Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars ( Sus scrofa) in Italy

Abstract

Leptospirosis is a re-emerging and globally spread zoonosis caused by pathogenic genomospecies of Leptospira. Wild boar ( Sus scrofa) are an important Leptospira host and are increasing in population all over Europe. The aim of this investigation was to evaluate Leptospira spp. infection in the reproductive systems of wild boar hunted in two Italian regions: Tuscany and Sardinia. From 231 animals, reproductive system tissue samples (testicles, epididymides, uteri) as well as placentas and fetuses were collected. Bacteriological examination and Real-Time PCR were performed to detect pathogenic Leptospira ( lipL32 gene). Leptospires were isolated from the testicles and epididymides of one adult and two subadult wild boar. Four isolates from the two subadult males were identified as Leptospira interrogans serogroup Australis by MLST, whereas Leptospira kirschneri serogroup Grippotyphosa was identified from the adult testicles and epididymis. Using Real-Time PCR, 70 samples were positive: 22 testicles (23.16%) and 22 epididymides (23.16%), 10 uteri (7.35%), 3 placentas (6.66%), and 13 fetuses (28.88%). Amplification of the rrs2 gene identified L. interrogans and L. kirschneri species. The results from this investigation confirmed that wild boar represent a potential source of pathogenic Leptospira spp. Isolation of Leptospira serogroups Australis and Grippotyphosa from the male reproductive system and the positive Real-Time PCR results from both male and female samples could suggest venereal transmission, as already demonstrated in pigs. Furthermore, placentas and fetuses were positive for the lipL32 target, and this finding may be related to a possible vertical transmission of pathogenic Leptospira.

Author summary

Leptospirosis is an infectious zoonotic disease present worldwide and is caused by bacteria belonging to genus Leptospira. Several domestic and wild animals could serve as reservoir hosts of these bacteria and could transmit them to humans, directly or indirectly. Generally, in asymptomatic carriers the infection involves the urinary system, the kidneys in particular, and leptospires are eliminated via urine. In some animals, Leptospira could also colonize the reproductive system as consequence of a systemic infection or venereal transmission. In this work, the Authors describe, for the first time, the detection of Leptospira from male and female reproductive organs of hunted wild boar via culture-dependent and -independent methods. These findings could suggest a possible vertical transmission in wild boar, but also a possible venereal transmission cycle among wild swine, which could accidentally involve domestic pigs too. Manipulation of these organs by hunters during slaughtering could also expose them to Leptospira infection. Obtained data add information on Leptospira epidemiology and highlight the importance of wildlife monitoring programs.