11
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Cefazolin loaded chitosan nanoparticles to cure multi drug resistant Gram-negative pathogens.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Antibiotic resistance against Gram-negative microbes is considered as an alarming phenomenon that needs to be addressed urgently to develop better therapeutic solutions. The aim of the present research work was to investigate and develop cefazolin loaded chitosan nanoparticles (CSNPs) as a potential tool against multidrug resistant pathogens. Empty and drug loaded CSNPs were prepared by ionic gelation method. It was observed by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) based studies that CSNPs were less than 100 nm in size and displayed homogeneity both in shape and size. Encapsulation of cefazolin has not increased the size of nano systems. Zeta sizer results revealed that both systems have positive zeta potential of more or less +50 mV, thus contributing towards a stable formulation. Encapsulation efficiency was directly proportional to the increase in the concentration of antibiotic (28-62%). Furthermore, growth kinetics study had demonstrated excellent antimicrobial potential of cefazolin loaded CSNPs against multi drug resistant Klebsiella pneumoniae, Pseudomonas aeroginosa and Extended Spectrum Beta Lactamase (ESBL) positive Escherichia coli.

          Related collections

          Author and article information

          Journal
          Carbohydr Polym
          Carbohydrate polymers
          1879-1344
          0144-8617
          Jan 20 2016
          : 136
          Affiliations
          [1 ] Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Islamabad, Pakistan. Electronic address: bushrajamil2000@gmail.com.
          [2 ] Center for Micro and Nano Devices (CMND), COMSATS Institute of Information Technology, Islamabad, Pakistan.
          [3 ] Al-Sayed Hospital (Pvt) Ltd, 1-Hill Park, Opp. Ayub Park, Jhelum Road, Rawalpindi, Pakistan.
          [4 ] School of Chemical and Materials Engineering (SCME), National University of Science and Technology (NUST), H12, Islamabad, Pakistan.
          [5 ] Atta-ur-Rehman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H12, Islamabad, Pakistan.
          [6 ] Industrial Biotechnology Division, National Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
          [7 ] Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Islamabad, Pakistan.
          [8 ] Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Islamabad, Pakistan. Electronic address: m.imran@comsats.edu.pk.
          Article
          S0144-8617(15)00943-1
          10.1016/j.carbpol.2015.09.078
          26572401
          921842cd-95fe-4d75-bb86-e8cb37585246
          Copyright © 2015 Elsevier Ltd. All rights reserved.
          History

          Antibiotic resistance,Atomic force microscopy,Chitosan nanoparticles,ESBL,FTIR,Zeta sizer

          Comments

          Comment on this article