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      High burden of ESBL- producing Klebsiella spp., Proteus mirabilis, Enterobacter cloacae and Pseudomonas aeruginosa in diagnosed cases of urinary tract infection in a Nigerian Teaching Hospital.

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          Abstract

          Infections of the urinary tract have been on the rise globally and these are also worsened by the increasing rate of antibiotic resistance in uropathogens. This study aimed to determine the susceptibility profile of extended spectrum β-lactamase (ESBL)- producing uropathogens to selected antibiotics and their carriage of ESBL genes. Bacterial uropathogens were obtained from the urine bench of a Microbiology laboratory in a Teaching Hospital in South-West Nigeria. Susceptibility to antibiotics was tested using the disc diffusion method, while detection of ESBL production was done using the double disc synergy test (DDST). Detection of ESBL genes was performed by PCR. A total of 21 ESBL- producing uropathogens were obtained namely: Klebsiella pneumoniae (11), Klebsiella oxytoca (6), Proteus mirabilis (2), Enterobacter cloacae (1) and Pseudomonas aeruginosa (1). The resistance to antibiotics in the uropathogens was: imipenem (0%), gentamicin (38.1%), sulfamethoxazole-trimethoprim (52.4%), amoxicillin-clavulanate (61.9%), aztreonam (66.7%), ceftazidime (66.7%), tetracycline (90.5%), cefpodoxime (100%) and cefotaxime (100%). Altogether, 90.5% (19/21) of the isolates were multidrug resistant (MDR). Of the 21 uropathogens, 61.9% (13/21) carried bla CTX-M, 52.4% (11/21) carried bla TEM while bla SHV was detected in 47.6% (10/21) of the isolates. There was co-carriage of ESBL genes in 12 uropathogens. This study showed a high prevalence of multidrug resistance and a high carriage of ESBL genes in the ESBL- producing isolates obtained over the study period. There is a need for a review of antibiotic options in the treatment of UTI to clamp down on the ever-increasing tide of antibiotic resistance in uropathogens.

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          Author and article information

          Journal
          Acta Microbiol Immunol Hung
          Acta microbiologica et immunologica Hungarica
          Akademiai Kiado Zrt.
          1588-2640
          1217-8950
          Jun 16 2022
          : 69
          : 2
          Affiliations
          [1 ] 1 Environmental Microbiology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Ibadan, Nigeria.
          [2 ] 2 Molecular Biology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Ibadan, Nigeria.
          [3 ] 3 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria.
          [4 ] 4 Pathogenic Microbiology Laboratory, Department of Microbiology, University of Ibadan, Ibadan, Nigeria.
          Article
          10.1556/030.2022.01747
          35895567
          291d3c64-baea-4fc5-902a-ba3c506847bb
          History

          urinary tract infection (UTI),Polymerase Chain Reaction (PCR),Teaching Hospital,bacterial uropathogens,blaCTX-M,blaSHV,blaTEM,extended spectrum β-lactamase (ESBL),multidrug resistance

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