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      Dengue Seroprevalence in the French West Indies: A Prospective Study in Adult Blood Donors

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          Abstract

          Using an anti-dengue immunoglobulin G (IgG) indirect enzyme-linked immunosorbent assay, seroprevalence was determined among 783 adult blood donors in the French Caribbean islands of Guadeloupe and Martinique in 2011. Overall, 93.5% [91.5; 95.1] samples were positive for dengue antibodies, 90.7% (350 of 386) in Martinique and 96.2% (382 of 397) in Guadeloupe. Only 30% of these adults recalled having had dengue disease before. Serotype-specific neutralization assays applied to a subset of IgG-positive samples indicated that a majority (77 of 96; 80%) reacted to the four serotypes. These seroprevalence findings are the first reported for Guadeloupe and Martinique and are consistent with the dengue epidemiology in these territories.

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          A real-time RT-PCR method for the universal detection and identification of flaviviruses.

          G Moureau, S Temmam, Jennifer Gonzalez … (2006)
          Here we describe an optimized molecular protocol for the universal detection and identification of flaviviruses. It combines the convenient real-time polymerase chain reaction (PCR) format with a broad spectrum of flavivirus detection. This assay, based on the amplification of a 269-272 nt (depending on the flavivirus tested) region at the N terminal end of the NS5 gene, enabled the amplification of 51 flavivirus species and 3 tentative species. Sequencing of the amplicons produced by reverse transcriptase (RT)-PCR permitted the reliable taxonomic identification of flavivirus species by comparison with reference sequences available in databases, using either the BLASTN algorithm or a simple phylogenetic reconstruction. The limit of detection of the assay (2-20,500 copies/reaction depending on the virus tested) allowed the detection of different flaviviruses from a series of human sera or veterinary samples. Altogether, the characteristics of this technique make it a good candidate for the identification of previously identified flaviviruses in cell culture and the investigation of field samples, and also a promising tool for the discovery and identification of new species, including viruses distantly related to "classical" arthropod-borne flaviviruses.
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            Complete nucleotide sequence of dengue type 3 virus genome RNA.

            K Osatomi, H Sumiyoshi (1990)
            The complete nucleotide sequence of the genome of the dengue virus type 3 was determined. Sequence analyses of the genomic RNA and cloned cDNA revealed that the genomic RNA contains 10,696 nucleotides and encodes a single open reading frame of 10,170 nucleotides corresponding to 3390 amino acid residues. The N-terminal amino acid sequences of three structural proteins (C, M, and E proteins) and the preM protein were also determined from the purified virion. When the deduced amino acid sequence and N-terminal amino acid sequence determined from purified proteins were compared with those of other flaviviruses, the genome organization was found to be the same as that of other flaviviruses.
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              Enzyme-linked immunosorbent assay-format microneutralization test for dengue viruses.

              Manuela Beltran, Vance Vorndam (2002)
              A microneutralization test that measures anti-dengue antibodies was developed. Serum dilutions, neutralization reactions, and virus growth were performed in 96-well plates. After incubation, an enzyme-linked immunosorbent assay that used mouse anti-dengue antibodies and an enzyme-conjugated anti-mouse antibody was used to measure cell-associated viral antigens. The resulting optical density readings were processed and graphed automatically by a spreadsheet program. This procedure provided results that are essentially the same as those from the plaque-reduction neutralization test for serum samples from primary dengue virus infections, but results correlated poorly with results from samples from people with secondary infections. The test offers the advantages of ease of performance, ease in the calculation of results, lower cost, and increased speed.
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                Author and article information

                Journal
                Journal ID (nlm-ta): Am J Trop Med Hyg
                Journal ID (iso-abbrev): Am. J. Trop. Med. Hyg
                Journal ID (publisher-id): tpmd
                Title: The American Journal of Tropical Medicine and Hygiene
                Publisher: The American Society of Tropical Medicine and Hygiene
                ISSN (Print): 0002-9637
                ISSN (Electronic): 1476-1645
                Publication date (Print): 03 June 2015
                Publication date PMC-release: 03 June 2015
                Volume: 92
                Issue: 6
                Pages: 1137-1140
                Affiliations
                Global Epidemiology Department, Sanofi Pasteur, Lyon, France; EA 4537 Université des Antilles et de la Guyane; Centre d'Investigation clinique des Antilles et de la Guyane, CIC1424, Cayenne, Guyane; UMR_D 190 “Emergence des Pathologies Virales”, Marseille, France; Aix Marseille University, IRD French Institute of Research for Development, EHESP French School of Public Health; Service des Maladies Infectieuses et Tropicales, CHU de Martinique, Fort-de-France, Martinique; Laboratoire de Virologie, CHU de Martinique, Fort-de-France, Martinique; Etablissement Français du Sang, Pointe à Pitre, Guadeloupe; Etablissement Français du Sang, Hôpital Pierre Zobda Quitman, Fort-de-France, Martinique
                Author notes
                *Address correspondence to Maïna L'Azou, Sanofi Pasteur Global Epidemiology Department 2, Avenue pont Pasteur 69367 Lyon cedex 07, France. E-mail: maina.lazou@ 123456sanofipasteur.com
                Article
                DOI: 10.4269/ajtmh.14-0211
                PMC ID: 4458816
                PubMed ID: 25846291
                SO-VID: 52d22fe3-de62-4019-a035-7e07bdbfc38a
                Copyright © ©The American Society of Tropical Medicine and Hygiene
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                Date received : 08 April 2014
                Date accepted : 22 December 2014
                Categories
                Subject: Articles

                ScienceOpen disciplines: Infectious disease & Microbiology
                Data availability:
                ScienceOpen disciplines: Infectious disease & Microbiology

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