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Development and use of a vaccinia virus neutralization assay based on flow cytometric detection of green fluorescent protein.

Author(s): Bernard Moss, Patricia Earl, Jeffrey Americo

Publication date: 2003-09-30

Keywords: Animals, Cell Count, Flow Cytometry, methods, Green Fluorescent Proteins, HeLa Cells, Humans, Luminescent Proteins, analysis, Neutralization Tests, Rabbits, Smallpox Vaccine, immunology, Vaccination, Vaccinia virus, genetics

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Abstract

A rapid and sensitive neutralization assay is required to evaluate alternative smallpox vaccines. Here we describe the development and use of a 96-well plate, semi-automated, flow cytometric assay that uses a recombinant vaccinia virus expressing enhanced green fluorescent protein and which would be applicable to other viruses.

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PubMed ID:: 12970455

PMC ID:: 228521

ScienceOpen disciplines: Chemistry

Keywords: Animals,Cell Count,Flow Cytometry,methods,Green Fluorescent Proteins,HeLa Cells,Humans,Luminescent Proteins,analysis,Neutralization Tests,Rabbits,Smallpox Vaccine,immunology,Vaccination,Vaccinia virus,genetics

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ScienceOpen disciplines: Chemistry

Development and use of a vaccinia virus neutralization assay based on flow cytometric detection of green fluorescent protein.

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