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Abstract
Yarrowia lipolytica LGAM S(7)1 presented remarkable growth on industrial glycerol
used as sole carbon substrate. Nitrogen-limited flask cultures were accompanied by
restricted synthesis of reserve lipid, whilst amounts of citric acid were produced
extracellularly. On the contrary, high amounts of reserve lipid (up to 3.5 g/l, 43%
w/w of lipids in dry biomass) were produced in highly aerated continuous cultures.
Lipid production was favoured at low specific dilution rates whilst fat-free material
yield increased over the whole range of D (h(-1)). The maximum volumetric productivity
obtained was 0.12 g lipid/1 h. Storage lipid composition did not present remarkable
changes in the specific dilution rates tested. Oleate and linoleate were the dominant
cellular fatty acids.