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Abstract

Chromosomal gene aac(6')-Ii of Enterococcus faecium CIP 54-32, encoding a 6'-N-aminoglycoside acetyltransferase was characterized. The gene was identified as a coding sequence of 549 bp corresponding to a protein with a calculated mass of 20,666 Da. Analysis of the sequence of the deduced protein suggested that it was the second member of a subfamily of AAC(6')-I enzymes. Insertional inactivation of aac(6')-Ii led to aminoglycoside susceptibility of CIP 54-32, suggesting that this gene plays a role in resistance to AAC(6')-I substrates. The gene was detected by DNA hybridization in all 26 strains of E. faecium tested but not in 44 other enterococci of 13 species. These data suggest that the aac(6')-Ii gene is species specific and may be used to identify E. faecium.

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PubMed ID:: 8239603

PMC ID:: 188089

DOI:: 10.1128/AAC.37.9.1896

ScienceOpen disciplines: Chemistry

Keywords: Acetyltransferases,genetics,Aminoglycosides,Anti-Bacterial Agents,metabolism,Bacterial Proteins,analysis,Base Sequence,Blotting, Southern,Chromosomes, Bacterial,Cloning, Molecular,DNA Transposable Elements,DNA, Bacterial,chemistry,Electrophoresis, Polyacrylamide Gel,Enterococcus faecium,enzymology,Escherichia coli,Molecular Sequence Data,Nucleic Acid Hybridization,Plasmids

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ScienceOpen disciplines: Chemistry

Keywords: Acetyltransferases, genetics, Aminoglycosides, Anti-Bacterial Agents, metabolism, Bacterial Proteins, analysis, Base Sequence, Blotting, Southern, Chromosomes, Bacterial, Cloning, Molecular, DNA Transposable Elements, DNA, Bacterial, chemistry, Electrophoresis, Polyacrylamide Gel, Enterococcus faecium, enzymology, Escherichia coli, Molecular Sequence Data, Nucleic Acid Hybridization, Plasmids

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