Bioactive bile salt-capped silver nanoparticles activity against destructive plant pathogenic fungi through in vitro system

Abstract

Sodium deoxycholate (NaDC) capped silver nanoparticles are employed to counteract the fungus Colletotrichum gloeosporioides which causes anthracnose disease in a wide number of plants throughout the world.

Abstract

Colletotrichum gloeosporioides is the most destructive endophytic plant-pathogenic fungi causing anthracnose disease in a wide number of economically important plants throughout the world. Presently there are no existing methods for effective disease management over the outbreak of anthracnose disease and it thus leads to huge economic losses for farmers and plant tissue culture laboratories. In order to find a new and effective control over these endophytes, we synthesized and characterized bioactive bile salt sodium deoxycholate (NaDC) capped silver nanoparticles. These nanoparticles were employed to control the endophytic fungus through in vitro direct and indirect model systems with time dependant/light mediated incubation manner. In our findings we achieved fivefold synergistic effect of NaDC-capped AgNPs with their bioactive capping agent against Colletotrichum gloeosporioides upon analyzing different parameters. Moreover, it was evident that NaDC-capped AgNPs did not cause any phytotoxicity to treated plants as revealed by molecular marker studies.