant differences were observed after being immersed at 1, 5, and 10 minutes in NaOCl 2.5% ( P = 0.715) and 5.25% ( P = 0.585). Regarding the elastic modulus, a significant decrease ( P < 0.05) was observed in those that were immersed in NaOCl 2.5% and 5.25% for 1, 5, and 10 minutes. Furthermore, increased NaOCl concentration significantly reduced the elastic modulus ( P < 0.001). However, there were no significant differences in tensile strength ( P > 0.05) and elastic modulus ( P > 0.05), when evaluating the interaction between NaOCl concentration and time.

          Conclusion:

          Increasing NaOCl concentration significantly reduced the modulus of elasticity without affecting the tensile strength of gutta-percha cones, regardless of immersion time. Furthermore, the interaction of time and NaOCl concentration did not significantly affect the tensile strength and elastic modulus.

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          Most cited references35

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          CRIS Guidelines (Checklist for Reporting In-vitro Studies): A concept note on the need for standardized guidelines for improving quality and transparency in reporting in-vitro studies in experimental dental research

          In vitro studies form a pivotal role in dental research contribution to a substantial evidence base. The reporting standards of these studies are not uniform thus resulting in lacunae in evidence reported. The effort of this concept note is to propose a Checklist for Reporting in vitro Studies (CRIS guidelines) that would promote quality and transparency in reporting in vitro studies.
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            Mechanism of action of sodium hypochlorite

            The choice of an irrigating solution for use in infected root canals requires previous knowledge of the microorganisms responsible for the infectious process as well as the properties of different irrigating solutions. Complex internal anatomy, host defenses and microorganism virulence are important factors in the treatment of teeth with asymptomatic apical periodontitis. Irrigating solutions must have expressive antimicrobial action and tissue dissolution capacity. Sodium hypochlorite is the most used irrigating solution in endodontics, because its mechanism of action causes biosynthetic alterations in cellular metabolism and phospholipid destruction, formation of chloramines that interfere in cellular metabolism, oxidative action with irreversible enzymatic inactivation in bacteria, and lipid and fatty acid degradation. The aim of this work is to discuss the mechanism of action of sodium hypochlorite based on its antimicrobial and physico-chemical properties.
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              Simulation-Based Power Analysis for Factorial Analysis of Variance Designs

              Researchers often rely on analysis of variance (ANOVA) when they report results of experiments. To ensure that a study is adequately powered to yield informative results with an ANOVA, researchers can perform an a priori power analysis. However, power analysis for factorial ANOVA designs is often a challenge. Current software solutions do not allow power analyses for complex designs with several within-participants factors. Moreover, power analyses often need [Formula: see text] or Cohen’s f as input, but these effect sizes are not intuitive and do not generalize to different experimental designs. We have created the R package Superpower and online Shiny apps to enable researchers without extensive programming experience to perform simulation-based power analysis for ANOVA designs of up to three within- or between-participants factors. Predicted effects are entered by specifying means, standard deviations, and, for within-participants factors, the correlations. The simulation provides the statistical power for all ANOVA main effects, interactions, and individual comparisons. The software can plot power across a range of sample sizes, can control for multiple comparisons, and can compute power when the homogeneity or sphericity assumption is violated. This Tutorial demonstrates how to perform a priori power analysis to design informative studies for main effects, interactions, and individual comparisons and highlights important factors that determine the statistical power for factorial ANOVA designs.
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                Author and article information

                Journal
                J Int Soc Prev Community Dent
                J Int Soc Prev Community Dent
                JISPCD
                J Int Soc Prevent Communit Dent
                Journal of International Society of Preventive & Community Dentistry
                Wolters Kluwer - Medknow (India )
                2231-0762
                2250-1002
                Jan-Feb 2024
                04 January 2024
                : 14
                : 1
                : 69-77
                Affiliations
                [1 ]Faculty of Dentistry and Postgraduate School, Universidad Nacional Federico Villarreal, Grupo de Investigación Salud y Bienestar Global, Lima, Peru
                [2 ]School of Stomatology, Universidad Privada San Juan Bautista, Lima, Peru
                [3 ]Faculty of Stomatology, Universidad Inca Garcilaso de la Vega, Lima, Peru
                [4 ]School of Stomatology, Universidad Cientifica del Sur, Lima, Peru
                Author notes
                Address for correspondence: Dr. César Félix Cayo-Rojas, Av. Jose Antonio Lavalle Avenue s/n (Ex Hacienda Villa), Chorrillos 15066, Lima, Peru. E-mail: cesarcayorojas@ 123456gmail.com
                Article
                JISPCD-14-69
                10.4103/jispcd.jispcd_106_22
                10980304
                38559640
                92bcd421-c5e0-4e36-9a91-b35a6bb84fc8
                Copyright: © 2024 Journal of International Society of Preventive and Community Dentistry

                This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.

                History
                : 28 April 2022
                : 20 January 2023
                : 20 January 2023
                Categories
                Original Article

                disinfection,elastic modulus,gutta-percha,sodium hypochlorite,tensile strength

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