For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
55
views
10
references
 Top references
cited by
75
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    recommends
      2
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Novel Mycobacterium tuberculosis Complex Pathogen, M. mungi

      brief-report

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Seven outbreaks involving increasing numbers of banded mongoose troops and high death rates have been documented. We identified a Mycobacterium tuberculosis complex pathogen, M. mungi sp. nov., as the causative agent among banded mongooses that live near humans in Chobe District, Botswana. Host spectrum and transmission dynamics remain unknown.

          Related collections

          Most cited references10

          • Record: found
          • Abstract: found
          • Article: not found

          Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference.

          E Engelke, M Barnard, Eileen Hoal … (2006)
          Differentiation of members of the Mycobacterium tuberculosis complex by conventional mycobacteriological methods is time consuming, making surveillance of species-specific disease difficult. A two-step, multiplex polymerase chain reaction (PCR) method based on genomic regions of difference (RD1, RD1(mic), RD2(seal), RD4, RD9 and RD12) was developed for the differentiation of M. canettii, M. tuberculosis, M. africanum, M. microti, M. pinnipedii, M. caprae, M. bovis and M. bovis BCG. The size of the respective multiplex PCR amplification products corresponded to the presence of the different M. tuberculosis complex members. This method allows for rapid differentiation, making it suitable for routine laboratories and surveillance purposes.
          Article 0 comments Cited 111 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Comparison of methods based on different molecular epidemiological markers for typing of Mycobacterium tuberculosis complex strains: interlaboratory study of discriminatory power and reproducibility.

            K. Kremer, D. Van Soolingen, R. Frothingham … (1999)
            In this study, the currently known typing methods for Mycobacterium tuberculosis isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 M. tuberculosis complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typing methods and in seven PCR-based assays. In all methods, one or more repetitive DNA elements were targeted. The strain typing and the DNA fingerprint analysis were performed in the laboratory most experienced in the respective method. To examine intralaboratory reproducibility, blinded duplicate samples were included. The specificities of the various methods were tested by inclusion of 10 non-M. tuberculosis complex strains. All five RFLP typing methods were highly reproducible. The reliability of the PCR-based methods was highest for the mixed-linker PCR, followed by variable numbers of tandem repeat (VNTR) typing and spoligotyping. In contrast, the double repetitive element PCR (DRE-PCR), IS6110 inverse PCR, IS6110 ampliprinting, and arbitrarily primed PCR (APPCR) typing were found to be poorly reproducible. The 90 strains were best discriminated by IS6110 RFLP typing, yielding 84 different banding patterns, followed by mixed-linker PCR (81 patterns), APPCR (71 patterns), RFLP using the polymorphic GC-rich sequence as a probe (70 patterns), DRE-PCR (63 patterns), spoligotyping (61 patterns), and VNTR typing (56 patterns). We conclude that for epidemiological investigations, strain differentiation by IS6110 RFLP or mixed-linker PCR are the methods of choice. A strong association was found between the results of different genetic markers, indicating a clonal population structure of M. tuberculosis strains. Several separate genotype families within the M. tuberculosis complex could be recognized on the basis of the genetic markers used.
            Article 0 comments Cited 103 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Novel genetic polymorphisms that further delineate the phylogeny of the Mycobacterium tuberculosis complex.

              Petra Haas, Debby Cousins, Michel Fabre … (2006)
              In a previous report, we described a PCR protocol for the differentiation of the various species of the Mycobacterium tuberculosis complex (MTC) on the basis of genomic deletions (R. C. Huard, L. C. de Oliveira Lazzarini, W. R. Butler, D. van Soolingen, and J. L. Ho, J. Clin. Microbiol. 41:1637-1650, 2003). That report also provided a broad cross-comparison of several previously identified, phylogenetically relevant, long-sequence and single-nucleotide polymorphisms (LSPs and SNPs, respectively). In the present companion report, we expand upon the previous work (i) by continuing the evaluation of known MTC phylogenetic markers in a larger collection of tubercle bacilli (n = 125), (ii) by evaluating additional recently reported MTC species-specific and interspecific polymorphisms, and (iii) by describing the identification and distribution of a number of novel LSPs and SNPs. Notably, new genomic deletions were found in various Mycobacterium tuberculosis strains, new species-specific SNPs were identified for "Mycobacterium canettii," Mycobacterium microti, and Mycobacterium pinnipedii, and, for the first time, intraspecific single-nucleotide DNA differences were discovered for the dassie bacillus, the oryx bacillus, and the two Mycobacterium africanum subtype I variants. Surprisingly, coincident polymorphisms linked one M. africanum subtype I genotype with the dassie bacillus and M. microti with M. pinnipedii, thereby suggesting closer evolutionary ties within each pair of species than had been previously thought. Overall, the presented data add to the genetic definitions of several MTC organisms as well as fine-tune current models for the evolutionary history of the MTC.
              Article 0 comments Cited 54 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Emerg Infect Dis
                Journal ID (publisher-id): EID
                Title: Emerging Infectious Diseases
                Publisher: Centers for Disease Control and Prevention
                ISSN (Print): 1080-6040
                ISSN (Electronic): 1080-6059
                Publication date (Print): August 2010
                Volume: 16
                Issue: 8
                Pages: 1296-1299
                Affiliations
                [1]Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA (K.A. Alexander, P.N. Laver)
                [2]Centre for Conservation of African Resources
                [3]Animals, Communities and Land Use, Kasane, Botswana (K.A. Alexander, P.N. Laver)
                [4]ARC-Onderstepoort Veterinary Institute, Pretoria, South Africa (A.L. Michel)
                [5]University of Pretoria, Pretoria (A.L. Michel, M. Williams)
                [6]Stellenbosch University, Tygerberg, South Africa (P.D. van Helden, R.M. Warren, N.C. Gey van Pittius)
                Author notes
                Address for correspondence: Kathleen A. Alexander, Virginia Polytechnic Institute and State University, Fisheries and Wildlife Sciences, 152 Cheatham Hall (0321), Blacksburg, VA 24061, USA; email: kathyalx@ 123456vt.edu
                Article
                Publisher ID: 10-0314
                DOI: 10.3201/eid1608.100314
                PMC ID: 3298296
                PubMed ID: 20678329
                SO-VID: 81b21953-25b3-4138-b79d-f63058a8c1f3
                History
                Categories
                Subject: Dispatch
                Subject: Dispatch

                ScienceOpen disciplines: Infectious disease & Microbiology
                Keywords: tuberculosis and other mycobacteria,human–wildlife interface,wildlife,banded mongoose,mycobacterium tuberculosis complex,tuberculosis,mycobacterium mungi,dispatch,dassie bacillius
                Data availability:
                ScienceOpen disciplines: Infectious disease & Microbiology
                Keywords: tuberculosis and other mycobacteria, human–wildlife interface, wildlife, banded mongoose, mycobacterium tuberculosis complex, tuberculosis, mycobacterium mungi, dispatch, dassie bacillius

                Comments

                Comment on this article