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      The detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons.

      The Journal of General Virology
      Amino Acid Sequence, Animals, Base Sequence, Cell Nucleus, virology, Herpesvirus 1, Equid, genetics, physiology, Herpesvirus 1, Human, Horses, In Situ Hybridization, Molecular Sequence Data, Promoter Regions, Genetic, RNA Probes, RNA, Antisense, analysis, RNA, Messenger, RNA, Viral, Specific Pathogen-Free Organisms, Trigeminal Ganglion, Viral Proteins, Virus Latency, Virus Shedding

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          Abstract

          Neural tissues from specific pathogen-free ponies that had been experimentally infected with equine herpesvirus 1 (EHV-1) were analysed by in situ hybridization. Digoxigenin-labelled EHV-1 BamHI fragments spanning almost the entire EHV-1 genome were hybridized to RNA in tissue sections from latently infected trigeminal ganglia. The BamHI E fragment detected EHV-1 RNA antisense to gene 63 (HSV-1 homologue ICP0) in a small number of neurons. Sixteen other BamHI fragments gave negative results in 20 sections tested with each fragment. Latency associated transcripts (LATs) were localized to the neuronal nuclei. EHV-1 nucleotide sequence data in the region reveals the presence of a putative EHV-1 LAT promoter that shares a similar motifs with the HSV-1 LAT promoter, including the LAT promoter-binding factor, and may have a role in EHV-1 LAT expression.

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