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      Determination of protein–protein interactions at the single-molecule level using optical tweezers

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          Abstract

          Biomolecular interactions are at the base of all physical processes within living organisms; the study of these interactions has led to the development of a plethora of different methods. Among these, single-molecule ( in singulo) experiments have become relevant in recent years because these studies can give insight into mechanisms and interactions that are hidden for ensemble-based ( in multiplo) methods. The focus of this review is on optical tweezer (OT) experiments, which can be used to apply and measure mechanical forces in molecular systems. OTs are based on optical trapping, where a laser is used to exert a force on a dielectric bead; and optically trap the bead at a controllable position in all three dimensions. Different experimental approaches have been developed to study protein–protein interactions using OTs, such as: (1) refolding and unfolding in trans interaction where one protein is tethered between the beads and the other protein is in the solution; (2) constant force in cis interaction where each protein is bound to a bead, and the tension is suddenly increased. The interaction may break after some time, giving information about the lifetime of the binding at that tension. And (3) force ramp in cis interaction where each protein is attached to a bead and a ramp force is applied until the interaction breaks. With these experiments, parameters such as kinetic constants ( k off, k on), affinity values ( K D), energy to the transition state Δ G , distance to the transition state Δ x can be obtained. These parameters characterize the energy landscape of the interaction. Some parameters such as distance to the transition state can only be obtained from force spectroscopy experiments such as those described here.

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                Author and article information

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                Journal
                Quarterly Reviews of Biophysics
                Quart. Rev. Biophys.
                Cambridge University Press (CUP)
                0033-5835
                1469-8994
                2022
                August 10 2022
                2022
                : 55
                Article
                10.1017/S0033583522000075
                35946323
                8995256c-87be-4b77-92ce-35475648e847
                © 2022

                https://www.cambridge.org/core/terms

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