Electrospun cellulose acetate/gelatin nanofibrous wound dressing containing berberine for diabetic foot ulcer healing:  in vitro  and  in vivo  studies

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Abstract

Functional wound dressing with tailored physicochemical and biological properties is vital for diabetic foot ulcer (DFU) treatment. Our main objective in the current study was to fabricate Cellulose Acetate/Gelatin (CA/Gel) electrospun mat loaded with berberine (Beri) as the DFU-specific wound dressing. The wound healing efficacy of the fabricated dressings was evaluated in streptozotocin-induced diabetic rats. The results demonstrated an average nanofiber diameter of 502 ± 150 nm, and the tensile strength, contact angle, porosity, water vapor permeability and water uptake ratio of CA/Gel nanofibers were around 2.83 ± 0.08 MPa, 58.07 ± 2.35°, 78.17 ± 1.04%, 11.23 ± 1.05 mg/cm ²/hr, and 12.78 ± 0.32%, respectively, while these values for CA/Gel/Beri nanofibers were 2.69 ± 0.05 MPa, 56.93 ± 1°, 76.17 ± 0.76%, 10.17 ± 0.21 mg/cm ²/hr, and 14.37 ± 0.42%, respectively. The antibacterial evaluations demonstrated that the dressings exhibited potent antibacterial activity. The collagen density of 88.8 ± 6.7% and the angiogenesis score of 19.8 ± 3.8 obtained in the animal studies indicate a proper wound healing. These findings implied that the incorporation of berberine did not compromise the physical properties of dressing, while improving the biological activities. In conclusion, our results indicated that the prepared mat is a proper wound dressing for DFU management and treatment.

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Nanofiber technology: designing the next generation of tissue engineering scaffolds.

Catherine Barnes, Scott Sell, Eugene Boland … (2007)

Tissue engineering is an interdisciplinary field that has attempted to utilize a variety of processing methods with synthetic and natural polymers to fabricate scaffolds for the regeneration of tissues and organs. The study of structure-function relationships in both normal and pathological tissues has been coupled with the development of biologically active substitutes or engineered materials. The fibrillar collagens, types I, II, and III, are the most abundant natural polymers in the body and are found throughout the interstitial spaces where they function to impart overall structural integrity and strength to tissues. The collagen structures, referred to as extracellular matrix (ECM), provide the cells with the appropriate biological environment for embryologic development, organogenesis, cell growth, and wound repair. In the native tissues, the structural ECM proteins range in diameter from 50 to 500 nm. In order to create scaffolds or ECM analogues, which are truly biomimicking at this scale, one must employ nanotechnology. Recent advances in nanotechnology have led to a variety of approaches for the development of engineered ECM analogues. To date, three processing techniques (self-assembly, phase separation, and electrospinning) have evolved to allow the fabrication of nanofibrous scaffolds. With these advances, the long-awaited and much anticipated construction of a truly "biomimicking" or "ideal" tissue engineered environment, or scaffold, for a variety of tissues is now highly feasible. This review will discuss the three primary technologies (with a focus on electrospinning) available to create tissue engineering scaffolds that are capable of mimicking native tissue, as well as explore the wide array of materials investigated for use in scaffolds.

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Recent advances on the development of wound dressings for diabetic foot ulcer treatment--a review.

F. Dias, Joao Sousa, Liane I F Moura … (2013)

Diabetic foot ulcers (DFUs) are a chronic, non-healing complication of diabetes that lead to high hospital costs and, in extreme cases, to amputation. Diabetic neuropathy, peripheral vascular disease, abnormal cellular and cytokine/chemokine activity are among the main factors that hinder diabetic wound repair. DFUs represent a current and important challenge in the development of novel and efficient wound dressings. In general, an ideal wound dressing should provide a moist wound environment, offer protection from secondary infections, remove wound exudate and promote tissue regeneration. However, no existing dressing fulfills all the requirements associated with DFU treatment and the choice of the correct dressing depends on the wound type and stage, injury extension, patient condition and the tissues involved. Currently, there are different types of commercially available wound dressings that can be used for DFU treatment which differ on their application modes, materials, shape and on the methods employed for production. Dressing materials can include natural, modified and synthetic polymers, as well as their mixtures or combinations, processed in the form of films, foams, hydrocolloids and hydrogels. Moreover, wound dressings may be employed as medicated systems, through the delivery of healing enhancers and therapeutic substances (drugs, growth factors, peptides, stem cells and/or other bioactive substances). This work reviews the state of the art and the most recent advances in the development of wound dressings for DFU treatment. Special emphasis is given to systems employing new polymeric biomaterials, and to the latest and innovative therapeutic strategies and delivery approaches. Copyright © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

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Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications.

Hua Li, J MacDiarmid, Kuo-Yen Wei … (2006)

Polyaniline (PANi), a conductive polymer, was blended with a natural protein, gelatin, and co-electrospun into nanofibers to investigate the potential application of such a blend as conductive scaffold for tissue engineering purposes. Electrospun PANi-contained gelatin fibers were characterized using scanning electron microscopy (SEM), electrical conductivity measurement, mechanical tensile testing, and differential scanning calorimetry (DSC). SEM analysis of the blend fibers containing less than 3% PANi in total weight, revealed uniform fibers with no evidence for phase segregation, as also confirmed by DSC. Our data indicate that with increasing the amount of PANi (from 0 to approximately 5%w/w), the average fiber size was reduced from 803+/-121 nm to 61+/-13 nm (p<0.01) and the tensile modulus increased from 499+/-207 MPa to 1384+/-105 MPa (p<0.05). The results of the DSC study further strengthen our notion that the doping of gelatin with a few % PANi leads to an alteration of the physicochemical properties of gelatin. To test the usefulness of PANi-gelatin blends as a fibrous matrix for supporting cell growth, H9c2 rat cardiac myoblast cells were cultured on fiber-coated glass cover slips. Cell cultures were evaluated in terms of cell proliferation and morphology. Our results indicate that all PANi-gelatin blend fibers supported H9c2 cell attachment and proliferation to a similar degree as the control tissue culture-treated plastic (TCP) and smooth glass substrates. Depending on the concentrations of PANi, the cells initially displayed different morphologies on the fibrous substrates, but after 1 week all cultures reached confluence of similar densities and morphology. Taken together these results suggest that PANi-gelatin blend nanofibers might provide a novel conductive material well suited as biocompatible scaffolds for tissue engineering.

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Author and article information

Contributors

Majid Salehi: msalehi.te1392@gmail.com

Journal

Journal ID (nlm-ta): Sci Rep

Journal ID (iso-abbrev): Sci Rep

Title: Scientific Reports

Publisher: Nature Publishing Group UK (London )

ISSN (Electronic): 2045-2322

Publication date (Electronic): 20 May 2020

Publication date PMC-release: 20 May 2020

Publication date Collection: 2020

Volume: 10

Electronic Location Identifier: 8312

Affiliations

[1 ]ISNI 0000 0001 2012 5829, GRID grid.412112.5, Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, ; Kermanshah, Iran

[2 ]ISNI 0000 0004 1936 9430, GRID grid.21100.32, Department of Kinesiology and Health Science, , York University, ; Ontario, Canada

[3 ]GRID grid.472472.0, Department of Mechanical Engineering, , Science and Research Branch, Islamic Azad University, ; Tehran, Iran

[4 ]ISNI 0000 0001 0166 0922, GRID grid.411705.6, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, , Tehran University of Medical Sciences, ; Tehran, Iran

[5 ]ISNI 0000 0000 8819 4698, GRID grid.412571.4, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Medical Sciences and Technologies, , Shiraz University of Medical Sciences, ; Shiraz, Iran

[6 ]ISNI 0000 0004 0384 8816, GRID grid.444858.1, School of Medicine, , Shahroud University of Medical Sciences, ; Shahroud, Iran

[7 ]GRID grid.411600.2, Department of Oral and Maxillofacial Surgery, , Dental School, Shahid Beheshti University of Medical sciences, ; Tehran, Iran

[8 ]ISNI 0000 0001 0166 0922, GRID grid.411705.6, Dental student of scientific research center, faculty of dentistry, Tehran university of medical sciences, ; Tehran, Iran

[9 ]ISNI 0000 0001 2323 3518, GRID grid.262613.2, Department of Biomedical Engineering, Rochester Institute of Technology, ; Rochester, USA

[10 ]ISNI 0000 0001 2323 3518, GRID grid.262613.2, Department of Microsystems Engineering, , Rochester Institute of Technology, ; Rochester, NY USA

[11 ]ISNI 0000 0004 0415 3047, GRID grid.411135.3, Department of Tissue Engineering, , School of Advanced Technologies, Fasa University of Medical Sciences, ; Fasa, Iran

[12 ]ISNI 0000 0004 0384 8816, GRID grid.444858.1, Department of Tissue Engineering, School of Medicine, , Shahroud University of Medical Sciences, ; Shahroud, Iran

[13 ]ISNI 0000 0004 0384 8816, GRID grid.444858.1, Tissue Engineering and stem cells research center, Shahroud University of Medical Sciences, ; Shahroud, Iran

Article

Publisher ID: 65268

DOI: 10.1038/s41598-020-65268-7

PMC ID: 7239895

PubMed ID: 32433566

SO-VID: 3c3ca920-c3f4-4c92-9afc-798e282bd422

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

History

Date received : 3 March 2020

Date accepted : 27 April 2020

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ScienceOpen disciplines: Uncategorized

Keywords: nanomedicine,nanoscale materials,antimicrobials

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ScienceOpen disciplines: Uncategorized

Keywords: nanomedicine, nanoscale materials, antimicrobials

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Electrospun cellulose acetate/gelatin nanofibrous wound dressing containing berberine for diabetic foot ulcer healing: in vitro and in vivo studies

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Most cited references 50

Nanofiber technology: designing the next generation of tissue engineering scaffolds.

Recent advances on the development of wound dressings for diabetic foot ulcer treatment--a review.

Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications.

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