Intestinal microbiota changes pre- and post-fecal microbiota transplantation for treatment of recurrent <i>Clostridioides difficile</i> infection among Iranian patients with concurrent inflammatory bowel disease

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Abstract

Introduction

Patients with inflammatory bowel disease (IBD) are at a greater risk for the recurrence of Clostridioides difficile infection (rCDI) that is triggered by intestinal microbiota dysbiosis. Fecal microbiota transplantation (FMT) has emerged as a highly effective therapeutic option for this complication. However, little is known about the impact of FMT on intestinal microbiota alterations in rCDI patients suffering from IBD. In this study, we aimed to investigate post-FMT intestinal microbiota alterations in Iranian rCDI patients with underlying IBD.

Methods

A total of 21 fecal samples were collected including 14 samples pre- and post-FMT and 7 samples from healthy donors. Microbial analysis was performed by quantitative real-time PCR (RT-qPCR) assay targeting the 16S rRNA gene. The pre-FMT profile and composition of the fecal microbiota were compared to the microbial changes of samples collected 28 days after FMT.

Results and discussion

Overall, the fecal microbiota profile of recipients was more similar to donor samples after the transplantation. We observed a significant increase in the relative abundance of Bacteroidetes post-FMT, compared to the pre-FMT microbial profile. Furthermore, there were remarkable differences between the microbial profile of pre-FMT, post-FMT, and healthy donor samples by PCoA analysis based on the ordination distance. This study demonstrates FMT as a safe and effective approach to restore the indigenous composition of the intestinal microbiota in rCDI patients and ultimately results in the treatment of concurrent IBD.

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The human microbiome project.

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A strategy to understand the microbial components of the human genetic and metabolic landscape and how they contribute to normal physiology and predisposition to disease.

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Author and article information

Contributors

Masoumeh Azimirad: URI : http://loop.frontiersin.org/people/566043/overview

Kaveh Naseri: URI : http://loop.frontiersin.org/people/1134738/overview

Ali Nabavi-Rad: URI : http://loop.frontiersin.org/people/1599363/overview

Hamid Asadzadeh Aghdaei: URI : http://loop.frontiersin.org/people/1111571/overview

Shabnam Shahrokh: URI : http://loop.frontiersin.org/people/1627634/overview

Abbas Yadegar: URI : http://loop.frontiersin.org/people/713435/overview

Journal

Journal ID (nlm-ta): Front Microbiol

Journal ID (iso-abbrev): Front Microbiol

Journal ID (publisher-id): Front. Microbiol.

Title: Frontiers in Microbiology

Publisher: Frontiers Media S.A.

ISSN (Electronic): 1664-302X

Publication date (Electronic): 24 February 2023

Publication date Collection: 2023

Volume: 14

Electronic Location Identifier: 1147945

Affiliations

[1] ¹Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences , Tehran, Iran

[2] ²Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences , Tehran, Iran

[3] ³Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences , Tehran, Iran

[4] ⁴Department of Gastroenterology and Hepatology, Tehran University of Medical Sciences , Tehran, Iran

Author notes

Edited by: George Grant, University of Aberdeen, United Kingdom

Reviewed by: Marcos Edgar Herkenhoff, University of São Paulo, Brazil; Felix Broecker, Idorsia Pharmaceuticals Ltd., Switzerland

*Correspondence: Abbas Yadegar, a.yadegar@ 123456sbmu.ac.ir , babak_y1983@ 123456yahoo.com

^†ORCID: Ali Nabavi-Rad, orcid.org/0000-0001-7799-9404; Abbas Yadegar, orcid.org/0000-0002-2135-7581

This article was submitted to Infectious Agents and Disease, a section of the journal Frontiers in Microbiology

Article

DOI: 10.3389/fmicb.2023.1147945

PMC ID: 9998922

PubMed ID: 36910213

SO-VID: 25530d09-a118-4dce-8713-6a22fe2907a2

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

History

Date received : 19 January 2023

Date accepted : 08 February 2023

Page count

Figures: 6, Tables: 2, Equations: 1, References: 47, Pages: 11, Words: 6311

Funding

Funded by: Shahid Beheshti University of Medical Sciences, doi 10.13039/501100005851;

This work was supported by the Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran (no. RIGLD 1061).

Intestinal microbiota changes pre- and post-fecal microbiota transplantation for treatment of recurrent Clostridioides difficile infection among Iranian patients with concurrent inflammatory bowel disease

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Abstract

Introduction

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Results and discussion

Related collections

Tick microbiome

Most cited references 47

Revised Estimates for the Number of Human and Bacteria Cells in the Body

An Ordination of the Upland Forest Communities of Southern Wisconsin

The human microbiome project.

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