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Abstract

In this study, we isolated and analyzed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The protein is synthesized in a tissue-specific manner and localized in the body muscle layer. The levels of Oo-hsp18 mRNAs are sharply induced by heat shock but not by other stressors such as levamisole and H2O2. A vaccination trial with recombinant Oo-HSP18 failed to protect calves against a challenge infection.

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PubMed ID:: 17304801

DOI:: 10.1645/GE-871R.1

ScienceOpen disciplines: Chemistry

Keywords: Amino Acid Sequence,Animals,Blotting, Western,Cattle,Cattle Diseases,prevention & control,Cells, Cultured,Cloning, Molecular,DNA, Complementary,chemistry,isolation & purification,DNA, Helminth,Gene Expression,Heat-Shock Proteins, Small,biosynthesis,genetics,immunology,Hot Temperature,Male,Molecular Sequence Data,Ostertagia,Ostertagiasis,veterinary,Polymerase Chain Reaction,Recombinant Proteins,Sequence Alignment,Sequence Homology, Amino Acid,Spodoptera,Transcription, Genetic

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