Chemical characterization of Brickellia cavanillesii (Asteraceae) using gas chromatographic methods

Abstract

A methanol extract of lyophilized Brickellia cavanillesii was quantitatively analyzed using gas chromatographic (GC) techniques. The chromatographic methods employed were (i) GC-flame ionization detector (GC-FID), (ii) GC-mass spectrometry (GC-MS), and (iii) purge and trap GC-MS (P&T GC-MS). Thirteen compounds were identified with a quality match of 90% and above using GC-MS. The compounds were ( 1) Cyclohexene, 6-ethenyl-6-methyl-1-(1-methylethyl)-3-(1-methylethylidene)-, (S)-; ( 2) Bicylo (2.2.1) heptan-2-one, 1, 7, 7-trimethyl-(1S, 4S)-; ( 3) Phenol, 2-methoxy-4-(1-propenyl)-; ( 4) Benzene, 1-(1, 5-dimethyl-4-hexenyl)-4-methyl-; ( 5) Naphthalene, 1, 2, 3, 5, 6, 8a-hexahydro4, 7-dimethyl-1-1-(1-methylethyl)-, (1S-cis)-; ( 6) Phenol, 2-methoxy-; ( 7) Benzaldehyde, 3-hydroxy-4-methoxy-; ( 8) 11, 13-Eicosadienoic acid, methyl ester; ( 9) 2-Furancarboxaldehyde, 5-methyl-; ( 10) Maltol; ( 11) Phenol; ( 12) Hydroquinone; ( 13) 1H-Indene, 1-ethylideneoctahydro-7a-methyl-, (1E, 3a.alpha, 7a.beta.). Other compounds ( 14) 3-methyl butanal; ( 15) (D)-Limonene; ( 16) 1-methyl-4-(1-methyl ethyl) benzene; ( 17) Butanoic acid methyl ester; ( 18) 2-methyl propanal; ( 19) 2-butanone; ( 20) 2-pentanone; and ( 21) 2-methyl butane were also identified when P&T GC-MS was performed. Of the 21 compounds identified, 12 were validated using chemical standards. The identified compounds were found to be terpenes, derivatives of terpenes, esters, ketones, aldehydes, and phenol-derived aromatic compounds; these are the primary constituents of the essential oils of many plants and flowers.