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      Elasticity and electrostatics of plectonemic DNA.

      1 , ,
      Biophysical journal
      Elsevier BV

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          Abstract

          We present a self-contained theory for the mechanical response of DNA in single molecule experiments. Our model is based on a one-dimensional continuum description of the DNA molecule and accounts both for its elasticity and for DNA-DNA electrostatic interactions. We consider the classical loading geometry used in experiments where one end of the molecule is attached to a substrate and the other one is pulled by a tensile force and twisted by a given number of turns. We focus on configurations relevant to the limit of a large number of turns, which are made up of two phases, one with linear DNA and the other one with superhelical DNA. The model takes into account thermal fluctuations in the linear phase and electrostatic interactions in the superhelical phase. The values of the torsional stress, of the supercoiling radius and angle, and key features of the experimental extension-rotation curves, namely the slope of the linear region and thermal buckling threshold, are predicted. They are found in good agreement with experimental data.

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          Author and article information

          Journal
          Biophys. J.
          Biophysical journal
          Elsevier BV
          1542-0086
          0006-3495
          May 06 2009
          : 96
          : 9
          Affiliations
          [1 ] Université Pierre et Marie Curie, University Paris, UMR 7190, Institut Jean Le Rond d'Alembert, Paris, France.
          Article
          S0006-3495(09)00564-5
          10.1016/j.bpj.2009.02.032
          2711414
          19413977
          36306ef2-72af-4e99-baf1-026a727dd5dd
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