High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

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Abstract

Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.

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Journal

Journal ID (iso-abbrev): Nat Protoc

Title: Nature protocols

Publisher: Springer Science and Business Media LLC

ISSN (Electronic): 1750-2799

ISSN (Print): 1750-2799

Publication date (Electronic): 2007

Volume: 2

Issue: 1

Affiliations

[1 ] Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.

Article

Publisher Item ID: nprot.2007.13

DOI: 10.1038/nprot.2007.13

PubMed ID: 17401334

SO-VID: 5772d80f-6636-467e-aa06-0f10154d9b2b

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