Comprehensive subspecies identification of 175 nontuberculous mycobacteria species based on 7547 genomic profiles

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

ABSTRACT

The prevalence of nontuberculous mycobacteria (NTM) pulmonary diseases has been increasing worldwide. NTM consist of approximately 200 species and distinguishing between them at the subspecies level is critical to treatment. In this study, we sequenced 63 NTM genomes, 27 of which were newly determined, by hybrid assembly using sequencers from Illumina and Oxford Nanopore Technologies (ONT). This analysis expanded the available genomic data to 175 NTM species and redefined their subgenus classification. We also developed a novel multi-locus sequence typing (MLST) database based on 184 genes from 7547 assemblies and an identification software, mlstverse, which can also be used for detecting other bacteria given a suitable MLST database. This method showed the highest sensitivity and specificity amongst conventional methods and demonstrated the capacity for rapid detection of NTM, 10 min of sequencing of the ONT MinION being sufficient. Application of this methodology could improve disease epidemiology and increase the cure rates of NTM diseases.

Related collections

Most cited references 24

Record: found
Abstract: found
Article: not found

MLST revisited: the gene-by-gene approach to bacterial genomics.

Martin C J Maiden, Melissa J Jansen van Rensburg, James E Bray … (2013)

Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data.

0 comments Cited 317 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: found

Is Open Access

Mycobacterium abscessus Complex Infections in Humans

Meng-Rui Lee, Wang-Huei Sheng, Chien-Ching Hung … (2015)

New treatments, rapid and inexpensive identification methods, and measures to contain nosocomial transmission and outbreaks are urgently needed.

0 comments Cited 214 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: found

Is Open Access

Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

Radhey S. Gupta, Brian K. Lo, Jeen Son (2018)

The genus Mycobacterium contains 188 species including several major human pathogens as well as numerous other environmental species. We report here comprehensive phylogenomics and comparative genomic analyses on 150 genomes of Mycobacterium species to understand their interrelationships. Phylogenetic trees were constructed for the 150 species based on 1941 core proteins for the genus Mycobacterium, 136 core proteins for the phylum Actinobacteria and 8 other conserved proteins. Additionally, the overall genome similarity amongst the Mycobacterium species was determined based on average amino acid identity of the conserved protein families. The results from these analyses consistently support the existence of five distinct monophyletic groups within the genus Mycobacterium at the highest level, which are designated as the “Tuberculosis-Simiae,” “Terrae,” “Triviale,” “Fortuitum-Vaccae,” and “Abscessus-Chelonae” clades. Some of these clades have also been observed in earlier phylogenetic studies. Of these clades, the “Abscessus-Chelonae” clade forms the deepest branching lineage and does not form a monophyletic grouping with the “Fortuitum-Vaccae” clade of fast-growing species. In parallel, our comparative analyses of proteins from mycobacterial genomes have identified 172 molecular signatures in the form of conserved signature indels and conserved signature proteins, which are uniquely shared by either all Mycobacterium species or by members of the five identified clades. The identified molecular signatures (or synapomorphies) provide strong independent evidence for the monophyly of the genus Mycobacterium and the five described clades and they provide reliable means for the demarcation of these clades and for their diagnostics. Based on the results of our comprehensive phylogenomic analyses and numerous identified molecular signatures, which consistently and strongly support the division of known mycobacterial species into the five described clades, we propose here division of the genus Mycobacterium into an emended genus Mycobacterium encompassing the “Tuberculosis-Simiae” clade, which includes all of the major human pathogens, and four novel genera viz. Mycolicibacterium gen. nov., Mycolicibacter gen. nov., Mycolicibacillus gen. nov. and Mycobacteroides gen. nov. corresponding to the “Fortuitum-Vaccae,” “Terrae,” “Triviale,” and “Abscessus-Chelonae” clades, respectively. With the division of mycobacterial species into these five distinct groups, attention can now be focused on unique genetic and molecular characteristics that differentiate members of these groups.

0 comments Cited 183 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): Emerg Microbes Infect

Journal ID (iso-abbrev): Emerg Microbes Infect

Journal ID (publisher-id): TEMI

Journal ID (publisher-id): temi20

Title: Emerging Microbes & Infections

Publisher: Taylor & Francis

ISSN (Electronic): 2222-1751

Publication date Collection: 2019

Publication date (Electronic): 9 July 2019

Volume: 8

Issue: 1

Pages: 1043-1053

Affiliations

[a ]Department of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University , Suita, Japan

[b ]Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus , Nakagami-gun, Japan

[c ]Laboratory of Pathogen Detection and Identification, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University , Suita, Japan

[d ]Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital , Nakagami-gun, Japan

[e ]Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University , Suita, Japan

Author notes

[CONTACT ] Takeshi Kinjo t_kinjo@ 123456med.u-ryukyu.ac.jp Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus , Nishihara-cho, Nakagami-gun, Okinawa, Japan, 903-0215

Shota Nakamura nshota@ 123456gen-info.osaka-u.ac.jp Department of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University , Suita, Osaka, Japan, 565–0871

Supplemental data for this article can be accessed https://doi.org/10.1080/22221751.2019.1637702.

Article

Publisher ID: 1637702

DOI: 10.1080/22221751.2019.1637702

PMC ID: 6691804

PubMed ID: 31287781

SO-VID: 7491a8ea-ff22-4b5b-9964-6742673b16eb

License:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

History

Date received : 27 February 2019

Date revision received : 05 June 2019

Date accepted : 24 June 2019

Page count

Figures: 5, Tables: 1, Equations: 0, References: 48, Pages: 11

Funding

Funded by: Japan Agency for Medical Research and Development 10.13039/100009619

Award ID: JP18gm1010005

Funded by: Japan Society for the Promotion of Science 10.13039/501100001691

Award ID: 16K09933,18K08431

Funded by: Ministry of Internal Affairs and Communications 10.13039/501100009105

Award ID: 172107106

This work was supported by the Okinawa Prefectural Government; Japan Agency for Medical Research and Development: [Grant Number JP18gm1010005]; Japan Society for the Promotion of Science: [Grant Number 16K09933,18K08431]; Ministry of Internal Affairs and Communications: [Grant Number #172107106].

Comments

Comment on this article

scite_

Smart Citations

Citing PublicationsSupportingMentioningContrasting

View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

Comprehensive subspecies identification of 175 nontuberculous mycobacteria species based on 7547 genomic profiles

Read this article at

ABSTRACT

Related collections

Arabidopsis genomics

Most cited references 24

MLST revisited: the gene-by-gene approach to bacterial genomics.

Mycobacterium abscessus Complex Infections in Humans

Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

Author and article information

Journal

Affiliations

Author notes

Article

History

Page count

Funding

Categories

Comments

Comment on this article

Similar content 597

Cited by 50

Most referenced authors 2,149