51
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Effective targeting of breast cancer cells (MCF7) via novel biogenic synthesis of gold nanoparticles using cancer-derived metabolites

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Biogenic synthesis of nanoparticles provides many advantages over synthetic nanoparticles including clean and non-toxic approaches. Nanoparticle-based application for the development of diagnostics and therapeutics is a promising field that requires further enrichment and investigation. The use of biological systems for the generation of gold nanoparticles (AuNPs) has been extensively studied. The search for a biocompatibility approach for the development of nanoparticles is of great interest since it can provide more targeting and less toxicity. Here, we reported a bio-reductive approach of gold to AuNPs using metabolites extracted from mammalian cells, which provided a simple and efficient way for the synthesis of nanomaterials. AuNPs were more efficiently synthesized by the metabolites extracted from breast cancer (MCF7) and normal fibroblasts (F180) cells when compared to metabolites extracted from cell-free supernatants. The metabolites involved in biogenic synthesis are mainly alcohols and acids. Spectroscopic characterization using UV-visible spectra, morphological characterization using electron microscopy and structural characterization using X-ray diffraction (XRD) confirmed the AuNPs synthesis from mammalian cells metabolites. AuNPs generated from MCF7 cells metabolites showed significant anticancer activities against MCF7 and low toxicity when compared to those generated from F180 cells metabolites. The results reflected the cytotoxic activities of the parent metabolites extracted from MCF7 versus those extracted from F180. Comparative metabolomics analysis indicated that MCF7-generated AuNPs harbored tetratetracontane, octacosane, and cyclotetradecane while those generated from F180 harbored a high percentage of stearic, palmitic, heptadecanoic acid. We related the variation in cytotoxic activities between cell types to the differences in AuNPs-harboring metabolites. The process used in this study to develop the nanoparticles is novel and should have useful future anticancer applications mainly because of proper specific targeting to cancer cells.

          Related collections

          Most cited references39

          • Record: found
          • Abstract: found
          • Article: not found

          Gold nanoparticles: preparation, properties, and applications in bionanotechnology.

          Gold nanoparticles (AuNPs) are important components for biomedical applications. AuNPs have been widely employed for diagnostics, and have seen increasing use in the area of therapeutics. In this mini-review, we present fabrication strategies for AuNPs and highlight a selection of recent applications of these materials in bionanotechnology. This journal is © The Royal Society of Chemistry 2012
            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Active Targeting Strategies Using Biological Ligands for Nanoparticle Drug Delivery Systems

            Targeting nanoparticle (NP) carriers to sites of disease is critical for their successful use as drug delivery systems. Along with optimization of physicochemical properties, researchers have focused on surface modification of NPs with biological ligands. Such ligands can bind specific receptors on the surface of target cells. Furthermore, biological ligands can facilitate uptake of modified NPs, which is referred to as ‘active targeting’ of NPs. In this review, we discuss recent applications of biological ligands including proteins, polysaccharides, aptamers, peptides, and small molecules for NP-mediated drug delivery. We prioritized studies that have demonstrated targeting in animals over in vitro studies. We expect that this review will assist biomedical researchers working with NPs for drug delivery and imaging.
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Biosynthesis of gold nanoparticles using the bacteria Rhodopseudomonas capsulata

                Bookmark

                Author and article information

                Contributors
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: Funding acquisitionRole: InvestigationRole: MethodologyRole: SupervisionRole: ValidationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: InvestigationRole: MethodologyRole: Writing – review & editing
                Role: InvestigationRole: Methodology
                Role: InvestigationRole: MethodologyRole: Writing – original draft
                Role: MethodologyRole: Writing – original draft
                Role: InvestigationRole: Methodology
                Role: InvestigationRole: Methodology
                Role: InvestigationRole: MethodologyRole: ResourcesRole: Writing – original draft
                Role: InvestigationRole: MethodologyRole: ResourcesRole: Writing – original draft
                Role: InvestigationRole: MethodologyRole: ResourcesRole: Writing – original draft
                Role: Editor
                Journal
                PLoS One
                PLoS One
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                6 October 2020
                2020
                : 15
                : 10
                : e0240156
                Affiliations
                [1 ] Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, UAE
                [2 ] Department of Medicinal Chemistry, College of Pharmacy, University of Sharjah, Sharjah, UAE
                [3 ] Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
                [4 ] Department of Chemistry, College of Sciences, University of Sharjah, Sharjah, UAE
                [5 ] Department of Pharmaceutics and Pharmaceutical Technology, College of Sciences, University of Sharjah, Sharjah, UAE
                [6 ] Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
                [7 ] Department of Translational Research & Cellular Therapeutics, Beckman Research Institute of City of Hope, Duarte, CA, United States of America
                [8 ] Department of Pharmacy Practice and Pharmacotherapeutics, College of Sciences, University of Sharjah, Sharjah, UAE
                [9 ] Department of Pharmacology, Faculty of Pharmacy, Beni-Suef University, Beni Suef, Egypt
                VIT University, INDIA
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                http://orcid.org/0000-0002-7691-615X
                http://orcid.org/0000-0002-7622-9683
                http://orcid.org/0000-0003-3150-9275
                Article
                PONE-D-20-19603
                10.1371/journal.pone.0240156
                7537904
                33022008
                187a0716-856d-4c7f-9aed-7fd8e4f521f8
                © 2020 Soliman et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 25 June 2020
                : 17 September 2020
                Page count
                Figures: 8, Tables: 2, Pages: 16
                Funding
                Funded by: University of Sharjah
                Award Recipient :
                This study was supported by funds from the University of Sharjah to SSMS and partially by Aljalila Foundation (grant number AJF201777) to Mohamed Haider. No additional external funding was received for this study.
                Categories
                Research Article
                Biology and Life Sciences
                Cell Biology
                Cell Physiology
                Cell Metabolism
                Biology and Life Sciences
                Biochemistry
                Metabolism
                Metabolites
                Engineering and Technology
                Nanotechnology
                Nanoparticles
                Medicine and Health Sciences
                Oncology
                Cancers and Neoplasms
                Breast Tumors
                Breast Cancer
                Research and Analysis Methods
                Chromatographic Techniques
                Gas Chromatography-Mass Spectrometry
                Physical Sciences
                Chemistry
                Analytical Chemistry
                Mass Spectrometry
                Gas Chromatography-Mass Spectrometry
                Research and Analysis Methods
                Spectrum Analysis Techniques
                Mass Spectrometry
                Gas Chromatography-Mass Spectrometry
                Physical Sciences
                Chemistry
                Chemical Compounds
                Chlorides
                Biology and Life Sciences
                Cell Biology
                Cellular Types
                Animal Cells
                Connective Tissue Cells
                Fibroblasts
                Biology and Life Sciences
                Anatomy
                Biological Tissue
                Connective Tissue
                Connective Tissue Cells
                Fibroblasts
                Medicine and Health Sciences
                Anatomy
                Biological Tissue
                Connective Tissue
                Connective Tissue Cells
                Fibroblasts
                Research and Analysis Methods
                Chemical Synthesis
                Custom metadata
                All relevant data are within the manuscript.

                Uncategorized
                Uncategorized

                Comments

                Comment on this article

                scite_
                0
                0
                0
                0
                Smart Citations
                0
                0
                0
                0
                Citing PublicationsSupportingMentioningContrasting
                View Citations

                See how this article has been cited at scite.ai

                scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

                Similar content443

                Cited by10

                Most referenced authors450