1
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Inducción de mecanismos de defensivos en plantas de tomate (Solanum lycopersicum L. cv. Amalia) con el uso de aceites esenciales Translated title: Induction of defence mechanisms in tomato (Solanum lycopersicum L. cv. Amalia) plants by essential oils

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Se estudió el efecto de diferentes aceites esenciales en la inducción de mecanismos defensivos en tomate (Solanum lycopersicum L. cv. Amalia). Las plantas se asperjaron con diferentes aceites e inductores como el Bion, y se tomaron muestras de hojas a las 24, 72 y 144 horas posteriores a la aspersión. Se determinó la concentración de proteínas totales, así como la actividad enzimática específica de peroxidasas, polifenoloxidasas, fenilalanina amonio liasas y quitinasas. Los aceites esenciales obtenidos a partir de subproductos de la industria de cítricos y de melaleuca (Melaleuca quinquenervia (Cav) S.T. Blake) indujeron las mayores actividades enzimáticas de peroxidasas, polifenoloxidasas y quitinasas. El ácido jasmónico y los fosfolípidos fueron los responsables de la inducción de fenilalanina amonio liasas.

          Translated abstract

          In this work, the effect of different essential oils was evaluated using tomato cv. Amalia plants. The plants were sprayed with different oils and inductors as Bion, and leave samples were taken at 24, 72 and 144 hours after spray. Total protein concentration and specific enzymatic activities of peroxidases, polyphenoloxidases, phenylalanine ammonia lyase and chitinases were determined. The essential oils obtained from citric fruit scraps and Melaleuca quinquenervia (Cav) S.T. Blake induced higher peroxidases, polyphenoloxidases and chitinases enzymatic activities. Jasmonic acid and phospholipids were responsible for phenylalanine ammonia lyase induction.

          Related collections

          Most cited references23

          • Record: found
          • Abstract: not found
          • Article: not found

          A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Plant Glandular Trichomes as Targets for Breeding or Engineering of Resistance to Herbivores

            Glandular trichomes are specialized hairs found on the surface of about 30% of all vascular plants and are responsible for a significant portion of a plant’s secondary chemistry. Glandular trichomes are an important source of essential oils, i.e., natural fragrances or products that can be used by the pharmaceutical industry, although many of these substances have evolved to provide the plant with protection against herbivores and pathogens. The storage compartment of glandular trichomes usually is located on the tip of the hair and is part of the glandular cell, or cells, which are metabolically active. Trichomes and their exudates can be harvested relatively easily, and this has permitted a detailed study of their metabolites, as well as the genes and proteins responsible for them. This knowledge now assists classical breeding programs, as well as targeted genetic engineering, aimed to optimize trichome density and physiology to facilitate customization of essential oil production or to tune biocide activity to enhance crop protection. We will provide an overview of the metabolic diversity found within plant glandular trichomes, with the emphasis on those of the Solanaceae, and of the tools available to manipulate their activities for enhancing the plant’s resistance to pests.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function.

              Ethylene induced an endochitinase in primary leaves of Phaseolus vulgaris L. The enzyme formed chitobiose and higher chitin oligosaccharides from insoluble, colloidal or regenerated chitin. Less than 5% of the total chitinolytic activity was detected in an exochitinase assay proposed by Abeles et al. (1970, Plant Physiol. 47, 129-134) for ethylene-induced chitinase. In ethylene-treated plants, chitinase activity started to increase after a lag of 6 h and was induced 30 fold within 24 h. Exogenously supplied ethylene at 1 nl ml(-1) was sufficient for half-maximal induction, and enhancement of the endogenous ethylene formation also enhanced chitinase activity. Cycloheximide prevented the induction. Among various hydrolases tested, only chitinase and, to a lesser extent, β-1,3-glucanase were induced by ethylene. Induction of chitinase by ethylene occurred in many different plant species. Ethylene-induced chitinase was purified by affinity chromatography on a column of regenerated chitin. Its apparent molecular weight obtained by sodium dodecyl sulfate-gel electrophoresis was 30,000; the molecular weight determined from filtration through Sephadex G-75 was 22,000. The purified enzyme attacked chitin in isolated cell walls of Fusarium solani. It also acted as a lysozyme when incubated with Micrococcus lysodeikticus. It is concluded that ethylene-induced chitinase functions as a defense enzyme against fungal and bacterial invaders.
                Bookmark

                Author and article information

                Journal
                rpv
                Revista de Protección Vegetal
                Rev. Protección Veg.
                Centro Nacional de Sanidad Agropecuaria (La Habana, , Cuba )
                1010-2752
                2224-4697
                December 2013
                : 28
                : 3
                : 185-191
                Affiliations
                [01] Mayabeque orgnameCentro Nacional de Sanidad Agropecuaria (CENSA) orgdiv1Dirección de Sanidad Vegetal Cuba marquetti@ 123456censa.edu.cu
                Article
                S1010-27522013000300004 S1010-2752(13)02800304
                4055ebc7-0685-4281-a7af-23e74185969e

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 01 November 2013
                : 30 August 2013
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 23, Pages: 7
                Product

                SciELO Cuba

                Self URI: Texto completo solamente en formato PDF (ES)
                Categories
                TRABAJOS ORIGINALES

                Bion,phospholipids,Fitomas,jasmonic acid,fosfolípidos,ácido jasmónico

                Comments

                Comment on this article